We narrowed to 16,228 results for: grn

1. pKDsgRNA-p15

   Plasmid

   #62656

   Purpose

   Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   exo, beta, gam, sgRNA
   

   Expression

   Bacterial

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO5.sgRNA.EFS.GFP

   Plasmid

   #57822

   Purpose

   3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   eGFP
   

   Use

   CRISPR and Lentiviral

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-sgRNA(MS2)

   Plasmid

   #102560

   Purpose

   Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

   Depositor

   Ying Liu

   Article

   Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-sgRNA

   Plasmid

   #71409

   Purpose

   There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI

   Depositor

   David Sabatini, Eric Lander

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. T-STOP sgRNA-1

   Plasmid

   #83346

   Purpose

   The sgRNA at the stop codon of human Brachyury(T) locus

   Depositor

   James Thomson

   Article

   Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

   Insert

   T-STOP sgRNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. TP53-KO_gRNA_1

   Plasmid

   #195130

   Purpose

   TP53-targeting gRNA in the LRG2.1 backbone

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   TP53 KO gRNA 1 (TP53 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. WT1 KI gRNA1

   Plasmid

   #92312

   Purpose

   CRISPR-GFP-gRNA for cutting WT1

   Depositor

   Sean Palecek

   Article

   Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

   Insert

   WT1 (WT1 Human)
   

   Tags

   CRISPR GFP

   Expression

   Mammalian

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. L1_lacZgRNA-Ck3

   Plasmid

   #136136

   Purpose

   L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpU6:lacZgRNA
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. LENTICRISPR-UPP1_sgRNA1

   Plasmid

   #201634

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   UPP1 (UPP1 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. p246 eSpCas9_2gRNAs_mH11

    Plasmid

    #164854

    Purpose

    gRNA vector for targeting mouse H11 locus

    Depositor

    Philip Jordan

    Article

    Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

    Insert

    gRNAs for targeting mouse H11 locus
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-GG-gRNA

    Plasmid

    #226860

    Purpose

    Human gRNA expression vector backbone with BsmBI Golden Gate site

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    Sp-gRNA
    

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pgRNAtet-lvaA

    Plasmid

    #106397

    Purpose

    Guide RNA plasmid targeting lvaA on BBR1-UP origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    sgRNA towards lvaA in Pseudomonas putida
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. L1_lacZgRNA-Ck2

    Plasmid

    #136137

    Purpose

    L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpU6:lacZgRNA
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX-sgRNA

    Plasmid

    #50662

    Purpose

    Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

    Depositor

    David Sabatini, Eric Lander

    Article

    Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

    Insert

    sgAAVS1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUltra-U6-gRNAs1-5

    Plasmid

    #178569

    Purpose

    Constitutive expression of a gRNA-tRNA array for macsGESTALT editing

    Depositor

    Christopher Lengner

    Article

    Simeonov et al Cancer Cell. 2021 Aug 9;39(8):1150-1162.

    Insert

    5 gRNA-tRNA array with U6 promoter
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. ATG16L1 sgRNA

    Plasmid

    #207563

    Purpose

    pX330 expressing Cas9 and a sgRNA targeting the ATG16L1 locus

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    GCAGCAAGTGACATGTCGTC
    

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDestTol2pA2-U6:gRNA

    Plasmid

    #63157

    Purpose

    Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Type

    Empty backbone

    Use

    CRISPR; Tol2 destination vector for gateway

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO5.sgRNA.EFS.tRFP657

    Plasmid

    #57824

    Purpose

    Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    tagRFP657
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. U6-sgRNA-GFP

    Plasmid

    #200068

    Purpose

    For the knock down of GFP with an astrocyte specific mCherry expression

    Depositor

    Baljit Khakh

    Article

    Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

    Insert

    U6-sgRNA-GFP
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    GfaABC1D-mCherry

    Promoter

    U6, GfaABC1D

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKDsgRNA-pgaC

    Plasmid

    #154919

    Purpose

    aTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes

    Depositor

    Michael Lynch

    Article

    Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

    Insert

    gRNA targeting pgaC
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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