167,318 results

1. CDK9 gRNA (BRDN0001162576)

   Plasmid

   #77170

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDK9

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CDK9 (CDK9 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3XFlag-CAPN2

   Plasmid

   #60942

   Purpose

   Expresses calpain2 in mammalian cells

   Depositor

   Yi Zhang

   Article

   Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

   Insert

   Calpain 2 (CAPN2 Human)
   

   Tags

   3xflag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. P3-Lenti-dCas9-Tet1-GFP

   Plasmid

   #190729

   Purpose

   Lentiviral construct to express spdCas9-Tet1-GFP.

   Depositor

   Maja Jagodic

   Article

   Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

   Insert

   dCas9-tet1
   

   Use

   CRISPR

   Tags

   SV40 NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   Changed Pro434 codon from CCG to CCC and Gly468 f…

   Promoter

   UbC

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Green Pegassos

   Plasmid

   #163114

   Purpose

   Expresses biosensors for pyruvate in mammalian cells

   Depositor

   Takashi Tsuboi

   Article

   Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

   Insert

   Green Pegassos
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pet26-MBP-ELIC

   Plasmid

   #39239

   Depositor

   Raimund Dutzler

   Article

   Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

   Insert

   Eriwinia chrysanthemi - pentameric ligand gated ion channel
   

   Tags

   His-10 and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_RAF1_WT

   Plasmid

   #82317

   Purpose

   Gateway Donor vector containing RAF1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   RAF1 (RAF1 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA 3.2/V5-DEST MTFR1L-V5

   Plasmid

   #69822

   Purpose

   Expresses MTFR1L-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   Mitochondrial fission regulator 1-like (MTFR1L Human)
   

   Use

   Retroviral

   Tags

   v5

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP15079 - pAAV-AiE0127m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5079)

   Plasmid

   #230403

   Purpose

   AiE0127m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Promoter

   minBG

   Available Since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-KO-PGK-Puro

   Plasmid

   #227264

   Purpose

   Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-Neo-Bam p53 R248W

    Plasmid

    #16437

    Depositor

    Bert Vogelstein

    Article

    Baker et al Science. 1990 Aug 24. 249(4971):912-5.

    Insert

    p53 R248W (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    R248W

    Available Since

    Aug. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. SP1-SB100X

    Plasmid

    #154887

    Purpose

    Sleeping Beauty transposase fused to SP1

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

    Insert

    SP1-Sleeping Beauty
    

    Expression

    Mammalian

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. gRNA_AAVS1-T2

    Plasmid

    #41818

    Purpose

    Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_AAVS1-T2
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTT5-hSDC1

    Plasmid

    #52326

    Purpose

    expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Expression

    Mammalian

    Mutation

    R117Q mutation (R95Q according to numbering in Fi…

    Promoter

    CMV

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid

    #218710

    Purpose

    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter

    Depositor

    Bart Nieuwenhuis

    Inserts

    hSaCas9
    Chimeric guide for SaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. 7TFP CDH1 reporter

    Plasmid

    #91704

    Purpose

    lentiviral luciferase reporter containing CDH1 promoter

    Depositor

    Bob Weinberg

    Article

    Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

    Insert

    CDH1 promoter (CDH1 Human)
    

    Use

    Lentiviral and Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    CDH1

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. GPR68-Tango

    Plasmid

    #66371

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR68 (GPR68 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK448 (BromoTag-BSD)

    Plasmid

    #214378

    Purpose

    BromoTag for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BromoTag-BSD
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. ABE8e-Cas9NG

    Plasmid

    #203328

    Purpose

    Plasmid for bacterial purification of codon optimized ABE8e-Cas9NG

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

    Insert

    ABE8e-Cas9NG
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF0372

    Plasmid

    #141593

    Purpose

    Lentiviral vector for overexpressing the NFYB transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFYB (NFYB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-HIF2alpha-P405A/P531A-pcDNA3

    Plasmid

    #18956

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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21. pPRIME-CMV-LNGFR-recipient

    Plasmid

    #11660

    Purpose

    3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling low-affinity nerve growth factor receptor (LNGFR) expression

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    LNGFR

    Expression

    Mammalian

    Available Since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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22. GG-EBNA-OSK2M2L1-PP

    Plasmid

    #102902

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    OSK2M2L1-gRNAs-PGK-puro
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pxpr_502-wt-ltr

    Plasmid

    #182647

    Purpose

    for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    None
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSicoR-eIF1a-GFP-RPL10a

    Plasmid

    #159890

    Purpose

    Expresses GFP-RPL10a for ribosomal immunoprecipitation

    Depositor

    James Ellis

    Article

    Rodrigues et al Cell Rep. 2020 Mar 24;30(12):4179-4196.e

    Insert

    RPL10a (RPL10A Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Promoter

    eIF1a

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pMIK-Neo-mYes

    Plasmid

    #18067

    Depositor

    Marius Sudol

    Article

    Espanel et al J Biol Chem. 2001 Apr 27. 276(17):14514-

    Insert

    yes kinase (Yes1 Mouse)
    

    Expression

    Mammalian

    Available Since

    July 10, 2008

    Availability

    Academic Institutions and Nonprofits only

26. pAAV-Ucp1-empty-miR122

    Plasmid

    #231695

    Purpose

    Empty control vector for pAAV-Ucp1-Cre-miR122

    Depositor

    Joerg Heeren

    Article

    Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

    Type

    Empty backbone

    Use

    AAV

    Promoter

    rat Ucp1 enhancer-promoter

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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27. pcdna3.1 hciap1

    Plasmid

    #8311

    Depositor

    Jon Ashwell

    Article

    Li et al Nature 2002 Mar 21;416(6878):345-7.

    Insert

    cIAP1 (BIRC2 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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28. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

    Plasmid

    #99278

    Purpose

    Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance

    Depositor

    Jordan Miller

    Article

    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

    Inserts

    H2B-mVenus-IRES-mCherry
    Puro
    

    Use

    Lentiviral

    Tags

    H2B, IRES, PGK, mCherry, and mVenus

    Promoter

    EF1 alpha-human and PGK-mouse

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCMV-PE7-SB-NLS

    Plasmid

    #228870

    Purpose

    PE7 connected to MLH1-SB-NLS via 2A sequence

    Depositor

    Sangsu Bae

    Article

    Park et al Cell. 2025 Aug 1:S0092-8674(25)00799-8.

    Insert

    PE7-2A-MLH1-SB-NLS
    

    Use

    CRISPR

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pRK5-HA-Ubiquitin-K27

    Plasmid

    #22902

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K27 only, all other lysines mutated to arginines

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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