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We narrowed to 7,375 results for: ef1a

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pLV-Hygro-EF1A-LivePARBackbone

Plasmid

#176526

Purpose

Expression vector with a BamHI site in-frame with a Gly-Ser linker fused to EGFP; serves as the backbone for PAR binding domain incorporation for LivePAR

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSMPUW_6.14_M4_Ef1a-HA-gateway-SV40-Zeo

Plasmid

#128450

Purpose

lentiviral destination vector with Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeo Resistance gene

Depositor

Lindy Barrett

Insert

Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeocin Resistance gene

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRRLNeo-pEF1a-p53ash-mCerulean

Plasmid

#69579

Purpose

Expresses p53 tagged with mCerulean

Depositor

Galit Lahav

Article

Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

Insert

p53 (TP53 Human)

Use

Lentiviral

Tags

mCerulean

Expression

Mammalian

Mutation

7 silent mutations in p53 DNA to prevent targetin…

Promoter

EF1alpha

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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Ef1a-DIO-BiPOLES-mCerulean

Plasmid

#154949

Purpose

Optogenetic tool for blue-light inhibition and red-light excitation of neurons

Depositor

Simon Wiegert

Article

Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

Insert

BiPOLES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Ef1-alpha

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DO-mCherry-WPRE-pA

Plasmid

#37119

Purpose

Expresses mCherry in cells lacking Cre (Cre-Off)

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

mCherry

Use

AAV and Cre/Lox; Cre-off

Tags

Expression

Mutation

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1alpha-soCoChR-tdTomato

Plasmid

#107714

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-tdTomato

Use

AAV

Tags

KA2 and tdTomato

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pMR506-EF1a-H2B-EGFP

Plasmid

#186627

Purpose

Expresses nuclear-localised EGFP under EF1a promoter. For insertion of genetic barcodes into 3'UTR of EGFP.

Depositor

Jonas Frisén

Article

Ratz et al Nat Neurosci. 2022 Mar;25(3):285-294. do

Insert

EF1a (EEF1A1 Synthetic)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-Ef1a-TVA-2A-oG

Plasmid

#172359

Purpose

Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling specifically from cre-expressing neurons

Depositor

Yoav Ben-Simon, Peter Jonas

Article

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

TVA + B19-oG

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pHage2-EF1a-TagBFP-hCoilin

Plasmid

#182589

Purpose

expresses bfp-hcoilin in mammalian cells

Depositor

Agnel Sfeir

Article

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

Coilin (COIL Human)

Use

Lentiviral

Tags

BFP

Expression

Mammalian

Mutation

Promoter

EF-1a

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN4-ires-blast

Plasmid

#167829

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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miniTol2 x EF1a_EKAREN5 + PGK-puro

Plasmid

#167817

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN5

Use

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Flp-DOG-NW

Plasmid

#75469

Purpose

Flp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Has Service

AAV1

Insert

Flp-DOG

Use

AAV and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-jGCaMP8s-WPRE

Plasmid

#176758

Purpose

AAV-mediated expression of GCaMP8s under the EF1a promoter

Depositor

Loren Looger

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

EF1a

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAID1.2-EF1a-NGFP-mAID

Plasmid

#140607

Purpose

Expression of OsTIR1 and GFP-mAID protein in mammalian cells under the control of EF1a promoter

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

OsTIR1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-SomaGCaMP7

Plasmid

#182941

Purpose

Cre-dependent expression of cell body-targeted GCaMP7f, under EF1a promoter. As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

Soma-GCaMP7

Use

AAV

Tags

Expression

Mutation

Promoter

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAF0466 Ef1a-Nm60_attB acceptor

Plasmid

#193476

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Nm60_attB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-CD19.8H.28TM.BB.3z

Plasmid

#200675

Purpose

Modular CD8hinge-CD28transmembrane-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD8HD-CD28TM-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Mutation

Promoter

EF1a-short

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas5

Plasmid

#178878

Purpose

Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas5c-bGH polyA

Use

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas8

Plasmid

#178880

Purpose

Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas8c-bGH PolyA

Use

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas11

Plasmid

#178882

Purpose

Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas11c-bGH polyA

Use

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas7

Plasmid

#178879

Purpose

Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas7c-bGH PolyA

Use

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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p156RRL-EF1a-GFP-U3H1SatA

Plasmid

#41795

Depositor

Inder Verma

Article

Zhu et al Nature. 2011 Sep 7;477(7363):179-84. doi

Insert

satellite repeat sequence

Use

Tags

Expression

Mutation

Promoter

Available Since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

Plasmid

#98974

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 3 and Cas9(D10A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-Ef1a-Zeo

Plasmid

#227268

Purpose

Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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LLP109 pEF1a-gRNA-GFP

Plasmid

#100935

Purpose

GFP, puromycin and gRNA scaffold driven by EF1a promoter

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

gRNA scaffold

Use

Grna scaffold

Tags

Expression

Mammalian

Mutation

Promoter

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-DD-Blast

Plasmid

#175271

Purpose

MegaGate destination with C-terminal DD tag and blasticidin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Tags

Destabilization Domain

Expression

Mammalian

Mutation

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC414 - pAAV EF1a hPREP

Plasmid

#59967

Purpose

An AAV packaging vector that expresses hPREP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

Prolyl oligopeptidase (PREP Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pAID1.2-EF1a-NmCherry-mAID

Plasmid

#140603

Purpose

Expression of OsTIR1 and mCherry-mAID protein in mammalian cells under the control of EF1a promoter

Depositor

Tatsuo Fukagawa

Article

Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

Insert

OsTIR1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRRLNeo-pEF1a-p53ashL344A-mCerulean

Plasmid

#69580

Purpose

Expresses p53-L344A mutant tagged with mCerulean

Depositor

Galit Lahav

Article

Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

Insert

p53-L344A (TP53 Human)

Use

Lentiviral

Tags

mCerulean

Expression

Mammalian

Mutation

p53 L344A mutation that prevents tetramerization …

Promoter

EF1alpha

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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PB_EF1a-CasRx-msfGFP-2A-Blast

Plasmid

#226010

Purpose

CasRx protein for RNA targeting in piggyBac backbone

Depositor

Silvana Konermann

Article

Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

Insert

CasRx RNA-targeting nuclease

Use

CRISPR

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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