We narrowed to 44,186 results for: gats

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pET:ACAT:GFP

Plasmid

#60740

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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Split-RESCUE-C

Plasmid

#170154

Purpose

AAV vector carrying the RESCUE-DDC-λN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

RESCUE-DDC-λN-NES

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ292-D570A

Plasmid

#129679

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b

Use

CRISPR; Gateway compatible aacas12b entry clone

Expression

Plant

Mutation

D570A; AaCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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SEPT11 sh1 shRNA

Plasmid

#122020

Purpose

Knock Down of Septin 11. It targetes Septin 11 mRNA. It knocks down all known splice variants of Sept 11 mRNAs.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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E2_pENTR_R4R3_T CTP

Plasmid

#171736

Purpose

ptpTALECD vector assembly, Entry clone 2 with attR4 and R3

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

A terminator of Arabidopsis heat shock protein, RPS5A promoter, and RecA1 plastid targeting signal

Use

Multisite gateway entry vector

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pX330_ATP1A1_G7

Plasmid

#173204

Purpose

Expresses the ATP1A1 G7 sgRNA in combination with SpCas9 to target ATP1A1 intron 17.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G7 sgRNA + SpCas9

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBW412hrpL-cIgfp

Plasmid

#61438

Purpose

pSB4A3 harbouring hrpL-rbs32-cI-lva-ter-Plam-rbs30-gfp-ter

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

HrpL-rbs32-CI-LVA-t-plam-30gfp-t

Tags

LVA

Expression

Bacterial

Promoter

HrpL

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ1360 - Intron GG3 - smu-1

Plasmid

#159806

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG3 - smu-1

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1358 - Intron GG1 - smu-1

Plasmid

#159804

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - smu-1

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pME-nCas9n-P2A-mKate2-pA

Plasmid

#109547

Purpose

Multisite gateway vector for expression of nCas9n with bicistronic expression of mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

nCas9n and mKate2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBEG R3-L4 #BGV194

Plasmid

#48954

Purpose

For inserting selection gene between AttR3 and AttL4 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Gateway cloning vector

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
C234-E08: attB2r-3xFLAG-attB3

Plasmid

#162912

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal triple-FLAG epitope tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

triple FLAG epitope tag

Use

Synthetic Biology

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E44: attB5r-SNAP-nostop-attB1r

Plasmid

#162962

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal SNAP-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

SNAP tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_AVR-Pia

Plasmid

#123653

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

AVR-Pia

Use

Golden gate

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_TH16_AVR-Pib_100

Plasmid

#121378

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

TH16_AVR-Pib_100

Use

Golden gate

Mutation

Magnaporthe oryzae

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_TH12-rn_00121641

Plasmid

#121371

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

TH12-rn_00121641

Use

Golden gate

Mutation

Magnaporthe oryzae

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_PH14-rn_00186401

Plasmid

#121369

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

PH14-rn_00186401

Use

Golden gate

Mutation

Magnaporthe oryzae

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSuper.Retro.Puro-PKN1 shRNA 4

Plasmid

#59978

Purpose

Knockdown of PKN1

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

PKN1 shRNA

Use

Retroviral

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
BB2_EF

Plasmid

#98538

Purpose

empty BB2 for transcription unit cloning

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-AVPR2-eLOV-TEVcs-FLAG-GAL4-V5

Plasmid

#104844

Purpose

b2AR-SPARK TF component

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

AVPR2-eLOV-TEVcs-FLAG-GAL4-V5

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

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C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r

Plasmid

#162967

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Dual Strep2 purification tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-PtH

Plasmid

#133391

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pC0.062

Plasmid

#119651

Purpose

Level 0 part. Terminator for luxICDABEG operon from Vibrio fischeri.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Bba_B0011

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWPI-NP1

Plasmid

#65060

Purpose

Constitutive expression of NP1

Depositor

Ramon Trullas

Article

Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

Insert

Neuronal Pentraxin 1 (Nptx1 Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

* see comments

Promoter

EF1alpha

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
p3E-Bin1b

Plasmid

#109561

Purpose

Multisite gateway vector for 5' tagging with Bin1b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Bin1b (bin1b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pC0.017

Plasmid

#119621

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

dCas9

Use

Synthetic Biology

Mutation

bp 331 C to A , 1237 A to C, 1284 C to T, 2121 C …

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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721-2µ-staHIS3

Plasmid

#40611

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

HIS3 (HIS3 Budding Yeast)

Tags

HA

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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p5E-pax3a-5.4k

Plasmid

#164966

Purpose

zebrafish pax3a -5.4k promoter

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

pax3a (pax3a Zebrafish)

Use

Gateway 5 end entry vector

Mutation

promoter -5.4k

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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rGSKmyc-pXT7

Plasmid

#15492

Depositor

Sergei Sokol

Article

Dominguez et al Proc Natl Acad Sci U S A. 1995 Aug 29. 9

Insert

GSK-3 beta (Gsk3b Rat)

Use

Xenopus rna expression

Tags

myc

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pXylA-agrCA-IV

Plasmid

#53438

Purpose

xylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pCA0.001

Plasmid

#119557

Purpose

Level 0 acceptor vector for promoters truncated to transcriptional start site with lacZ compatible with blue/white screening

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
plentiCRISPR-sgMDH1

Plasmid

#72875

Purpose

The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

sgMDH1

Use

Lentiviral

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
pXW101Plux2-32Ptet2-gfp

Plasmid

#160821

Purpose

Two-layered AHL-responsive circuit containing a TetR-based inverter

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

PJ23101-30luxR-t-Plux2-32tetR-Ptet2-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

PJ23101, Plux2, Ptet2

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-BtH

Plasmid

#133392

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-RhoAa

Plasmid

#109577

Purpose

Multisite gateway vector for 3' tagging with RhoAa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAa (rhoaa Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac K-Flag M33

Plasmid

#1992

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

M33 (Cbx2 Mouse)

Tags

Flag

Expression

Insect

Mutation

Kozak sequence added before ATG

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pXW109PmerT-Pecf11-gfp

Plasmid

#160822

Purpose

Two-layered mercury responsive circuit carrying an ECF11-based amplifier

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

PJ23109-32merR-t-PmerT-33ecf11-Pecf11-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

PJ23109, PmerT, Pecf11

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW4.21

Plasmid

#62875

Purpose

Bacterial expression of MBP-MEG-1

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MEG-1

Tags

MBP

Expression

Bacterial

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET:CCCT:GFP

Plasmid

#60738

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASp YFP Rab10 DN

Plasmid

#37709

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab10 (Rab10 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T23N (dominant negative)

Promoter

GAL4

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pRU205

Plasmid

#167685

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCpf1

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU321

Plasmid

#167681

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::asCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU320

Plasmid

#167682

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C239-E03: attB2r-IRES-ffLuc2-pA-attB3

Plasmid

#162919

Purpose

Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2 reporter using an internal ribosome entry sequence (IRES)

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

firefly luciferase with upstream IRES sequence

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-Neo-HA-MmUbc6 mut/2775

Plasmid

#11483

Depositor

Allan Weissman

Article

Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

Insert

MmUbc6 (Ube2j2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

cys 94 mutated to ser

Available Since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only
pICH47742:PtFCP:Cas9YFP

Plasmid

#104894

Purpose

Cas9, with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation

Depositor

Thomas Mock

Article

Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

Insert

Cas9

Use

CRISPR; ; golden gate assembly

Tags

YFP

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL1-PorA-4xDQNAT

Plasmid

#128392

Purpose

In vitro expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

PorA

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL1-TTc-4xDQNAT

Plasmid

#128393

Purpose

In vitro expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin fragment C domain

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pXW101Plux2-33Ptet2-gfp

Plasmid

#160820

Purpose

Two-layered AHL-responsive circuit containing a TetR-based inverter

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

PJ23101-30luxR-t-Plux2-33tetR-Ptet2-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

PJ23101, Plux2, Ptet2

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-rab3aa

Plasmid

#109703

Purpose

Multisite gateway vector for 3' tagging with rab3aa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab3aa (rab3aa Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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