175,750 results
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Plasmid#109302PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tagDepositorTypeEmpty backboneTagsOmpA 1-27, 6HisExpressionBacterialPromoterT7Available SinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only
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pUPD2
Plasmid#68161PurposeUniversal domesticator plasmid for level 0 partsDepositorInsertempty vector
UseSynthetic Biology; Domestication of dna parts for…Available SinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
p15aC-1D
Plasmid#107863PurposeBacterial expression of PISDepositorInsertPIS
TagsmycExpressionBacterialPromoterproDAvailable SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTipi2.1_Anti-Biotin [IPI-M33] Mouse/Rabbit HC
Plasmid#221349PurposeMammalian expression of Anti-Biotin [IPI-M33] Mouse/Rabbit HC; to be used with Anti-Biotin [IPI-M33] light chain (Plasmid 221350) to make the antibody.DepositorInsertAnti-Biotin [IPI-M33] Mouse/Rabbit HC
UseAffinity Reagent/ AntibodyExpressionMammalianPromoterCMVAvailable SinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTipi2.1_Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa
Plasmid#221350PurposeMammalian expression of Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa; to be used with Anti-Biotin [IPI-M33] heavy chain (Plasmid 221349) to make the antibody.DepositorInsertAnti-Biotin [IPI-M33] Mouse/Rabbit LC kappa
UseAffinity Reagent/ AntibodyExpressionMammalianPromoterCMVAvailable SinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
NXPE1_pcDNA3.4-TOPO
Plasmid#226841PurposeExpresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.DepositorInsertNXPE1 (NXPE1 Human)
Tagsx6 HIS and an IL2 secretion signal both N termina…ExpressionMammalianMutationCodon optimized for expression in human cellsAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HBV 1.3-mer X-null replicon
Plasmid#65461Purposecontaining 1.3 units of the X-null HBV genome (subtype ayw)DepositorInsert1.3 units of the X-null HBV genome
ExpressionMammalianAvailable SinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ApoE2
Plasmid#197146PurposeExpresses ApoE2 in Mammalian cellsDepositorAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
AVPR2-DuET
Plasmid#213188PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-NRG1B1
Plasmid#120287PurposeProduce human recombinant NRG1 in DE3 cellsDepositorInsertNRG1-beta 1
Tags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE7.10-P2A-GFP
Plasmid#162509PurposeExpress ABE7.10-P2A-GFP by CMV promoter in mammalian cellsDepositorInsertABE7.10-P2A-GFP
UseCRISPRTagsNLSExpressionMammalianPromoterCMVAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)
Plasmid#179115PurposeCargo EGFP with AttP Bxb1 site (Parent minicircle)DepositorInsertCargo EGFP with AttP Bxb1 site
UseCRISPRAvailable SinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-Cre-GFP
Plasmid#68544PurposeAAV expression of Cre-GFP fusionDepositorInsertCre
UseAAV and Cre/LoxTagsEGFPExpressionMammalianPromoterCMVAvailable SinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
cytoplasmic EKAR (EGFP-mRFP)
Plasmid#18680DepositorInsertERK Activity Reporter
TagsEGFP and mRFPExpressionMammalianAvailable SinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ERKKTRClover
Plasmid#59138PurposeORF encoding ERK KTR mClover flanked by Gateway sequencesDepositorInsertERK Kinase Translocation Reporter (MAPK1 Human, Mouse)
Available SinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-M-SPOTIT2
Plasmid#191489PurposeGreen fluorescent-based opioid sensor for the mu opioid receptor in AAV viral vector under a CAG promoterDepositorInsertM-SPOTIT2
UseAAVPromoterCAGAvailable SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYL-kasOp-FnCas12a2
Plasmid#177874PurposeExpresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.DepositorInsertStreptomyces codon optimized FnCas12a
UseCRISPRPromoterkasOp*Available SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
iMbody_pSANG10-3F
Plasmid#184496PurposeExpresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteriaDepositorInsertiMbody
Tags3xFLAG, 6xHis, and pelB leaderExpressionBacterialAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.iGluSnFR3.v857.GPI (AAV1)
Viral Prep#178331-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.hSyn.iGluSnFR3.v857.GPI (#178331). In addition to the viral particles, you will also receive purified pAAV.hSyn.iGluSnFR3.v857.GPI plasmid DNA. Synapsin-driven, third generation glutamate sensor iGluSnFR3, variant 857. These AAV preparations are suitable purity for injection into animals.DepositorAvailable SinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRDB_270
Plasmid#231111PurposeC>T base editorDepositorInsertTadA-CDd-nCas9-SpG-UGI-HA
UseCRISPR and LentiviralMutationD10AAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT2/SV-Venus-SIINFEKL
Plasmid#111624PurposeSleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studiesDepositorInsertVenus-LEQLE-SIINFEKL-TEW
TagsLEQLE-SIINFEKL-TEWExpressionMammalianPromoterSV40Available SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHPHS800
Plasmid#228240PurposeBxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombinationDepositorInsertCas9
TagsHygromycin phosphotransferaseExpressionMammalianPromoternoneAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFREE_Amp
Plasmid#92051PurposeAllows inducible expression of gRNAs and Cas9 for plasmid curing.DepositorInsertsCas9
gRNA array
UseCRISPRPromoterpRhamBAD and ptetAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
SIRT5 Flag
Plasmid#13816PurposeMammalian expression of human SIRT5 with flag tagDepositorAvailable SinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
psgRNA
Plasmid#114005Purposeexpress sgRNA. ColE1, KanDepositorInsertgRNA
PromoterBBa_J23119Available SinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Ga13
Plasmid#196062PurposeEncodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
CTCF-C CRISPR
Plasmid#140647PurposeCTCF tagging CRISPRDepositorAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSAM_Ec
Plasmid#102939PurposeTransposon mutagenesis and Tn-seqDepositorInsertmariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
ExpressionBacterialAvailable SinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR
Plasmid#64927PurposeFRET-based biosensor for monitoring mTORC1 kinase activity.DepositorAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-IDH1-FLAG
Plasmid#66802PurposeTet-inducible expression of IDH1 in mammalian cells, 3rd generation lentiviral vectorDepositorAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TID-HA3-KanMX6
Plasmid#227980PurposeTo insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, KanMX6 markerDepositorInsertTurboID
TagsHA tagExpressionYeastAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
HiFi Cas9
Plasmid#188490PurposeExpresses FLAG tagged HiFi Cas9DepositorInsertCas9
UseCRISPR and LentiviralExpressionMammalianMutationR691APromoterEF1aAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
A24M04 Ig kappa light chain-pFEEKW
Plasmid#234810PurposeExpress Ig kappa light chain containing the VL region of the monoclonal antibody, A24M04, specific for human papilloma virus16 L1DepositorUseLentiviralExpressionBacterial and MammalianPromoterEEK promoterAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb
Plasmid#175534PurposeExpression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cellsDepositorInsertIgKchL_HA-HaloTag7-myc_PDGFRtmb
ExpressionMammalianAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_ABEmax
Plasmid#112095PurposeA:T-to-G:C base editingDepositorInsertABEmax
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMIA22
Plasmid#214448PurposeExpresses BxbI-BP-NLSDepositorInsertBxbI
TagsBP-NLSExpressionMammalianPromoterCMVAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiCHATe27_ChR2_mCherry (AAV1)
Viral Prep#213830-AAV1PurposeReady-to-use AAV1 particles produced from pAAV_BiCHATe27_ChR2_mCherry (#213830). In addition to the viral particles, you will also receive purified pAAV_BiCHATe27_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the cholinergic neuron-targeting enhancer E27. These AAV preparations are suitable purity for injection into animals.DepositorTagsmCherryAvailable SinceSept. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG2-EGFP-C-IFT122
Plasmid#218737PurposeExpresses C-terminally EGFP-tagged IFT122 in mammalian cellsDepositorAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_eGFP_TIA1
Plasmid#106094Purposegene expression of EGFP-TIA1DepositorAvailable SinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
NL4-3 mCherry Luciferase
Plasmid#44965PurposeHIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.DepositorInsertmCherry, Luciferase, IRES
UseLentiviralTagsLuciferase, IRES and mCherry with D212GMutationS34C mutation in NefAvailable SinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only