166,485 results

1. GG-EBNA-OSK2M2L1-PP

   Plasmid

   #102902

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   OSK2M2L1-gRNAs-PGK-puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJM60 FLAG-RAB35 S22N

   Plasmid

   #107106

   Purpose

   Lentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

   Depositor

   Charles Sawyers

   Article

   Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

   Insert

   RAB35 (RAB35 Human)
   

   Use

   Lentiviral

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC014 - LwCas13a-msfGFP

   Plasmid

   #91902

   Purpose

   Expresses active LwCas13a for mammalian RNA targeting

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a
   

   Use

   CRISPR

   Tags

   NLS and msfGFP-NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-hLbCpf1(TYCV) (pY230)

   Plasmid

   #89355

   Purpose

   Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   G532R/K595R

   Promoter

   CMV

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EWB-DIO-myrTagRFP-T-P2A-post-eGRASP

   Plasmid

   #111581

   Purpose

   An AAV vector that expresses double floxed myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   myrTagRFP-T-P2A-post-eGRASP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available Since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-CAG-flex-GFP-4x6T

   Plasmid

   #196418

   Purpose

   Cre-dependent AAV expression of GFP preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Insert

   GFP
   

   Use

   AAV and Cre/Lox; Astrocyte-selective

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPR-EGFP

   Plasmid

   #75159

   Purpose

   Derived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistance

   Depositor

   Beat Bornhauser

   Article

   McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Go1-CASE

   Plasmid

   #168123

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG9

   Expression

   Mammalian

   Mutation

   NLuc is inserted at L91/G92 within GNAO1

   Promoter

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. makeTCR_Lentiviral_Backbone

   Plasmid

   #233943

   Purpose

   backbone for makeTCR assembly

   Depositor

   Michael Platten

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a (long)

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. STICR_Index1

    Pooled Library

    #186334

    Purpose

    High Complexity Lentiviral Barcode Library

    Depositor

    Tomasz Nowakowski

    Article

    Delgado et al Nature. 2021 Dec 15. pii: 10.1038/s41586

    Expression

    Mammalian

    Species

    Synthetic

    Use

    Lentiviral

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

11. pUK21

    Plasmid

    #49788

    Purpose

    E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

    Depositor

    Joachim Messing

    Article

    Vieira et al Gene. 1991 Apr;100:189-94.

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lacZ

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

    Plasmid

    #162378

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    A25G F286Y

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRE:GFP

    Plasmid

    #163036

    Purpose

    FLiCRE reporter gene for neuronal expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. 7TFP CDH1 reporter

    Plasmid

    #91704

    Purpose

    lentiviral luciferase reporter containing CDH1 promoter

    Depositor

    Bob Weinberg

    Article

    Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

    Insert

    CDH1 promoter (CDH1 Human)
    

    Use

    Lentiviral and Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CDH1

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-iGlucoSnFR-TS

    Plasmid

    #127952

    Purpose

    Expresses the iGlucoSnFR-TS biosensor for intracellular [glucose], with a fluorescence-lifetime readout, in mammalian cells

    Depositor

    Gary Yellen

    Article

    Diaz-Garcia et al J Neurosci Res. 2019 May 20. doi: 10.100

    Insert

    iGlucoSnFR-TS
    

    Use

    AAV

    Tags

    His6 and Xpress epitope

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG1.1

    Plasmid

    #173685

    Purpose

    The expression vector for genes of interest under CAG promoter.

    Depositor

    Masahito Ikawa

    Article

    Ikawa et al J Biol Chem. 2011 Feb 18;286(7):5639-46.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP_sfCherry2(11)_H2B

    Plasmid

    #82605

    Purpose

    Expresses sfCherry2(11) tagged H2B in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(11)_H2B
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Gly12 to Ala in sfCherry11+

    Promoter

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMF440

    Plasmid

    #62550

    Purpose

    Broad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.

    Depositor

    Michael Franklin

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1i

    Plasmid

    #233080

    Purpose

    Expression vector for sgRNA-S1b and eCas12f1i

    Depositor

    Kyoungmi Kim

    Article

    Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

    Insert

    U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1i-2xFLAG-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, CBh

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-cT3G-cERP2-EGR1

    Plasmid

    #192913

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of EGR1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    EGR1 (EGR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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