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pDONR221-RAB10

Plasmid

#166202

Purpose

Gateway entry vector for N. furzeri RAB10

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

RAB10

Use

Gateway Cloning

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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U_(+36GFP)-BirA

Plasmid

#71753

Purpose

U+36GFP-BirA for expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_(pos36GFP)_BirA_6XHis_LPETG

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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tol2-mpx-cxcr4b whim-gfp

Plasmid

#41089

Depositor

Anna Huttenlocher

Article

Walters et al Blood. 2010 Oct 14;116(15):2803-11. Epub

Insert

CXCR4b-WHIM (cxcr4b Zebrafish)

Tags

GFP

Expression

Bacterial

Mutation

deleted last 19 amino acids of cxcr4b

Promoter

mpx

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pREVTRE-AID

Plasmid

#121067

Purpose

Retrovirus vector expresses AID-fusion protein under TRE promoter and with hygromycin resistance selectio

Depositor

Randy Poon

Article

Ng et al Cell Cycle. 2018 Dec 23. doi: 10.1080/15

Type

Empty backbone

Use

Retroviral

Tags

AID

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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CMVTO-IL12-26Sfur-B2AD-tvmvs-KKMP

Plasmid

#179646

Purpose

TVMVP-inducible IL-12 RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

IL12 A/B

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMN-327

Plasmid

#195452

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF10

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF10 BS - minTK - mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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LP376

Plasmid

#90200

Purpose

pCMV-myc with cDNA encoding HsTRAPPC10 aa residues 1000-1259

Depositor

Lotte Pedersen

Article

Schou et al Cilia. 2014 Jun 18;3:6. doi: 10.1186/204

Insert

TRAPPC10 (TRAPPC10 Human)

Tags

Myc

Expression

Mammalian

Mutation

aa residues 1000-1259

Promoter

CMV

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-UL32

Plasmid

#162658

Purpose

Expresses UL32 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CDS_mALS

Plasmid

#136081

Purpose

Confers plant resistance to chlorsulfuron

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_mALS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSCD-GSDMA-N

Plasmid

#226234

Purpose

Express GSDMA-N domain

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

Gasdermin A (Gsdma3 Mouse)

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMN-319

Plasmid

#195447

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF1

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF1 BS - minCMV - mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-Cerulean-SKL

Plasmid

#194048

Purpose

Transient expression of Cerulean-SKL in plant cell (Peroxisome)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

Cerulean

Tags

Ser-Lsy-Leu

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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GWF094

Plasmid

#133616

Purpose

NS3aH1-mCherry

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

NS3aH1-mCherry

Expression

Mammalian

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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BII-ChiNW

Plasmid

#133382

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Double UP sfGFP to mScarlet

Plasmid

#120261

Purpose

Natively produces sfGFP, in the presence of Cre will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Insert

sfGFP/mScarletP2A-mcs

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Promoter

CAG

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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Endosome-targeted optodPLD

Plasmid

#140055

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-2xFYVE for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

2xFYVE, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

H170A (catalytic dead)

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Par6 S345A

Plasmid

#11749

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

PAR6 (Pard6a Mouse)

Tags

Flag

Expression

Mammalian

Mutation

S345A

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pMN-321

Plasmid

#195449

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF10

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF10 BS - minCMV - mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-160

Plasmid

#79840

Purpose

Expresses NLS-PpsR2-mVenus under CMV promoter

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

NLS-PpsR2-mVenus

Expression

Mammalian

Mutation

RpPpsR2 cysteine 439 changed to serine

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pQCXIZ CMV/TO DEST (w321-2)

Plasmid

#17401

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Zeo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Retroviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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258/-2.5CAT

Plasmid

#25431

Depositor

Charles P. Emerson Jr.

Article

Goldhamer et al Development. 1995 Mar . 121(3):637-49.

Insert

Myo D Promoter/Enhancer (MYOD1 Human)

Use

Reporter construct

Tags

CAT

Expression

Mammalian

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pAGT5439

Plasmid

#192351

Purpose

L0 MoClo-compatible GoldenGate-module of the OCS terminator (tOCS) with stop

Depositor

Tom Schreiber

Article

Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

Insert

TTCG_Stop-tOCS_CGCT

Expression

Plant

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pTX1-RNA0

Plasmid

#126040

Purpose

In-vitro-transcription of control RNA0 in NMR tube for "Systems NMR" analysis

Depositor

Frédéric Allain

Article

Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

Insert

RNA0

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIB CMV/TO LUC (w431-1)

Plasmid

#17475

Purpose

Retroviral Luciferase Expression vector, CMV/TO promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Luciferase and Retroviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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p416GPD-PYK1

Plasmid

#34604

Depositor

Markus Ralser

Article

Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

Insert

PYK1

Expression

Yeast

Promoter

GPD

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pMT Draper-Ala11-GFP

Plasmid

#126654

Purpose

Inducible expression of Draper-Ala11-GFP mutant in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmi Tyr residues mutated to Ala

Expression

Insect

Mutation

Cytoplasmic Tyr residues to Ala

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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rNrx1beta(47-299)

Plasmid

#20173

Depositor

Axel Brunger

Article

Arac et al Neuron. 2007 Dec 20. 56(6):992-1003.

Insert

Neurexin 1 beta (Nrxn1 Rat)

Tags

6X His tag

Expression

Insect

Mutation

no splice site

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pB6N_RB38-Mt-tk_v8_AGKS

Plasmid

#191514

Purpose

Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (N-terminal DddAN, Right)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

v8 ZF-DdCBE RB38-Mt-tk AGKS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pB7N_LT51-Mt-tk_v8_AGKS

Plasmid

#191515

Purpose

Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (N-terminal DddAC, Left)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

v8 ZF-DdCBE LT51-Mt-tk AGKS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMN-294

Plasmid

#195453

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF1

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF1 BS - ybTATA - mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pZS220

Plasmid

#177642

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

GFP

Mutation

K47A

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCS-PluxIlux

Plasmid

#47641

Purpose

wt luxI promoter in pCS26

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

PluxI

Use

Synthetic Biology

Expression

Bacterial

Promoter

PluxI

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only
p005kanRFP

Plasmid

#58533

Purpose

RFP from coral Corynactis sp. under T5 promoter with lac operators; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

Red fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

T5 between two lac operators

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-AtUBQ10:GFP-T35S

Plasmid

#211836

Purpose

Plant transient expression cassette for GFP (control) under UBQ10 promoter.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

GFP control

Use

Synthetic Biology

Tags

GFP

Expression

Plant

Promoter

UBIQUITIN10

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2 xRor2-mCherry

Plasmid

#67162

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

mCherry

Expression

Mammalian

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pRS405 VAC8-GFP

Plasmid

#166836

Purpose

Expresses Vac8-GFP in yeasts.

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

VAC8 promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SyGCaMP5G

Plasmid

#162519

Purpose

Synaptically targeted GCaMP5G

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GCaMP5G

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
3TERM_bba_b0015_rev_chlo

Plasmid

#163931

Purpose

L0 part - terminator

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

from GenBank: KT364744.3, prokaryotic double terminator BBa_B0015

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Nkx6.2

Plasmid

#15542

Depositor

John Rubenstein

Article

Qiu et al Mech Dev. 1998 Mar . 72(1-2):77-88.

Insert

Nkx6.2 (Nkx6-2 Mouse)

Expression

Bacterial

Mutation

Partial cDNA

Available Since

Aug. 20, 2007

Availability

Academic Institutions and Nonprofits only
pDONR221-VPS35

Plasmid

#166220

Purpose

Gateway entry vector for N. furzeri VPS35

Depositor

Daniel Jarosz

Article

Chen et al Dev Cell. 2024 Jul 22;59(14):1892-1911.e

Insert

VPS35

Use

Gateway Cloning

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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