163,403 results

1. pQFlag-USP7 WT puroR

   Plasmid

   #46751

   Purpose

   Retroviral vector that expresses wild type Flag-tagged human USP7

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP7 (USP7 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCASB

   Plasmid

   #190175

   Purpose

   Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

   Depositor

   Jessica Tyler

   Article

   Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

   Insert

   sgRNA cloning site
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Added 5'-agagacc-3' upstream and 5'…

   Promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. V5-LOV-Turbo-NES

   Plasmid

   #199545

   Purpose

   expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Lentiviral

   Tags

   NES and V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. HIV-1 Gag-mCherry

   Plasmid

   #85390

   Purpose

   Visualization marker for viral core to measure cellular uptake

   Depositor

   Gregory Melikyan

   Article

   Miyauchi et al Biochem J. 2011 Mar 15;434(3):559-69. do

   Insert

   HIV-1 gag
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pREDawn-AmpR-MCS

   Plasmid

   #188978

   Purpose

   For red light-induced bacterial expression of gene of interest (AmpR)

   Depositor

   Heikki Takala

   Article

   Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AID* Kit

   Plasmid Kit

   #1000000120

   Purpose

   Expanded tool kit for the auxin-inducible degron (AID) system in yeast ( S. cerevisiae). Includes tags for easy detection by antibodies/microscopy and a set of selection markers.

   Depositor

   Helle Ulrich

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

7. FPR2-Tango

   Plasmid

   #66284

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   FPR2 (FPR2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. β3-integrin-YFP

   Plasmid

   #26653

   Depositor

   Jonathan Jones

   Article

   Tsuruta et al FASEB J. 2002 Jun . 16(8):866-8.

   Insert

   β3-integrin (ITGB3 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. peGFP NOVA2 op

   Plasmid

   #224354

   Purpose

   Express GFP-tagged human NOVA2 (codon optimized)

   Depositor

   Nicolas Charlet-Berguerand

   Article

   Mattioli et al Am J Hum Genet. 2020 Apr 2;106(4):438-45

   Insert

   NOVA2 (NOVA2 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   codon optimized for expression in human cells

   Promoter

   CMV and T7

   Available since

   Sept. 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pFUW-VenusFlag-hCD44

    Plasmid

    #211824

    Purpose

    Express CD44 in mammalian cells

    Depositor

    Haian Fu

    Article

    Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

    Insert

    CD44 (CD44 Human)
    

    Use

    Lentiviral

    Tags

    N-Terminal Venus and Flag tags

    Expression

    Mammalian

    Mutation

    isoform corresponds to UniProt ID P16070-1 with a…

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTrcAgBIS (CO)

    Plasmid

    #35153

    Depositor

    Taek Soon Lee, Jay Keasling

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    Bisabolene Synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTrc

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCXLE-Sox2-T2A-Klf4-E2A-Myc

    Plasmid

    #210018

    Purpose

    episomal SKM (expressing mouse Sox2 + Klf4 + Myc) for integration-free naïve reset

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Sox2-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-Cepheid1b-ST-WPRE

    Plasmid

    #214967

    Purpose

    Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for brighter fluorescence

    Depositor

    Peng Zou

    Article

    Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

    Insert

    Cepheid1b-ST
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRIPZ-p21-HA

    Plasmid

    #215110

    Purpose

    Expresses p21-HA in mammalian cells from an inducible lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (Tet-inducible)

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA Sec13 pRK5

    Plasmid

    #46332

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Sec13 (SEC13 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. Gi1-CASE

    Plasmid

    #168120

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG2

    Expression

    Mammalian

    Mutation

    NLuc is inserted at A121/E122 within GNAI1

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Jag1-HA pIRES

    Plasmid

    #17336

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Koutelou et al J Biol Chem. 2007 Dec 12. ():.

    Insert

    Jagged1 (Jag1 Rat)
    

    Use

    Tags

    HA X3

    Expression

    Mammalian

    Mutation

    A silent C->G mutation at 3237

    Promoter

    Available since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLM-CMV-R-Cre

    Plasmid

    #27546

    Purpose

    Lentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

    Insert

    mCherry_P2A_Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV2)

    Viral Prep

    #105545-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    HI

    Tags

    EGFP

    Available since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

20. pHTNW

    Plasmid

    #136403

    Purpose

    Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLKO.1-Tet-Puro-shIAPEz_2

    Plasmid

    #185017

    Purpose

    shRNA mediated knockdown

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    ERV_IAPEz
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLKO.1-Tet-Puro-shIAPEz_1

    Plasmid

    #185016

    Purpose

    shRNA mediated knockdown

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    ERV_IAPEz
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRBC-sfGFP-150TAG

    Plasmid

    #174076

    Purpose

    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,

    Depositor

    Ryan Mehl

    Article

    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

    Insert

    Superfolder GFP
    

    Use

    Tags

    His-6

    Expression

    Bacterial

    Mutation

    N150TAG

    Promoter

    T7

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

    Plasmid

    #183951

    Purpose

    Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

    Depositor

    Eugene Yeo

    Article

    Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pscAAV-GFP

    Plasmid

    #32396

    Depositor

    John T Gray

    Article

    Gray et al Methods Mol Biol. 2011;807:25-46.

    Insert

    GFP
    

    Use

    AAV; Adeno-associated virus

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pALPS vif

    Plasmid

    #101328

    Purpose

    Encodes codon optimized NL4-3 vif

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 vif
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HA-EglN2-pcDNA3

    Plasmid

    #18961

    Depositor

    William Kaelin

    Article

    Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

    Insert

    Egg laying Nine - 2 (EGLN2 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRN3P-NLS-EGFP-Btgh-3NLS

    Plasmid

    #194469

    Purpose

    Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.

    Depositor

    Mathieu Boulard

    Article

    Formichetti et al Dev Cell 10 Jan 2024

    Insert

    BtGH84
    

    Use

    For in vitro transcription from t3 promoter after…

    Tags

    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDEST-HisMBP

    Plasmid

    #11085

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac (lactose/IPTG inducible)

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE

    Plasmid

    #223287

    Purpose

    A complete OptoMBP cassette for dissolution of FUS-CHOP condensates.

    Depositor

    Jared Toettcher

    Article

    Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

    Insert

    myc-FUS-CHOP-FusionRed-FRB-iLID, MBP-SspB-irFP-NLS
    

    Use

    Synthetic Biology; Piggybac transposase insertabl…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28b_H6-Smt3-AesC-GFP

    Plasmid

    #234486

    Purpose

    Superfolder GFP C-terminally fused to His-Smt3-tagged AesC split intein

    Depositor

    Henning Mootz

    Article

    Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

    Insert

    C-terminal Fragment of the Aes123 PolB1 Split Intein
    

    Use

    Tags

    Hexahistidine Tag, Superfolder Green Fluorescent …

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMAL-c2X_MBP-CLmN-H6

    Plasmid

    #234487

    Purpose

    Maltose binding protein N-terminally fused to His-tagged CLmN split intein

    Depositor

    Henning Mootz

    Article

    Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

    Insert

    N-terminal Fragment of the CLm Split Intein
    

    Use

    Tags

    Hexahistidine Tag and Maltose Binding Protein (MB…

    Expression

    Bacterial

    Mutation

    T69K, F75H, M118N within the N-terminal fragment …

    Promoter

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TFORF0460

    Plasmid

    #142631

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CREB5 (CREB5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ZsGreen1-cMYC/pLVX-Puromycin

    Plasmid

    #180278

    Purpose

    Plasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).

    Depositor

    Andre Catic

    Article

    Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

    Insert

    ZsGreen1-cMYC fusion protein MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. nls-mCherry

    Plasmid

    #108881

    Purpose

    Expression of nuclear localized mCherry

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    nls-mCherry
    

    Use

    Can be used for mammalian expression or in vitro …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET N6his GFP1-9

    Plasmid

    #182240

    Purpose

    Tripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.

    Depositor

    Stéphanie Cabantous

    Article

    Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

    Insert

    GFP1-9
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET14-GFP15_Encap

    Plasmid

    #86407

    Purpose

    Expresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operon

    Depositor

    David Savage

    Article

    Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

    Inserts

    sfGFP with a 15aa CLP tag
    Encapsulin
    

    Use

    Tags

    6x Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. GFP-pcDNA3-PKCgamma-cys1Acys1B

    Plasmid

    #21190

    Depositor

    Tobias Meyer

    Article

    Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

    Insert

    PKCgamma-cys1Acys1B (Prkcg Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PKCgamma-cys1Acys1B (aa35 to aa152 of rat PKCgamm…

    Promoter

    Available since

    July 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pYPQ142

    Plasmid

    #69294

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLentiCas9-GFP

    Plasmid

    #78546

    Purpose

    Fusion protein Cas9+GFP

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Fusion protein with Cas9

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge