215,785 results

1. pU6-Sp-pegRNA-HEK3_CTT_ins

   Plasmid

   #132778

   Purpose

   S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   HEK3_CTT_ins pegRNA
   

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   U6

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. ND4-DdCBE- left side TALE

   Plasmid

   #157844

   Purpose

   expresses left-side TALE–DddAtox half ND4-DdCBE in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   SOD2 MTS–3x-HA–ND4 left tale–G1397 DddA-C–UGI–SOD2 3'UTR
   

   Expression

   Mammalian

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TMPRSS2

   Plasmid

   #53887

   Purpose

   Expression of TMPRSS2

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Has Service

   Cloning Grade DNA

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. TMPRSS2

   Plasmid

   #53887

   Purpose

   Expression of TMPRSS2

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Has Service

   Cloning Grade DNA

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pPT24-mtnluc

   Plasmid

   #184339

   Purpose

   Transformation of mitochondrial DNA in yeast

   Depositor

   Martin Ott

   Article

   Rzepka et al STAR Protoc. 2022 May 25;3(2):101359. do

   Insert

   nanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs
   

   Expression

   Yeast

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-HA-Sox2-IP

   Plasmid

   #13459

   Depositor

   Shinya Yamanaka

   Article

   Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

   Insert

   Sox2 (Sox2 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-HA-Sox2-IP

   Plasmid

   #13459

   Depositor

   Shinya Yamanaka

   Article

   Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

   Insert

   Sox2 (Sox2 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-MCP-dNS-SRRM1-V5-mCherry

   Plasmid

   #235095

   Purpose

   SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein with MCP domain

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   pCAG-MCP-dNS-SRRM1-V5-mCherry (SRRM1 Human)
   

   Expression

   Mammalian

   Mutation

   The SRRM1 entry clone from DNASU was modified usi…

   Promoter

   CAG

   Available Since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-Blasticidin

   Plasmid

   #75085

   Purpose

   retrovirus-mediated gene transfer into mammalian cells

   Depositor

   David Mu

   Article

   Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-Blasticidin

    Plasmid

    #75085

    Purpose

    retrovirus-mediated gene transfer into mammalian cells

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-multi-CRISPR

    Plasmid

    #85402

    Purpose

    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDZ2FE_Pogz_1-1410

    Plasmid

    #105674

    Purpose

    Expresses Flag-tagged full-length human POGZ protein

    Depositor

    Thanos Halazonetis

    Insert

    POGZ (POGZ Human)
    

    Tags

    2X Flag tag

    Expression

    Mammalian

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVSV-G

    Plasmid

    #138479

    Purpose

    Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    VSV-G envelop protein
    

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pULTRA-CNF

    Plasmid

    #48215

    Purpose

    highly efficient suppressor plasmid

    Depositor

    Peter Schultz

    Article

    Schultz et al J Am Chem Soc. 2006 Nov 1;128(43):13984-

    Inserts

    polyspecific MJ tyrosyl synthetase
    tRNA
    

    Expression

    Bacterial

    Mutation

    Y32L, L65V, F108W, Q109M, D158G, I159A

    Promoter

    proK and tacI

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pULTRA-CNF

    Plasmid

    #48215

    Purpose

    highly efficient suppressor plasmid

    Depositor

    Peter Schultz

    Article

    Schultz et al J Am Chem Soc. 2006 Nov 1;128(43):13984-

    Inserts

    polyspecific MJ tyrosyl synthetase
    tRNA
    

    Expression

    Bacterial

    Mutation

    Y32L, L65V, F108W, Q109M, D158G, I159A

    Promoter

    proK and tacI

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ND4-DdCBE-right side TALE

    Plasmid

    #157843

    Purpose

    expresses right-side TALE–DddAtox half ND4-DdCBE in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    COX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA-N–UGI–ATP5B 3'UTR
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. ilux pGEX(-)

    Plasmid

    #107879

    Purpose

    Bacterial expression of ilux for bioluminescence imaging

    Depositor

    Stefan Hell

    Article

    Gregor et al Proc Natl Acad Sci U S A. 2018 Jan 30;11

    Insert

    ilux
    

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXJ-Myc-GATA4

    Plasmid

    #232647

    Purpose

    transient expresses GATA4 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

    Insert

    GATA4 (GATA4 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. SPYtag-GFPnanobody

    Plasmid

    #214837

    Purpose

    This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

    Insert

    SPYtag-GFPnanobody
    

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJFRC81-10XUAS-IVS-Syn21-GFP-p10

    Plasmid

    #36432

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Proc Natl Acad Sci U S A. 2012 Apr 9.

    Insert

    GFP
    

    Expression

    Insect

    Available Since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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21. pJFRC81-10XUAS-IVS-Syn21-GFP-p10

    Plasmid

    #36432

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Proc Natl Acad Sci U S A. 2012 Apr 9.

    Insert

    GFP
    

    Expression

    Insect

    Available Since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCR1446

    Plasmid

    #111129

    Purpose

    Expresses FANCF

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCF (FANCF Human)
    

    Use

    Lentiviral

    Tags

    HA

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. CCR5-Tango

    Plasmid

    #66242

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CCR5 (CCR5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

24. CCR5-Tango

    Plasmid

    #66242

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CCR5 (CCR5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

25. NmCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122091

    Purpose

    U6 driven NmCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    NmCas9 tracrRNA (NEWENTRY Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PB-CA

    Plasmid

    #20960

    Purpose

    piggyBac empty vector with Gatweway cassette and CAG constitutive promoter

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    Gateway Destination Vector
    

    Use

    piggybac

    Expression

    Mammalian

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PB-CA

    Plasmid

    #20960

    Purpose

    piggyBac empty vector with Gatweway cassette and CAG constitutive promoter

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    Gateway Destination Vector
    

    Use

    piggybac

    Expression

    Mammalian

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHLUM

    Plasmid

    #40276

    Depositor

    Markus Ralser

    Article

    Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

    Inserts

    LEU2 (LEU2 Budding Yeast)
    URA3 (URA3 Budding Yeast)
    MET15/17 (MET17 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    endogenous LEU2, endogenous MET15/17, and endogen…

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHLUM

    Plasmid

    #40276

    Depositor

    Markus Ralser

    Article

    Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

    Inserts

    LEU2 (LEU2 Budding Yeast)
    URA3 (URA3 Budding Yeast)
    MET15/17 (MET17 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    endogenous LEU2, endogenous MET15/17, and endogen…

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA5-FRT-TOgw-C-mCherry

    Plasmid

    #223952

    Purpose

    Gateway cloning of genes of interest with a C-terminal mCherry fusion; generation of stable cells with doxycycline inducible expression

    Depositor

    Roko Žaja, Karla Feijs

    Type

    Empty backbone

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET47b-T7-enAsCas12f

    Plasmid

    #204642

    Purpose

    Expression of enAsCas12f in E. coli

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    enAsCas12f
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D196K, N199K, G276R, N328G, D364R

    Promoter

    T7

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBS-SKII-3XHA-HALO-NAT

    Plasmid

    #188931

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-HALO-NAT
    

    Expression

    Yeast

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAGGS-PDGFRb-IRES2-EGFP

    Plasmid

    #204354

    Purpose

    Expresses wild-type PDGFRb gene.

    Depositor

    Hirofumi Nakatomi

    Article

    Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

    Insert

    Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
    

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

    Plasmid

    #180012

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; HE1A: BFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pmCherry-SopF

    Plasmid

    #135174

    Purpose

    Expresses an mCherry-SopF fusion in mammalian cells

    Depositor

    Leigh Knodler

    Article

    Lau et al PLoS Pathog. 2019 Jul 24;15(7):e1007959.

    Insert

    SopF (STM1239 Salmonella enterica serovar Typhimurium)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFGAMS-EGFP

    Plasmid

    #99107

    Purpose

    Expresses human PFAS-EGFP fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    An et al Science. 2008 Apr 4;320(5872):103-6. doi

    Insert

    PFAS (PFAS Human)
    

    Tags

    EGFP (enhanced green fluorescent protein)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCRISPRi

    Plasmid

    #199808

    Purpose

    vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

    Depositor

    Steve Salipante

    Article

    Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

    Inserts

    dCas9
    sgRNA with GFP cassette
    

    Use

    CRISPR

    Promoter

    P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid

    #85165

    Purpose

    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.

    Depositor

    Robin Dowell

    Article

    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

    Insert

    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    

    Tags

    AmilCP and RTX

    Expression

    Bacterial

    Promoter

    J23100 promoter

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pKOV

    Plasmid

    #25769

    Depositor

    George Church

    Article

    Link et al J Bacteriol. 1997 Oct . 179(20):6228-37.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Aug. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pKOV

    Plasmid

    #25769

    Depositor

    George Church

    Article

    Link et al J Bacteriol. 1997 Oct . 179(20):6228-37.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Aug. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge