We narrowed to 59,537 results for: cloning

1. pSB700 lenti gRNA Puro

   Plasmid

   #167911

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-Lb-crRNA

   Plasmid

   #78957

   Purpose

   Cloning vector for expression of LbCpf1 crRNA. It contains BsmB1 site for cloning.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   June 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKHH030

   Plasmid

   #89358

   Purpose

   hU6 sgRNA cloning vector for CDKO system

   Depositor

   Michael Bassik

   Article

   Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

   Insert

   GFP, Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-dCasRx-ccdB

   Plasmid

   #221003

   Purpose

   Destination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.

   Depositor

   Benjamin Blencowe, Mikko Taipale

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pV1200

   Plasmid

   #111429

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al Sci Adv. 2015;1(3):e1500248.

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSVL(D2m)

   Plasmid

   #29336

   Depositor

   John Taylor

   Article

   Kuo et al J Virol. 1989 May . 63(5):1945-50.

   Insert

   Dimer of HDV genome with 2 nucleotide deletion in delta antigen
   

   Expression

   Mammalian

   Mutation

   Contains a dimer of the complete HDV genome excep…

   Available Since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. p663-UBC-miniTurbo-V5-CDK14_IDG-K

   Plasmid

   #158172

   Purpose

   Gateway destination clone of CDK14 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CDK14 (CDK14 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS-iNOS

   Plasmid

   #19295

   Depositor

   Charles Lowenstein

   Article

   Lowenstein et al Proc Natl Acad Sci U S A. 1992 Aug 1. 89

   Insert

   inducible nitric oxide synthase (Nos2 Mouse)
   

   Expression

   Bacterial

   Available Since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. TCF4 in pENTR

   Plasmid

   #216569

   Purpose

   gateway cloning donor vector containing TCF4

   Depositor

   Douglas Allan, Christopher Loewen

   Insert

   TCF4 (TCF4 Human)
   

   Use

   Gateway entry plasmid

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. T444T-GFP(NCO)

    Plasmid

    #183972

    Purpose

    Enhanced RNAi clone for C. elegans knockdown of non-codon-optimized GFP

    Depositor

    David Matus

    Article

    Palmisano et al CourseSource, 2022

    Insert

    GFP (non-codon-optimized)
    

    Use

    RNAi

    Expression

    Worm

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMEXNH3

    Plasmid

    #110079

    Purpose

    Analogous to pMINTNH3, but containing oriM for episomal replication in mycobacteria.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    His10 Tag - 3C cleavage site

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p667-UBC-CDK12-V5_IDG-K

    Plasmid

    #135245

    Purpose

    Gateway destination clone of CDK12 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    CDK12-V5 (CDK12 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOGG012

    Plasmid

    #113986

    Purpose

    Vector construction module. Partition genes (parABCDE) for plasmid stability in absence of antibiotic selection for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Partition genes (parABCDE)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHCD1A

    Plasmid

    #102952

    Purpose

    gene cloning of human CD1A

    Depositor

    Gennaro De Libero, Lucia Mori

    Article

    Manolova et al Eur J Immunol. 2003 Jan;33(1):29-37.

    Insert

    CD1A cDNA (CD1A Human)
    

    Use

    Cloning

    Available Since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-EGFP-C1-HsTAB182_K

    Plasmid

    #146777

    Purpose

    Mammalian Expression of HsTAB182

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

    Insert

    HsTAB182 (TNKS1BP1 Human)
    

    Expression

    Mammalian

    Mutation

    2 silent mutations compared to the sequence given…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAbaMCi-BsaI_pJMP2776

    Plasmid

    #208890

    Purpose

    Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette
    

    Expression

    Bacterial

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. p663-UBC-V5-GSK3B_IDG-K

    Plasmid

    #158166

    Purpose

    Gateway destination clone of V5-GSK3B (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    V5-GSK3B (GSK3B Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR eGFPCreERT2 L1-R5

    Plasmid

    #43961

    Depositor

    Peter Hohenstein

    Article

    Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

    Insert

    eGFP-CreERT2
    

    Use

    Multisite gateway entr clone

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL193

    Plasmid

    #180471

    Purpose

    For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

    Depositor

    Peggy Lemaux

    Article

    Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Cas9

    Expression

    Plant

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDD285

    Plasmid

    #66826

    Purpose

    mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    mKate2-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized mKate2

    Expression

    Worm

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only