167,948 results

1. pJC175e-DddI

   Plasmid

   #179693

   Purpose

   Mutagenesis plasmid with constitutive bacterial expression of DddI immunity protein

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   [SD8] gIII
   

   Expression

   Bacterial

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MLNR-DuET

   Plasmid

   #213339

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   MLNR (MLNR Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. HB101 endA::frt lambda pir

   Bacterial Strain

   #45472

   Depositor

   Michele Swanson

   Article

   Bryan et al Mol Microbiol. 2011 Apr;80(1):231-47. do

   Bacterial Resistance

   Streptomycin

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pLenti-PalmGRET

   Plasmid

   #158221

   Purpose

   BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.

   Depositor

   Charles P. Lai

   Article

   Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

   Insert

   PalmGFP-Nluc
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. TUBB5-Halo

   Plasmid

   #64691

   Purpose

   Expresses beta-tubulin labeled with Halo-tag

   Depositor

   Yasushi Okada

   Article

   Uno et al Nat Chem. 2014 Aug;6(8):681-9. doi: 10.1

   Insert

   TUBB
   

   Tags

   Halo-tag

   Expression

   Mammalian

   Available Since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRX-IB-TMEM192-mKeima

   Plasmid

   #215359

   Purpose

   Probe to evaluate lysophagic activity

   Depositor

   Tamotsu Yoshimori

   Article

   Shima et al J Cell Biol. 2023 Dec 4;222(12):e2022040

   Insert

   transmembrane protein 192 (TMEM192 Human)
   

   Use

   Retroviral

   Tags

   mKeima

   Expression

   Mammalian

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGRG36

   Plasmid

   #16666

   Depositor

   Nancy Craig

   Article

   McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

   Insert

   mTn7::MCS
   

   Expression

   Bacterial

   Available Since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSico-shNKCC1

   Plasmid

   #83027

   Purpose

   expresses an shRNA against NKCC1

   Depositor

   Angelique Bordey

   Article

   Young et al J Neurosci. 2012 Sep 26;32(39):13630-8.

   Insert

   shNKCC1
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-CXCR4

   Plasmid

   #98942

   Purpose

   Mammalian expression plasmid for untagged human CXCR4

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CXCR4 (CXCR4 Human)
   

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496)

    Plasmid

    #214858

    Purpose

    Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Has Service

    AAV PHP.eB

    Insert

    SYFP2
    

    Use

    AAV

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Mouse NHERF-1

    Plasmid

    #32950

    Depositor

    Edward Weinman

    Article

    Weinman et al Biochim Biophys Acta. 1999 Oct 6;1447(1)

    Insert

    Slc9a3R1 (Nherf1 Mouse)
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    t3

    Available Since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. iRFP-H2A

    Plasmid

    #158691

    Purpose

    This plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markers

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    iRFP670
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA GFP10-ZIP-GFP11

    Plasmid

    #182243

    Purpose

    Tripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)

    Depositor

    Stéphanie Cabantous

    Article

    Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

    Insert

    GFP10-ZIP-11
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMP71-CD40L

    Plasmid

    #174595

    Purpose

    Stably expresses the extracellular and transmembrane domain of murine CD40L

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    CD40L
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. SadCas9 KRAB

    Plasmid

    #188504

    Purpose

    Expresses FLAG tagged SadCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV5 BRGI-Flag

    Plasmid

    #19143

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. G1A

    Plasmid

    #24690

    Depositor

    Jeanne Lawrence

    Article

    Clemson et al J Cell Biol. 1996 Feb . 132(3):259-75.

    Insert

    human XIST (XIST Human)
    

    Use

    Plasmid cloning vector

    Mutation

    Genomic insert. Includes introns 5-8.

    Available Since

    Jan. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-SaCas9

    Plasmid

    #178901

    Purpose

    SaCas9 (Cas9 from Staphylococcus aureus)

    Depositor

    Hendrik Milting

    Article

    Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

    Insert

    SaCas9
    

    Tags

    His6-Tag, bipartite nuclear localization signal f…

    Expression

    Bacterial

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a(+)/FbGH30

    Plasmid

    #86463

    Purpose

    Expression of the Beta-1,6-Exo-Laminarinase FbGH30 in E.coli

    Depositor

    Jan-Hendrik Hehemann

    Article

    Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

    Insert

    FbGH30
    

    Tags

    His-Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.3_OCT4

    Plasmid

    #26816

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

    Insert

    5'UTR-OCT4-3'UTR (POU5F1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pSG5-hPXR-K62Q

    Plasmid

    #67766

    Purpose

    Expression hPXR mutant K62Q in Mammalian Cells

    Depositor

    Sridhar Mani

    Insert

    human pregnane X receptor (PXR) (NR1I2 Human)
    

    Expression

    Mammalian

    Mutation

    lysine 62 to Glutamine

    Promoter

    SV40

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCDNA3-hPXR-FL-T248E

    Plasmid

    #41617

    Depositor

    Sridhar Mani

    Insert

    hPXR-FL-T248E (NR1I2 Human)
    

    Expression

    Mammalian

    Mutation

    N10D and T248E

    Promoter

    CMV

    Available Since

    March 11, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pET28a(+)/FbGH17A

    Plasmid

    #100911

    Purpose

    Expression of the Beta-1,3-Endo-Laminarinase FbGH17A in E.coli

    Depositor

    Jan-Hendrik Hehemann

    Insert

    FbGH17A
    

    Tags

    His-Tag

    Expression

    Bacterial

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCN34e-yfp

    Plasmid

    #227641

    Purpose

    Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    yfp
    

    Expression

    Bacterial

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCN34e-kimA-yfp

    Plasmid

    #227638

    Purpose

    c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    kimA-yfp
    

    Expression

    Bacterial

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMXs-B1

    Plasmid

    #31705

    Depositor

    Duanqing Pei

    Article

    Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

    Insert

    miR-106a
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

27. RUBYm2

    Plasmid

    #232175

    Purpose

    Introduce a C-to-T mutation to generate an early stop codon (Q139 to stop), thereby interrupting RUBY expression

    Depositor

    Yiping Qi

    Article

    Sexton et al Plant Physiol. 2025 Jul 3;198(3):kiaf265

    Insert

    RUBY mutant2 and CaMV 35S promoter
    

    Expression

    Plant

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. RUBY-ABE#2

    Plasmid

    #232176

    Purpose

    T-DNA vector with ecTadA8e-zCas9D10A and corresponding sgRNA to correct RUBYm2 and recover a vivid red color visible to the naked eyes.

    Depositor

    Yiping Qi

    Article

    Sexton et al Plant Physiol. 2025 Jul 3;198(3):kiaf265

    Insert

    2x35s-ecTadA8e-SpCas9-D10A-AtU3-sgRNA-mis4-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3xflag and NLS

    Expression

    Plant

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA 3.3 N-MYC RMDN3

    Plasmid

    #227878

    Purpose

    Used for transfection of cells for expression of C-MYC RMDN3

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

    Insert

    RMDN3 (RMDN3 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Available Since

    July 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. pMTKM30

    Plasmid

    #233502

    Purpose

    Level 0 plasmid for MoClo, encodes inducible TDH3-tetO2p for K. marxianus

    Depositor

    Vincent Martin

    Article

    Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

    Insert

    TDH3-tetO2p
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    addition of two tetracycline operator sequeneces

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMTKM31

    Plasmid

    #233503

    Purpose

    Level 0 plasmid for MoClo, encodes inducible TEF3-tetO2p for K. marxianus

    Depositor

    Vincent Martin

    Article

    Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

    Insert

    PGK1-tetO2p
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    addition of two tetracycline operator sequeneces

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAVLINKv1-pCALM1-Cre-3' ROBO2

    Plasmid

    #236687

    Purpose

    3' AAVLINK plasmid for ROBO2 expression

    Depositor

    Zhonghua Lu

    Insert

    ROBO2 (ROBO2 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG

    Promoter

    pCALM1

    Available Since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. EF1a-mGL(miRE.FF4)-bGH

    Plasmid

    #235264

    Purpose

    microRNA expression for dual-vector iFFL circuit

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mGreenLantern
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1a (human EF1a)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. xenCGN-Myc-His

    Plasmid

    #236505

    Purpose

    For expression of Xenopus CGN in Insect Cells

    Depositor

    Sandra Citi

    Article

    D'Atri et al J Biol Chem. 2002 Aug 2;277(31):27757-64

    Insert

    CGN (cgn Frog)
    

    Tags

    Myc-His

    Expression

    Insect

    Mutation

    WT

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAIP4_6IYE

    Plasmid

    #234237

    Purpose

    Heterologous protein expression of peptidyl-tRNA hydrolase (PTH) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    6IYE (pth Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pcDNA5 FRT TO GFP11-LRRK2

    Plasmid

    #231174

    Purpose

    Expresses GFP-11-LRRK2 (wildtype, full length) in mammalian cells

    Depositor

    Samara Reck-Peterson

    Article

    Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

    Insert

    GFP11-LRRK2 (LRRK2 Human)
    

    Tags

    GFP11

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pWZL-Blast FAP S624A

    Plasmid

    #207402

    Purpose

    Expresses the S624A mutant of FAP (Fibroblast Activation Protein), which shows no protease activity.

    Depositor

    Jing Yang

    Article

    Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

    Insert

    Fibroblast Activation Protein Alpha (FAP Human)
    

    Use

    Retroviral

    Mutation

    changed Serine 624 to Alanine. It's a protea…

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pWZL-Blast FAP A657S

    Plasmid

    #207403

    Purpose

    Expresses the A657S mutant of FAP (Fibroblast Activation Protein), which shows partial protease activity.

    Depositor

    Jing Yang

    Article

    Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

    Insert

    Fibroblast Activation Protein Alpha (FAP Human)
    

    Use

    Retroviral

    Mutation

    changed Alanine 657 to Serine. It's an endop…

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-EF1a-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

    Plasmid

    #220613

    Purpose

    Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG; ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

40. LHZ1494

    Plasmid

    #222104

    Purpose

    Triple sgRNAs expression plasmid in Kluyveromyces marxianus.

    Depositor

    Yongming Wang

    Insert

    sg1RNA, sg4RNA, sg7RNA
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge