We narrowed to 5,240 results for: Pet2

1. pET28C-mCherry-DDX4LCD

   Plasmid

   #204408

   Purpose

   E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   DDX4(1-236) (DDX4 Human)
   

   Use

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a-TEV-TBP6.9F34MF37MF77M

   Plasmid

   #168268

   Purpose

   Expresses human TBP6.9 with the F34M/F37M/F77M triple mutant

   Depositor

   Joseph Wedekind

   Article

   Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

   Insert

   TBP6.9
   

   Use

   Tags

   6xHis tag N-terminus, TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   changed F34M, F37M and F77M

   Promoter

   T7 promotor

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-mH6-Cas12c1

   Plasmid

   #120872

   Purpose

   Expresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12c1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lac

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET21b-Tipin-His

   Plasmid

   #23122

   Depositor

   Aziz Sancar

   Article

   Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

   Insert

   Tipin (TIPIN Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pET28a-mH6-Cas12c2

   Plasmid

   #120873

   Purpose

   Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12c2
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28C-mCherry-53BP1LCD

   Plasmid

   #204407

   Purpose

   E. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   53BP1(1208-1977) (TP53BP1 Human)
   

   Use

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28sumo-hRig-I DCARDS

   Plasmid

   #210828

   Purpose

   protein expression in ecoli

   Depositor

   Anna Pyle

   Article

   Luo et al Cell. 2011 Oct 14;147(2):409-22. doi: 10

   Insert

   hRIG-I DCARDS (DDX58) with Caspase Recruitment domains deleted (RIGI Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21-HIS-Tol2

   Plasmid

   #112486

   Purpose

   functional recombinant Tol2 protein from E.coli

   Depositor

   Stephen Ekker

   Article

   Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

   Insert

   Tol2 Transposase (tpase Synthetic)
   

   Use

   Tags

   HIS/6x

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28b(+)_rPD-L1nb

   Plasmid

   #226715

   Purpose

   Heterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Recombinant PD-L1 nanobody
   

   Use

   Tags

   HA tag and Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28_mouse-pro-IL18

    Plasmid

    #183389

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    pro-IL18 (Il18 Mouse)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET23b-Casp6-His

    Plasmid

    #11823

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 6 (CASP6 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-Ec.coaD (pESC106)

    Plasmid

    #50388

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Tadhg Begley, Erick Strauss

    Article

    Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

    Insert

    PPAT
    

    Use

    Tags

    6xHis-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-mH6-Cas12g1

    Plasmid

    #120879

    Purpose

    Expresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12g1
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-Hook3 aa 1-552

    Plasmid

    #74614

    Purpose

    Expresses a truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-552 (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21a-Core Traptavidin

    Plasmid

    #26054

    Depositor

    Mark Howarth

    Article

    Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

    Insert

    Core Traptavidin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Streptavidin with 2 amino acid mutations: S52G R5…

    Promoter

    Available since

    Aug. 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pET28a-HIV p24

    Plasmid

    #212195

    Purpose

    Bacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminus

    Depositor

    Richard C. Willson

    Insert

    Chain A, Capsid protein p24, HIV type 1
    

    Use

    Tags

    Polyhistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET23b-Casp7-His

    Plasmid

    #11825

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 7 (CASP7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pET21b-Caspase-3(C163S)

    Plasmid

    #90088

    Purpose

    Expresses caspase 3 with C163S to prevent autocatalysis in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    C163S

    Promoter

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-Ec.coaE (pESC124)

    Plasmid

    #50390

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Tadhg Begley, Erick Strauss

    Article

    Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

    Insert

    DPCK
    

    Use

    Tags

    6XHIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21 MBPx-SpyCatcher

    Plasmid

    #72327

    Purpose

    Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET21 MBPx-SpyCatcher
    

    Use

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Mutation

    MBP contains A312V, I317V mutations and 172, 173,…

    Promoter

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a SnoopTag-MBP

    Plasmid

    #72323

    Purpose

    Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a SnoopTag-MBP
    

    Use

    Tags

    N-terminal His6 tag. MBP is fused at the C-termin…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET21b Apaf1 1-591

    Plasmid

    #26642

    Depositor

    Guy Salvesen

    Article

    Pop et al Mol Cell. 2006 Apr 21. 22(2):269-75.

    Insert

    Apoptotic protease activating factor 1 (APAF1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acids 1-591

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pET28a-RBP-LOV2

    Plasmid

    #185792

    Purpose

    Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker

    Depositor

    Lizhong He

    Article

    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

    Insert

    RBP-LOV2
    

    Use

    Tags

    10xHis, TEV protease cleavage site after His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET21-Streptavidin-Glutamate_Tag

    Plasmid

    #46367

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

    Insert

    Streptavidin-Glutamate_Tag
    

    Use

    Tags

    6 glutamate tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

25. BARD1 26-327 pET28a

    Plasmid

    #12646

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    BRCA1 binding partner for a Ring domain E3 Ub-ligase (BARD1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET21a-C2m2-mKate

    Plasmid

    #208765

    Purpose

    To express C2m2-mKate in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2m2-mKate (Mfge8 Mouse)
    

    Use

    Tags

    mKate

    Expression

    Bacterial

    Mutation

    Mutated 24 and 45 lysine to asparagine in C2 doma…

    Promoter

    T7 promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-NLS-M-MLV-RT

    Plasmid

    #181804

    Purpose

    pET28a-NLS-M-MLV RT

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

    Insert

    NLS-M-MLV-RT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET21-pelB-LaM-3

    Plasmid

    #172769

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-3
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b

    Plasmid

    #122850

    Purpose

    Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    m(KS_MAT)_mouseL_ACP (Fasn Mouse)
    

    Use

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a(+)::MBP-NL3F10H

    Plasmid

    #141291

    Purpose

    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    MBP-NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3), His (x10), and MBP

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28b-HRASLS3-1-132

    Plasmid

    #100725

    Purpose

    Expression of N-terminal region of human HRASLS3 in E.coli

    Depositor

    Byung Il Lee

    Article

    Han et al Protein Expr Purif. 2010 May;71(1):103-7

    Insert

    HRASLS3 (PLAAT3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET21-pelB-LaG-30

    Plasmid

    #172754

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-30
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET28a-pro-sumo1

    Plasmid

    #25101

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    pro-SUMO1 (SUMO1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Changed Serine 2 to Alanine

    Promoter

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

34. pET28a-mH6-Cas12i1

    Plasmid

    #120882

    Purpose

    Expresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12i1
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lac

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28-SpyCatcher002-MBP

    Plasmid

    #216295

    Purpose

    Expresses SpyCatcher002 linked to maltose-binding protein in bacterial cells

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SpyCatcher002-MBP
    

    Use

    Tags

    His6 tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET21a-hAIM2-His6

    Plasmid

    #51532

    Purpose

    Express His6-tagged hAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    lac operator

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET21a-hAIM2-GST

    Plasmid

    #51528

    Purpose

    Express GST-tagged hAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    lac operator

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28b-6His-AcpS

    Plasmid

    #145376

    Purpose

    Expression of E. coli AcpS

    Depositor

    Robert Tomko

    Article

    Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

    Insert

    AcpS (acpP E. coli)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pET28a-SpyCatcher-mi3

    Plasmid

    #112255

    Purpose

    Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

    Depositor

    Mark Howarth

    Article

    Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

    Insert

    SpyCatcher-mi3
    

    Use

    Tags

    C-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28a T7pCONS sfGFP

    Plasmid

    #154462

    Purpose

    pET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

    Depositor

    Daniel Daley

    Article

    Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

    Insert

    super folder GFP
    

    Use

    Tags

    His-thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge