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179,553 results

Showing: 11551 - 11600 of 179553 results

  1. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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  2. pTC217

    Plasmid
    #70018
    Purpose
    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:
    Insert
    Nuclease (Cas9/sgRNA) + Donor + GVR
    Use
    CRISPR
    Expression
    Plant
    Available since
    March 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    70018-plasmid-map-sequence-id-124557

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  5. Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2

    Plasmid
    #69623
    Purpose
    A FRET sensor for Galphai1 activation, encoded on a single plasmid.
    Depositor
    Dorus Gadella
    Publication
    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Gbeta-2A-cpV-Ggamma2
    Galphai-mTurquoise2
    Tags
    ciruclar permutated Venus (cp173V) and mTurquoise…
    Expression
    Mammalian
    Promoter
    CMV and CMV (IRES)
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    69623-plasmid-map-sequence-id-459280

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  9. GPER-Tango

    Plasmid
    #66299
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPER (GPER1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    66299-plasmid-map-sequence-id-173899

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  10. ADRA2A-Tango

    Plasmid
    #66216
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ADRA2A (ADRA2A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66216-plasmid-map-sequence-id-417865

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  16. pCMV-Tag4-OMA1

    Plasmid
    #61719
    Purpose
    mammalian expression of human Oma1 with C-terminal FLAG tag
    Depositor
    Oleh Khalimonchuk
    Insert
    OMA1 (OMA1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    61719-plasmid-map-sequence-id-175330

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  18. pSR660

    Plasmid
    #65216
    Purpose
    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells
    Depositor
    Robert Kotin
    Publication
    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:
    Inserts
    modified AAV-2 rep78
    modified AAV-8 cap
    Use
    AAV
    Expression
    Insect
    Promoter
    P10 and polyhedrin
    Available since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    65216-plasmid-map-sequence-id-115626

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  19. pHLSec2-FN-YPet

    Plasmid
    #65421
    Purpose
    Expresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7
    Depositor
    Harold Erickson
    Publication
    Ohashi et al J Biol Chem. 2011 Nov 11;286(45):39188-9
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)
    Tags
    YPet
    Expression
    Mammalian
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    65421-plasmid-map-sequence-id-115435

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  20. Str-KDEL_ManII-SBP-mCherry

    Plasmid
    #65253
    Purpose
    synchronize trafficking of ManII from the ER (RUSH system)
    Depositor
    Franck Perez
    Publication
    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Streptavidin-KDEL and truncated Mannosidase II
    Tags
    Streptavidin Binding Protein (SBP) and mCherry
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    65253-plasmid-map-sequence-id-110461

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  23. pcDNA3-Lyn-AKAR4

    Plasmid
    #61620
    Purpose
    Lipid raft-targeted FRET biosensor for monitoring PKA activity
    Depositor
    Jin Zhang
    Publication
    Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-AKAR4
    Tags
    Cerulean, N-terminal targeting sequence from Lyn …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    61620-plasmid-map-sequence-id-100421

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  24. pSBtet-GH

    Plasmid
    #60498
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    60498-plasmid-map-sequence-id-97549

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  25. pSBtet-GN

    Plasmid
    #60501
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and neomycin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    60501-plasmid-map-sequence-id-97546

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  31. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60230
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Promoter
    U6 and hSyn
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    60230-plasmid-map-sequence-id-490567

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  32. pT2SC

    Plasmid
    #59382
    Purpose
    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.
    Depositor
    Shelley Copley
    Publication
    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.
    Insert
    TT-ISceI-cat
    Use
    Template
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    59382-plasmid-map-sequence-id-92124

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  34. pCAG-B19N

    Plasmid
    #59924
    Purpose
    Expression vector for Rabies virus SAD B19 nucleoprotein gene
    Depositor
    Ian Wickersham
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rabies virus nucleoprotein
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    10 Articles
    59924-plasmid-map-sequence-id-94984

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  35. mCherry-SSTR3-N-17

    Plasmid
    #55135
    Purpose
    Localization: Cilium, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SSTR3
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55135-plasmid-map-sequence-id-329523

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  36. mCherry-H2A-10

    Plasmid
    #55054
    Purpose
    Localization: Nucleus/Histones, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2A (H2AC13 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    55054-plasmid-map-sequence-id-421913

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  38. mEmerald-Rab7a-7

    Plasmid
    #54244
    Purpose
    Localization: Ras GTPase, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Rab7a (RAB7A Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    5 Articles
    54244-plasmid-map-sequence-id-298520

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  39. mEmerald-CENPA-C-22

    Plasmid
    #54035
    Purpose
    Localization: Nucleus, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CENPA (CENPA Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54035-plasmid-map-sequence-id-311824

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  41. mTagBFP-Lifeact-7

    Plasmid
    #54496
    Purpose
    Localization: Actin, Excitation: 399, Emission: 456
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lifeact-mTagBFP
    Expression
    Mammalian
    Available since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles

  45. dKeima-Red-N1

    Plasmid
    #54618
    Purpose
    Localization: N1 Cloning Vector, Excitation: 440, Emission: 616
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mKeima-Red
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only

  47. pLSLGFP.R

    Plasmid
    #51501
    Purpose
    Single component LSL vector with GFP. Rep is expressed from the complementary sense LIR promoter. GFP is between LIR and SIR and is driven from a 2X35S promoter. Rep is within the replicon
    Depositor
    Daniel Voytas
    Publication
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP
    Use
    Plant t-dna plasmid
    Promoter
    2x35S
    Available since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    51501-plasmid-map-sequence-id-261239

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Showing: 11551 - 11600 of 179553 results