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Lenti-entry-puro

Plasmid

#85745

Purpose

Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

Depositor

Qin Yan

Article

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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M-NMn-VP64

Plasmid

#48676

Purpose

Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9-VP64, nuclease-null

Use

CRISPR

Tags

NLS and VP64

Mutation

nuclease-null

Promoter

CMV

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pAC93-pmax-dCas9VP160

Plasmid

#48225

Purpose

dCas9VP160 on pmax expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain

Use

CRISPR

Tags

HA Tag and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NLS-GFP

Plasmid

#104061

Purpose

AAV expression of nuclear localized GFP with SV40 NLS

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV PHP.eB

Insert

GFP

Use

AAV

Promoter

CAG

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pC1-HyPer-C199S

Plasmid

#42213

Purpose

Peroxide-insensitive version of HyPer to be used as a pH-control. Can be applied as a pH sensor.

Depositor

Vsevolod Belousov

Article

Belousov et al Nat Methods. 2006 Apr;3(4):281-6.

Insert

HYPER C199S

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-SHP2WT

Plasmid

#12283

Depositor

Anton Bennett

Article

Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

Insert

SHP-2WT (PTPN11 Human)

Tags

Myc (not canonical: KLISEEDL)

Expression

Mammalian

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-SpRY-ABE8e

Plasmid

#185671

Purpose

Expresses SpRY-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-nSpRY

Use

CRISPR

Expression

Mammalian

Mutation

SpRY D10A

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDB1 (CK2alpha)

Plasmid

#27083

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha (CSNK2A1 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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FR_HNF1A

Plasmid

#31104

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

HNF1A (HNF1A Human)

Use

Flp/frt

Tags

myc

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP-NMHC II-C

Plasmid

#10843

Depositor

Robert Adelstein

Article

Golomb et al J Biol Chem. 2004 Jan 23. 279(4):2800-8.

Insert

NMHC II-C (Myh14 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pCW57.1-FOXM1c

Plasmid

#68810

Purpose

Doxycycline inducible lentiviral vector for FOXM1c expression in mammalian cells

Depositor

Adam Karpf

Article

Barger et al Oncotarget. 2015 Jul 16.

Insert

FOXM1c (FOXM1 Human)

Use

Lentiviral; Doxycycline inducible

Tags

FLAG

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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ClopHensorN

Plasmid

#50758

Purpose

Mammalian expression vector expressing the ClopHensorN chloride and proton sensor

Depositor

Colin Akerman

Article

Raimondo et al Front Cell Neurosci. 2013 Nov 13;7:202.

Insert

ClopHensorN

Tags

Strep-II

Expression

Mammalian

Mutation

E2GFP is EGFP-T203Y

Promoter

CAG

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pHO4d-Cas9

Plasmid

#67881

Purpose

Cas9nlsHis6 Bacterial Expression construct

Depositor

Michael Nonet

Article

Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

Insert

Cas9

Use

CRISPR

Tags

His6 and T7 epitope

Expression

Bacterial

Promoter

T7

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCRISPR::rpsL

Plasmid

#44505

Purpose

A crRNA expression plasmid specific to the rpsL allele.

Depositor

Luciano Marraffini

Article

Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

CRISPR::rpsL (rpsL )

Use

CRISPR; E.coli

Available Since

April 16, 2013

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-N1

Plasmid

#60561

Purpose

Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-C1

Plasmid

#54842

Purpose

Localization: C1 Cloning Vector, Excitation: 434, Emission: 474

Depositor

Michael Davidson, Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Type

Empty backbone

Tags

mTurquoise2

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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hSERT pcDNA3

Plasmid

#15483

Depositor

Randy Blakely

Article

Ramamoorthy et al Proc Natl Acad Sci U S A. 1993 Mar 15. 9

Insert

hSERT (SLC6A4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

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pMK1334

Plasmid

#127965

Purpose

sgRNA expression vector compatible with CROP-Seq and imaging screens

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTP396

Plasmid

#149336

Purpose

Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

Depositor

Rebecca Voorhees

Article

Pleiner et al Science. 2020 May 21. pii: science.abb50

Insert

anti-GFP nanobody

Tags

Biotin acceptor peptide (Avi-tag), His14, and SUM…

Expression

Bacterial

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pTDH3-dCas9

Plasmid

#46920

Purpose

Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9

Use

CRISPR

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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