295,073 results

1. TRUPATH Triple Ga13

   Plasmid

   #196062

   Purpose

   Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlpha13-RLuc8 (GNA13 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDF-Mm2

   Plasmid

   #197100

   Purpose

   To express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivati

   Depositor

   Kensaku Sakamoto

   Article

   Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

   Insert

   PylRS variant, Pyl tRNA, kanamycin resistance gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Lys61, Glu131, Ala306, Phe384, Glu444 (PylRS)

   Promoter

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDF-Mm2

   Plasmid

   #197100

   Purpose

   To express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivati

   Depositor

   Kensaku Sakamoto

   Article

   Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

   Insert

   PylRS variant, Pyl tRNA, kanamycin resistance gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Lys61, Glu131, Ala306, Phe384, Glu444 (PylRS)

   Promoter

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA5-FRT/T0-Flag-RECQL5

   Plasmid

   #222620

   Purpose

   Mammalian expression vector of flag-tagged RECQL5

   Depositor

   Daniel Durocher

   Article

   Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

   Insert

   RECQL5 (RECQL5 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. ER-GCaMP6f

   Plasmid

   #182548

   Purpose

   Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

   Depositor

   Chris Richards

   Article

   Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

   Insert

   ER localized P450
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ER-GCaMP6f

   Plasmid

   #182548

   Purpose

   Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

   Depositor

   Chris Richards

   Article

   Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

   Insert

   ER localized P450
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-hSyn-dF-HA-KORD-IRES-mCitrine (AAV8)

   Viral Prep

   #65417-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-hSyn-dF-HA-KORD-IRES-mCitrine (#65417). In addition to the viral particles, you will also receive purified pAAV-hSyn-dF-HA-KORD-IRES-mCitrine plasmid DNA. hSyn-driven, Cre-dependent KORD expression with bicistronic mCitrine, for SALB-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Article

   Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)

   Promoter

   Syn

   Tags

   mCitrine

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pEMS2022

   Plasmid

   #49129

   Purpose

   AAV plasmid with DBH promoter (PRS2/3:Con) driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-PRS2/3:Con-iCre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   DBH

   Available since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pEMS2022

   Plasmid

   #49129

   Purpose

   AAV plasmid with DBH promoter (PRS2/3:Con) driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-PRS2/3:Con-iCre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   DBH

   Available since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pLVX-opto-hCaspase-1

    Plasmid

    #208790

    Purpose

    Inducibly expresses human Caspase1, fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Caspase1 (CASP1 Human)
    

    Use

    Lentiviral

    Tags

    Cry2 mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. CAPN1

    Plasmid

    #25607

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CAPN1 (CAPN1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids 29-360

    Promoter

    Available since

    Oct. 25, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

12. CAPN1

    Plasmid

    #25607

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CAPN1 (CAPN1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids 29-360

    Promoter

    Available since

    Oct. 25, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pCDH-EF1-IRES plum

    Plasmid

    #128072

    Purpose

    gfp was exchanged to plum

    Depositor

    Oskar Laur

    Insert

    plum
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    no

    Promoter

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDH-EF1-IRES plum

    Plasmid

    #128072

    Purpose

    gfp was exchanged to plum

    Depositor

    Oskar Laur

    Insert

    plum
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    no

    Promoter

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. DLX2-hygro

    Plasmid

    #97330

    Purpose

    Generating lentiviruses to express Dlx2 and hygromycin resistance

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

    Insert

    DLX2 (Dlx2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TetO

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. DLX2-hygro

    Plasmid

    #97330

    Purpose

    Generating lentiviruses to express Dlx2 and hygromycin resistance

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

    Insert

    DLX2 (Dlx2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TetO

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TRUPATH Triple GaoB

    Plasmid

    #196052

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TRUPATH Triple GaoB

    Plasmid

    #196052

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BirA pLEMO

    Plasmid

    #119818

    Purpose

    Express endogenous biotin ligase in E.coli

    Depositor

    Alexander Gabibov

    Article

    Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

    Insert

    Biotin ligase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Rhamnose promoter

    Available since

    April 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BirA pLEMO

    Plasmid

    #119818

    Purpose

    Express endogenous biotin ligase in E.coli

    Depositor

    Alexander Gabibov

    Article

    Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

    Insert

    Biotin ligase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Rhamnose promoter

    Available since

    April 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-CAG-GFP (AAV2)

    Viral Prep

    #37825-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Promoter

    CAG

    Tags

    GFP

    Available since

    Oct. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pRL443

    Plasmid

    #70261

    Purpose

    Shuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.

    Depositor

    Peter Wolk

    Article

    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRL443

    Plasmid

    #70261

    Purpose

    Shuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.

    Depositor

    Peter Wolk

    Article

    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDFDuet-1::ADAT3-ADAT2

    Plasmid

    #15917

    Depositor

    Nina Papavasiliou

    Article

    Rubio et al Proc Natl Acad Sci U S A. 2007 May 8. 10

    Insert

    ADAT3 & ADAT2
    

    Use

    Tags

    FLAG and HexaHistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDFDuet-1::ADAT3-ADAT2

    Plasmid

    #15917

    Depositor

    Nina Papavasiliou

    Article

    Rubio et al Proc Natl Acad Sci U S A. 2007 May 8. 10

    Insert

    ADAT3 & ADAT2
    

    Use

    Tags

    FLAG and HexaHistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (AAV9)

    Viral Prep

    #105551-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (#105551). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 plasmid DNA. Expression of GFP-Cre from CamKII promoter. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    CaMKII

    Tags

    GFP

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

27. pSkylan-NS-N1

    Plasmid

    #86785

    Purpose

    for fusion of gene of interest with a monomeric reversibly photoswitchable fluorescent protein, Skylan-NS

    Depositor

    Pingyong Xu

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2016 Sep 13;11

    Type

    Empty backbone

    Use

    Tags

    Skylan-NS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSkylan-NS-N1

    Plasmid

    #86785

    Purpose

    for fusion of gene of interest with a monomeric reversibly photoswitchable fluorescent protein, Skylan-NS

    Depositor

    Pingyong Xu

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2016 Sep 13;11

    Type

    Empty backbone

    Use

    Tags

    Skylan-NS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Lenti-multi-CRISPR

    Plasmid

    #85402

    Purpose

    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Lenti-multi-CRISPR

    Plasmid

    #85402

    Purpose

    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge