We narrowed to 13,844 results for: cas9 genes

1. pMpGE010-gR085

   Plasmid

   #188554

   Purpose

   Binary vector for CRISPR/Cas9 targeted to MpIGPD in Marchantia polymorpha (for Agrobacterium-mediated genetic transformation)

   Depositor

   Yutaka Kodama

   Article

   Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

   Insert

   gR085
   

   Use

   CRISPR; Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TLCV2 - sgPTEN

   Plasmid

   #202759

   Purpose

   Encodes Doxycycline inducible Cas9 and sgRNA targeting PTEN

   Depositor

   Christopher Counter

   Article

   Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

   Insert

   gRNA targeting PTEN
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. bRA66

   Plasmid

   #100952

   Purpose

   GAL1 driven Cas9. gRNA can be cloned into BplI sites.

   Depositor

   James Haber

   Article

   Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRVV661

   Plasmid

   #108282

   Purpose

   Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

   Depositor

   Richard Mann

   Article

   Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

   Insert

   PhiC31 attP sites
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRVV669

   Plasmid

   #108283

   Purpose

   Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

   Depositor

   Richard Mann

   Article

   Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

   Insert

   PhiC31 attP sites
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSFDuet1-sgRNA(siteA)

   Plasmid

   #154831

   Purpose

   sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site A in CHO cells

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Insert

   sgRNA(siteA)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. bRA77

   Plasmid

   #100953

   Purpose

   GAL1 driven Cas9. gRNA can be cloned into BplI sites.

   Depositor

   James Haber

   Article

   Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEDJ333

   Plasmid

   #177272

   Purpose

   GAL1p:Cas9:CYC1t

   Depositor

   Michael Jensen

   Article

   Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSFDuet1-sgRNA(siteT9)

   Plasmid

   #154832

   Purpose

   sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site T9 in CHO cells

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Insert

   sgRNA(siteT9)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAIO3

    Plasmid

    #137844

    Purpose

    Cas9 and gRNA expression plasmid for P. falciparum; no selection in parasites.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HiFi iFE

    Plasmid

    #190141

    Purpose

    Expresses HiFiCas9-POLB (HiFi iFE)

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    HiFi Cas9-POLB
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    SpCas9 R691A mutant, Codon Optimized for mammalia…

    Promoter

    CBh

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRVV584

    Plasmid

    #108280

    Purpose

    Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

    Depositor

    Richard Mann

    Article

    Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

    Insert

    PhiC31 attP sites
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRVV613

    Plasmid

    #108281

    Purpose

    Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

    Depositor

    Richard Mann

    Article

    Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

    Insert

    PhiC31 attP sites
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TLCV2 - sgAP2S1 #2

    Plasmid

    #202757

    Purpose

    Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1

    Depositor

    Christopher Counter

    Article

    Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

    Insert

    gRNA targeting AP2S1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TLCV2 - sgAP2M1

    Plasmid

    #202758

    Purpose

    Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2M1

    Depositor

    Christopher Counter

    Article

    Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

    Insert

    gRNA targeting AP2M1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TLCV2 - sgAP2S1 #1

    Plasmid

    #202756

    Purpose

    Encodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1

    Depositor

    Christopher Counter

    Article

    Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

    Insert

    gRNA targeting AP2S1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Tol2-TLCV2

    Plasmid

    #196331

    Purpose

    the TLCV2 plasmid with Tol2 recombination sites for integration

    Depositor

    Itamar Harel

    Article

    Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

    Insert

    Cas9-2A-eGFP
    

    Use

    CRISPR; Fish expression

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Tight TRE promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGH1110

    Plasmid

    #223682

    Purpose

    U6-CloningSite-Ef1a-Cas9-NLS-Flag-T2A-EGFP-P2A-BlastR

    Depositor

    Gaelen Hess

    Article

    Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRVV700

    Plasmid

    #119017

    Purpose

    Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

    Depositor

    Richard Mann

    Article

    Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

    Insert

    TP901-1 attP sites
    

    Use

    CRISPR; Donor vector for creation of platforms fo…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRVV609

    Plasmid

    #119014

    Purpose

    Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

    Depositor

    Richard Mann

    Article

    Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

    Insert

    TP901-1 attP sites
    

    Use

    CRISPR; Donor vector for creation of platforms fo…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRVV688

    Plasmid

    #119015

    Purpose

    Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

    Depositor

    Richard Mann

    Article

    Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

    Insert

    TP901-1 attP sites
    

    Use

    CRISPR; Donor vector for creation of platforms fo…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRVV689

    Plasmid

    #119016

    Purpose

    Donor vector for creation of TP901-1 attP-flanked platforms for cassette exchange using CRISPR/Cas9

    Depositor

    Richard Mann

    Article

    Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

    Insert

    TP901-1 attP sites
    

    Use

    CRISPR; Donor vector for creation of platforms fo…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-rMERTK-HITI

    Plasmid

    #87119

    Purpose

    Gene correction donor AAV for RCS rat. AAV backbone plasmid including exon 2 of rat Mertk and rMertkgRNA for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    U6-rMertksgRNA-Mertk(intron1-2)-nEF-GFPKASH-pA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-Ai14-luc

    Plasmid

    #87118

    Purpose

    AAV backbone plasmid including luc-pA knock-in donor and Ai14gRNA for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    U6-Ai14sgRNA-Luciferase-nEF-GFPKASH-hGHpA
    

    Use

    AAV, CRISPR, and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid

    #238007

    Purpose

    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.

    Depositor

    Simon Powell, Agnel Sfeir

    Article

    Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:

    Insert

    AAVS1 (AAVS1, PPP1R12C Human)
    

    Use

    Rna donor to study rt-dsbr

    Tags

    Expression

    Mammalian

    Mutation

    3bp insertion signature (ATC) at Cas9 cut site in…

    Promoter

    CMV

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Human Lentiviral sgRNA Library - Unknown Function

    Pooled Library

    #51043

    Purpose

    Sub-pool of Sabatini/Lander sgRNA library enriched for gene targets of unknown function.

    Depositor

    David Sabatini, Eric Lander

    Article

    Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

27. Human Lentiviral sgRNA Library - Ribosomal Proteins

    Pooled Library

    #51045

    Purpose

    Sub-pool of Sabatini/Lander sgRNA library enriched for ribosomal gene targets.

    Depositor

    David Sabatini, Eric Lander

    Article

    Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pML107-PMR1

    Plasmid

    #226264

    Purpose

    Plasmid expressing Cas9 and gRNA ACATGACCGTATCTAAACTT which targets the PMR1 gene

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    PMR1 gRNA (PMR1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pML107-LUG1

    Plasmid

    #226265

    Purpose

    Plasmid expressing Cas9 and gRNA TCTTCAAGTTACTCCAAGAG which targets the LUG1 gene

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    LUG1 gRNA (YLR352W Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pML104-LEU2-2

    Plasmid

    #226263

    Purpose

    Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene

    Depositor

    Jolanda Van Leeuwen

    Article

    Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

    Insert

    LEU2 gRNA (LEU2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEPOR1CB0022

    Plasmid

    #117537

    Purpose

    Level1 Golden Gate Cassette: estended stem sgRNA cassette for SpCas9 targeting NbPDS gene in N. benthamiana

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    sgRNA (Sp Cas9) - extended stem
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. px459V2.0_Conc2

    Plasmid

    #134451

    Purpose

    Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

    Depositor

    Daniel Stange

    Article

    Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-PEmax-SpG

    Plasmid

    #211549

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

    Insert

    PEmax-SpG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLCNICK

    Plasmid

    #84653

    Purpose

    Genome editing for Lactobacillus casei Lc2W

    Depositor

    Sheng Yang

    Article

    Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

    Inserts

    Cas9 nickase
    sgRNA
    homology arms of LC2W_2179
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCW-eGFP

    Plasmid

    #162823

    Purpose

    Doxycycline-inducible overexpression of eGFP

    Depositor

    Matthew Steinhauser

    Article

    Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    V163A

    Promoter

    Tet ON

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pICSL1P1_AtU626Ter26

    Plasmid

    #191769

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMJ920

    Plasmid

    #42234

    Depositor

    Jennifer Doudna

    Article

    Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    GFP, HA epitope, and NLS (nuclear localization si…

    Expression

    Mammalian

    Mutation

    codon-optimized synthetic DNA sequence

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGL198

    Plasmid

    #180472

    Purpose

    For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

    Depositor

    Peggy Lemaux

    Article

    Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    ZmCas9

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pX459-sgAAVS1

    Plasmid

    #184403

    Purpose

    pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    AAVS1-specific sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCas12f1

    Plasmid

    #171182

    Purpose

    Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1

    Depositor

    Kohsuke Honda

    Article

    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

    Insert

    Cas12f1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cas9 promoter from Streptococcus pyogenes

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge