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TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro

Plasmid

#211462

Purpose

Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

TRE

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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NUMB2-eGFP-M107K

Plasmid

#37803

Depositor

Rolf Bjerkvig

Article

Euskirchen et al Exp Cell Res. 2011 Dec 10;317(20):2864-7

Insert

NUMB isoform 2 (NUMB Human)

Use

Lentiviral

Tags

eGFP

Promoter

pCMV

Available Since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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NUMB4-eGFP-M107K

Plasmid

#37805

Depositor

Rolf Bjerkvig

Article

Euskirchen et al Exp Cell Res. 2011 Dec 10;317(20):2864-7

Insert

NUMB isoform 4 (NUMB Human)

Use

Lentiviral

Tags

eGFP

Promoter

pCMV

Available Since

March 13, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR2b-mTurquoise2

Plasmid

#191014

Purpose

Gateway entry vector with the mTurquoise2 fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

Insert

mTurquoise2

Use

Gateway entry vector

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEM mouse dbx-1 [TJ#11]

Plasmid

#16269

Depositor

Thomas Jessell

Article

Pierani et al Cell. 1999 Jun 25. 97(7):903-15.

Insert

dbx1 3'UTR (Dbx1 Mouse)

Expression

Bacterial

Available Since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
pCAG-OSF-PP

Plasmid

#154247

Purpose

backbone with PreScission protease-cleavable N-terminal OneStrepFLAG tag

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Type

Empty backbone

Tags

FLAG and Strep-Tag II

Expression

Mammalian

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLK65

Plasmid

#37257

Depositor

Ann Hubbard

Article

Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

Insert

vang-like 2 (VANGL2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
Reb_206 aka PAS629

Plasmid

#71234

Purpose

Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

mNeonGreen to second copy RebB, soluble mCherry a…

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pPI356

Plasmid

#207449

Purpose

Extrachromosomal expression vector PkgE-PH-GFP and PTEN-mCherry

Depositor

Rob Kay

Article

Lutton et al Curr Biol. 2023 Aug 7;33(15):3083-3096.e

Inserts

PkgE
PTEN

Use

Unspecified

Tags

GFP and mCherry

Mutation

PH domain only

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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IDP456

Plasmid

#87002

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

barcode landing site-mCherry-4xboxB

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP8

Plasmid

#18066

Depositor

Edward Yeh

Article

Gao et al Nat Cell Biol. 2006 Oct . 8(10):1171-7.

Insert

SENP8 (SENP8 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
SAFB1-EGFP

Plasmid

#32746

Depositor

Steffi Oesterreich

Article

Townson et al Clin Cancer Res. 2000 Sep;6(9):3788-96.

Insert

SAFB1 (SAFB Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only
miR155 in pcDNA3.1

Plasmid

#78126

Purpose

Mammalian expression of miR155

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

miR155 (MIR155 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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IDP432

Plasmid

#87000

Purpose

plasmid for cell culture expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Insert

SNAP-Nlg1AB-1xλN(i)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-mTurquoise2

Plasmid

#215539

Purpose

Expression vector with the mTurquoise2 fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

mTurquoise2

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-EGFP

Plasmid

#215537

Purpose

Gateway entry vector with the EGFP fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

EGFP

Use

Gateway entry vector

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-TagGFP2

Plasmid

#121426

Purpose

TAgGFP2 was amplified from pLenti-Origene-Nrf21 and inserted into XhoI and EcoRI digested pLenti-Origene-Nrf21.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

empty TagGFP2

Use

Lentiviral

Tags

TagGFP2

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_TmiRc3

Plasmid

#25748

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pMF1707

Plasmid

#125584

Purpose

Expresses cytosolic roGFP2 (c-roGFP2) in mammalian cells

Depositor

Marc Fransen

Article

Ivashchenko et al Mol Biol Cell. 2011 May;22(9):1440-51. d

Insert

c-roGFP2

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-F0ATPMLS-mCherry-GFP1-10

Plasmid

#211466

Purpose

Lentivirus backbone expressing mCherry and GFP1-10 fusion protein with a mitochondrial localization signal

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

mito-mCherry-GFP1-10

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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bRA89

Plasmid

#100950

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PRExpress-Bxb1-hsp70pA

Plasmid

#158391

Purpose

heat shock inducible Bxb1 integrase for drosophila melanogaster

Depositor

Michael Elowitz

Article

Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

Insert

Bxb1

Expression

Insect

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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UAS-Ceru-10unit

Plasmid

#158389

Purpose

10-unit intMEMOIR recording array for Drosophila melanogaster

Depositor

Michael Elowitz

Article

Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

Insert

10unit recording array

Expression

Insect

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TTmcs

Plasmid

#25755

Purpose

Entry vector with TRE-tight promoter followed by multiple cloning site.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330_DDX4-cterm

Plasmid

#222520

Purpose

Cas9/sgRNA plasmid for targeting DDX4

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

Cas9, DDX4 sgRNA (DDX4 Synthetic, Human)

Use

CRISPR

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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dCas9-AsLOV2x3-NLSx3

Plasmid

#231566

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

dCas9-AsLOV2x3

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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AviTag-SpyCatcher

Plasmid

#72326

Purpose

Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

AviTag-SpyCatcher

Tags

N-term His6 tag and TEV cleavage site

Expression

Bacterial

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe NEO mRFP1

Plasmid

#98397

Purpose

Retroviral vector for constitutive mRFP1 expression

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

mRFP1

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Oct4

Plasmid

#19766

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

Insert

Oct4 (Pou5f1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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GpACR1-pcDNA3.1-mScarlet

Plasmid

#204964

Purpose

Expression of GpACR1 in fusion with mScarlet in mammalian cells

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

GpACR1

Tags

mScarlet

Expression

Mammalian

Promoter

CMV (+ enhancer)

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pUAS-syb:spGFP1–10

Plasmid

#73698

Purpose

Neuronal-synaptobrevin (syb):spGFP1–10 chimera for synaptic GRASP

Depositor

Marco Gallio

Article

Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

Insert

Neuronal-synaptobrevin:spGFP1–10 chimera

Use

Drosophila

Expression

Insect

Available Since

March 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD/His-miRFP703

Plasmid

#79985

Purpose

Monomeric near-infrared fluorescent protein miRFP703 in bacterial plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP703

Expression

Bacterial

Promoter

araB

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP

Plasmid

#45565

Purpose

Expresses I-Sce I nuclease in mammalian cells and BFP as a tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

I-Sce I Nuclease
BFP

Tags

HA

Expression

Mammalian

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
BlatCas9

Plasmid

#163792

Purpose

The plasmid expresses human codon-optimized BlatCas9 and gRNA expression elements.

Depositor

Yongming Wang

Article

Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_Tmcs

Plasmid

#25751

Purpose

Entry vector with TRE promoter followed by multiple cloning site.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
bRA90

Plasmid

#100951

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 marker

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMF1762

Plasmid

#125583

Purpose

Expresses mitochondrial roGFP2 (mt-roGFP2) in mammalian cells

Depositor

Marc Fransen

Article

Ivashchenko et al Mol Biol Cell. 2011 May;22(9):1440-51. d

Insert

mt-roGFP2

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM117

Plasmid

#127212

Purpose

BeYDV replicon with constitutive Luc expression, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbS9a-RFP

Plasmid

#126172

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23113

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TRE_GFP

Plasmid

#25767

Purpose

Entry vector with TRE promoter driving GFP expression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

GFP

Available Since

July 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLenti-PRSX8 eArchT3.0-EYFP

Plasmid

#89538

Purpose

Expresses eArchT 3.0-eYFP in Phox2 (including catecholamine) neurons

Depositor

Ruth Stornetta

Article

Burke et al J Physiol. 2015 Jul 1;593(13):2909-26. d

Insert

eArchT 3.0

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Promoter

PRSx8

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TTmiRc2

Plasmid

#25752

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX302 LacZ-V5 puro

Plasmid

#98579

Purpose

Lentiviral vector for constitutive expression of human LacZ with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

LacZ

Use

Lentiviral

Tags

2x V5 tag

Expression

Mammalian

Available Since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbB19c-GFP

Plasmid

#126139

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
34a_PU1 in pmiRGlo

Plasmid

#78131

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

PU1

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSW002-Pc

Plasmid

#108234

Purpose

Broad host bacterial expression vector with constitutive Pc promoter.

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

Pc promoter

Expression

Bacterial

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJCC_083 Cas12i1

Plasmid

#171670

Purpose

For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

Cas12i1

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-SENP8m

Plasmid

#18717

Depositor

Edward Yeh

Article

Gao et al Nat Cell Biol. 2006 Oct . 8(10):1171-7.

Insert

FLAG-SENP8m (SENP8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

C153A

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
pBAD/His-miRFP709

Plasmid

#79986

Purpose

Monomeric near-infrared fluorescent protein miRFP709 in bacterial plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP709

Expression

Bacterial

Promoter

araB

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pJL059

Plasmid

#177210

Purpose

Bicistronic vector with GFP reporter

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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