We narrowed to 12,110 results for: NSI
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Plasmid#160823PurposeTwo-layered AHL-responsive circuit carrying an ECF11-based amplifierDepositorInsertPJ23101-30luxR-t-Plux2-33ecf11-Pecf11-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterPJ23101, Plux2, Pecf11Available SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_024
Plasmid#123836PurposeEncodes Abscisic Acid Insensitive as a Type 4a part to be used in the MTK systemDepositorInsertABI
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_026
Plasmid#123838PurposeEncodes ha-tagged and nuclear localized Abscisic Acid Insensitive as a Type 4a part to be used in the MTK systemDepositorInsertHA::2xNLS::ABI
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_027
Plasmid#123839PurposeEncodes ha-tagged and nuclear localized Gibberellin Insensitive as a Type 4a part to be used in the MTK systemDepositorInsertHA::2xNLS::GAI
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3a_022
Plasmid#123774PurposeEncodes Gibberellin Insensitive tagged with his and tagBFP as a Type 3a part to be used in the MTK systemDepositorInserthis::GAI::tagBFP::linker
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3a_023
Plasmid#123775PurposeEncodes Absisic acid insensitive 1 taggeed with his and tagBFP as a Type 3a part to be used in the MTK systemDepositorInserthis::ABI::tagBFP::linker
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3_017
Plasmid#123732PurposeEncodes Fluorescent probe for G1/S transition as a Type 3 part to be used in the MTK systemDepositorInsertCitrine::Geminin(1-110)
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK1_019
Plasmid#123682PurposeEncodes ConL6 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector L6-2xHS4
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK1_020
Plasmid#123683PurposeEncodes ConL7 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector L7-2xHS4
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK1_012
Plasmid#123675PurposeEncodes ConL4 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector L4-2xHS4
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK1_015
Plasmid#123678PurposeEncodes ConLS, chicken hypersensitivity site 4 insulator and locus of X-over P1 (loxP) as a Type 1 part to be used in the MTK systemDepositorInsertConnector LS-2xHS4-LoxP
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI126
Plasmid#126066PurposeModule 1 vector of PlaMiNGo toolkit for expression of plastid targeted GFP1-10 in plant cellDepositorInsertChloroplastic FNR transit peptide
UseBinaryTagsGFP1-10ExpressionPlantPromoter'long' CaMV35SAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
DeltaNIRD C95S KVS-1
Plasmid#113068PurposeExpresses worm truncated KVS1.2 C113S in mammalian cellsDepositorInsertC113S K+ voltage sensitive channel subunit 1.2 (kvs-1 Nematode)
ExpressionMammalianMutationC113S+first 40 AA deletionAvailable SinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M87C448Y
Plasmid#89329PurposeInducible expression of M87 C448Y spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
ExpressionMammalianMutation1-460 bp deleted, C448YPromoterTet-responsive P tightAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP C448Y with 5'UTR
Plasmid#89325PurposeInducible expression of C448Y spastin M1 and M87 in mammalian cellsDepositorAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M1C448Y
Plasmid#89326PurposeInducible expression of M1C448Y spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
ExpressionMammalianMutation1-194 bp of 5'UTR deleted, C448YPromoterTet-responsive P tightAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-016
Plasmid#78704PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-015
Plasmid#78703PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-010
Plasmid#78697PurposeA strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.30
Plasmid#73798PurposeNabi2.30 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.30
ExpressionMammalianAvailable SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.104
Plasmid#73799PurposeNabi2.104 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.104
ExpressionMammalianAvailable SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.125
Plasmid#73800PurposeNabi2.125 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.125
ExpressionMammalianAvailable SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.216
Plasmid#73801PurposeNabi2.216 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.216
ExpressionMammalianAvailable SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.226
Plasmid#73802PurposeNabi2.226 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.226
ExpressionMammalianAvailable SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.213
Plasmid#73757PurposeNabi2.213 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.213
ExpressionMammalianAvailable SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSCKtheoRaj12
Plasmid#69942PurposepSTC2,TheoHHAzRaj12,cisRaj12-sfGFP, kanRDepositorInsertTheoHHAzRaj12,cisRaj12,sfGFP
UseSynthetic BiologyAvailable SinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCKtheoRaj11
Plasmid#69939PurposepSTC1,TheoHHAzRaj11,cisRaj11-sfGFP, kanRDepositorInsertTheoHHAzRaj11,cisRaj11,sfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCKbreak12
Plasmid#69938PurposepSTC2,cisRaj12-sfGFP, KanRDepositorInsertCisRaj12-sfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[S1Apt]
Plasmid#68425PurposeTransient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct with S1 streptavidin aptamer
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhuman U6Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJexpress414_OmpA171_WTnoGirdles
Plasmid#62653PurposeWild-type OmpA with aromatic girdle residues replaced by leucineDepositorInsertOmpA_noGirdles
UseSynthetic BiologyTags6His and FlAsHExpressionBacterialMutationAromatic girdle residues mutated to Leu (W7, W15,…PromoterT7Available SinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
ONE-GO biosensors
Plasmid Kit#1000000224PurposeThis kit consists of ten ONE vector G protein Optical (ONE-GO) biosensor plasmids for the expression of BRET-based biosensors of G proteins activated by G-protein-coupled receptors (GPCRs).DepositorAvailable SinceJan. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCW57-MCS1-P2A-MCS2 (Hygro)
Plasmid#80922PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…ExpressionMammalianPromoterTREAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57-GFP-2A-MCS
Plasmid#71783PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…ExpressionMammalianAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
DD-DamV133A-LMNB1-IRES2-mCherry
Plasmid#159599PurposeShield-1 inducible transient mammalian expression of Dam-LMNB1 for DamID, containing the V133A mutant of Dam.DepositorInsertDD-DamV133A-LMNB1-IRES2-mCherry
ExpressionMammalianMutationDam V133A mutationPromoterCMVAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_sfGPF 150TAG
Plasmid#154765Purposeplasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAGPromoterEF1Available SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXJ-Myc-IRF1
Plasmid#232641Purposetransient expresses IRF1 in mammalian cellsDepositorAvailable SinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLAG NC
Plasmid#214675PurposeNegative control plasmid for splicing reporters consisting of MCP fused FLAG epitope tagsDepositorInsertFLAG
TagsMCP and V5ExpressionMammalianPromoterEF-1aAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-MCS1-P2A-MCS2 (Blast)
Plasmid#80921PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…ExpressionMammalianPromoterTREAvailable SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAH-POLY-eRF1(E55D)
Plasmid#157925PurposeEncodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cellsDepositorInsertPOLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
ExpressionMammalianPromoterCMVAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_mScarlet_CLASP_RGS2membrane
Plasmid#133086PurposePlasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to blDepositorInsertmScarlet-CLASP
ExpressionBacterial and YeastAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only