223,492 results

1. plk1 WT GFP-myc

   Plasmid

   #132963

   Purpose

   To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

   Depositor

   Michael Yaffe

   Article

   Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

   Insert

   polo-like kinase 1 (PLK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCitrine-N1

   Plasmid

   #54594

   Purpose

   Localization: N1 Cloning Vector, Excitation: 516, Emission: 529

   Depositor

   Oliver Griesbeck, Michael Davidson, Robert Campbell, Roger Tsien

   Article

   Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

   Type

   Empty backbone

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLenti6.2-3xFLAG-V5-ccdB

   Plasmid

   #87072

   Purpose

   Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

   Depositor

   Susan Lindquist, Mikko Taipale

   Article

   Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3xFLAG-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-PGK-hESRRB

   Plasmid

   #60434

   Purpose

   Over-expression of human ESRRB

   Depositor

   Austin Smith

   Article

   Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

   Insert

   ESRRB (ESRRB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWN_U6-TRAC-miniGag-Cas9

   Plasmid

   #228959

   Purpose

   miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

   Depositor

   Jennifer Doudna

   Article

   Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

   Insert

   miniGag-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-SpRY-NLS-6xHis (gbr2101)

   Plasmid

   #181743

   Purpose

   bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   bacterial codon optimized SpRY
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS(SV40)-6xHis and MKIEE MBP tag

   Expression

   Bacterial

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   T7

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRosetta

   Plasmid

   #59700

   Purpose

   Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions.

   Depositor

   John Doench, David Root

   Inserts

   Puro
   BlastR
   EGFP
   

   Use

   Lentiviral

   Tags

   2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-Integrin-Beta1-N-18

   Plasmid

   #55064

   Purpose

   Localization: Integrin/Focal Adhesions, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Integrin-Beta1 (ITGB1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZE27GFP

   Plasmid

   #75452

   Purpose

   Plasmid for constitutive expression of gfp

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   gfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   placIq promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti6.2_mCherry_mito Grx1-roGFP2

    Plasmid

    #155044

    Purpose

    Fluorescent redox reporter

    Depositor

    Vanessa LaPointe

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Lentiviral

    Tags

    mitochondrial targeting sequence and roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. NPY-td-Orange2

    Plasmid

    #83497

    Purpose

    Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

    Depositor

    Sebastian Barg

    Article

    Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

    Insert

    NPY (NPY Human)
    

    Use

    Tags

    tandem mOrange2

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cilantro 2

    Plasmid

    #74450

    Purpose

    Can clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

    Depositor

    Benjamin Ebert

    Article

    Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLIX-hN1ICD

    Plasmid

    #91897

    Purpose

    Doxycycline inducible expression of human Notch1 ICD

    Depositor

    Julien Sage

    Insert

    Notch1 intracellular domain (NOTCH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    codons 1770 to 2555 of human NOTCH1

    Promoter

    TRE promoter, Tet ON

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-sh1368 knockdown c-myc

    Plasmid

    #29435

    Depositor

    Bob Eisenman

    Article

    Lin et al EMBO J. 2009 Oct 21. 28(20):3157-70.

    Insert

    c-Myc shRNA (MYC Human)
    

    Use

    Lentiviral and RNAi

    Tags

    GFP and WPRE

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-Brainbow3.0

    Plasmid

    #45176

    Depositor

    Joshua Sanes

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Insert

    pCAG-Brainbow3.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKL2351

    Plasmid

    #199721

    Purpose

    A pVS1-based T-DNA binary vector and destination vector for Gateway cloning.

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    NPTII
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Anti-GABA-A-R-Pi [N431/64R]

    Plasmid

    #206576

    Purpose

    Mammalian Expression Plasmid of anti-GABA-A-R-Pi (Human). Derived from hybridoma N431/64.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-GABA-A-R-Pi (Homo sapiens) recombinant Mouse monoclonal antibody (GABRP Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hDlx-Flex-dTomato-Fishell_7 (AAV9)

    Viral Prep

    #83894-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hDlx-Flex-dTomato-Fishell_7 (#83894). In addition to the viral particles, you will also receive purified pAAV-hDlx-Flex-dTomato-Fishell_7 plasmid DNA. hDlx-driven, Cre-dependent expression of dTomato. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Promoter

    hDlx

    Tags

    dTomato (Cre-dependent)

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

19. mIFP-TOMM20-N-10

    Plasmid

    #56239

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    NM_014765.2 (TOMM20 Human)
    

    Use

    Tags

    mIFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. lentiGuide-mCherry

    Plasmid

    #217005

    Purpose

    lentiviral plasmid for gRNA cloning with selectable mCherry expression

    Depositor

    Deepta Bhattacharya

    Article

    D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge