We narrowed to 17,342 results for: She

1. pCMV-LambdaN-NbALFA

   Plasmid

   #207627

   Purpose

   Expresses LambdaN-NbALFA (ALFA-tag nanobody) fusion protein in mammalian cells (with a CMV promoter)

   Depositor

   Jay Shendure

   Article

   Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

   Insert

   LambdaN-NbALFA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   pCMV

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSUPER.retro.puro-shNC

   Plasmid

   #113535

   Purpose

   Control retroviral vector.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   shNC (scrambled control shRNA)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFlpStop-HDR-UAS-2.1-tdTom

   Plasmid

   #89148

   Purpose

   FlpStop plasmid for CRISPR/HDR. Has MCS for homology arms

   Depositor

   Tom Clandinin

   Article

   Fisher et al Elife. 2017 Feb 17;6. pii: e22279. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   5XUAS, 3XP3-dsRed

   Available since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SARS-CoV-2 Spike-d18

   Plasmid

   #164583

   Purpose

   A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.

   Depositor

   Jason Sheltzer

   Insert

   SARS-CoV-2 Spike (S SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1256-1273

   Promoter

   EF1a

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRAF-MutL

   Plasmid

   #86607

   Purpose

   E. coli MutL expression plasmid

   Depositor

   Richard Fishel

   Article

   Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

   Insert

   E. coli MutL
   

   Use

   Tags

   his-tag and ald-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRAF-MutH

   Plasmid

   #86609

   Purpose

   E. coli MutH expression plasmid

   Depositor

   Richard Fishel

   Article

   Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

   Insert

   E. coli MutH
   

   Use

   Tags

   his-tag and ald-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRAF-MutS

   Plasmid

   #86605

   Purpose

   E. coli MutS expression plasmid

   Depositor

   Richard Fishel

   Article

   Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

   Insert

   E. coli MutS (D835R,R840E)
   

   Use

   Tags

   his-tag and ald-tag

   Expression

   Bacterial

   Mutation

   D835R, R840E

   Promoter

   T7

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUBC_HA_L7Ae-VP64

   Plasmid

   #68421

   Purpose

   Transient expression of humanized A. fulgidus L7Ae, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   L7Ae
   

   Use

   Synthetic Biology

   Tags

   HA and VP64

   Expression

   Mammalian

   Mutation

   Promoter

   human UbC

   Available since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

   Plasmid

   #201151

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Inserts

   SadCas9-KRAB
   gRNA-TRE
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mutation

   gRNA sequence: ATCAGTGATAGAGAACGTATG

   Promoter

   Available since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBAD-jRCaMP1a

    Plasmid

    #113936

    Purpose

    Bacterial expression of red fluorescent Ca2+ sensor jRCaMP1a

    Depositor

    Thomas Hughes, Robert Campbell

    Article

    Molina et al Biophys J. 2019 May 21;116(10):1873-1886

    Insert

    jRCaMP1a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV_mCherry_2A_HA_2xMS2-VP64

    Plasmid

    #68419

    Purpose

    Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    MS2
    

    Use

    Synthetic Biology

    Tags

    HA and VP64

    Expression

    Mammalian

    Mutation

    MS2: V75E/A81G

    Promoter

    CMV

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ACE2 g1 + Cas9

    Plasmid

    #153011

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + ACE2 gRNA (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBad-HisB-GRvT

    Plasmid

    #87363

    Purpose

    Expresses GRvT, a tandem heterodimer green and red fluorescent protein

    Depositor

    Robert Campbell

    Article

    Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

    Insert

    GRvT
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    AraBad

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBAD-R-GECO1.2

    Plasmid

    #113937

    Purpose

    Bacterial expression of red fluorescent Ca2+ sensor R-GECO1.2

    Depositor

    Thomas Hughes, Robert Campbell

    Article

    Molina et al Biophys J. 2019 May 21;116(10):1873-1886

    Insert

    R-GECO1.2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLJM1-PCYT2

    Plasmid

    #81074

    Purpose

    Expresses PCYT2 in mammalian cells

    Depositor

    Erin O'Shea

    Article

    Chidley et al Elife. 2016 Jul 12;5. pii: e14601. doi:

    Insert

    phosphate cytidylyltransferase 2, ethanolamine (PCYT2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. XW09

    Plasmid

    #65833

    Purpose

    Punc-4c-QS-SL2-mcherry3'UTR-unc-54

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    Neurospora crassa OR74A repressor (qa-1S)
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    Promoter

    Punc-4c

    Available since

    Jan. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTrans_9xD3B_Cluc_Norm

    Plasmid

    #68415

    Purpose

    CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    Cypridina Luciferase-2A-Venus YFP
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    YFP: M153T,V163A,S175G ("Venus") YFP Va…

    Promoter

    minimalCMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ACE2 g2

    Plasmid

    #153012

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

    Depositor

    Jason Sheltzer

    Insert

    ACE2 gRNA (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ACE2 g4 + Cas9

    Plasmid

    #153014

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + ACE2 gRNA (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ERAP1

    Plasmid

    #39174

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ERAP1 (ERAP1 Human)
    

    Use

    Baculovirus expression

    Tags

    TEV-His10-Flag

    Expression

    Mutation

    Promoter

    polyhedrin

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

21. Halo-Sox13

    Plasmid

    #200194

    Purpose

    Expression of full length Sox13

    Depositor

    Sheila Teves

    Article

    Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

    Insert

    Halo-Sox13 (Sox13 Mouse)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF_1alpha

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEFIRES NQO1 WT

    Plasmid

    #61735

    Purpose

    Expresses WT human NQO1

    Depositor

    Yosef Shaul

    Article

    Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    EF1a

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. XW08

    Plasmid

    #65832

    Purpose

    Punc-4-QF-SL2-mcherry-3'UTR-unc-54

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    OR74A quinic acid utilization activator (qa-1F)
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    Promoter

    Punc-4

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLKO-shTRAF2#1

    Plasmid

    #44127

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

25. XW52

    Plasmid

    #65838

    Purpose

    XW52 Pmig-13-Czipper-QF-AD-NLS-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    Czipper-QF-AD-NLS
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    Promoter

    Pmig-13

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. punc-119cR

    Plasmid

    #21596

    Depositor

    Al Fisher

    Article

    Ferguson et al Plasmid. 2009 Jun 8. ():.

    Insert

    unc-119:mCherry (unc-119 Nematode)
    

    Use

    Adds unc-119 marker to amp resistant plasmids

    Tags

    mCherry

    Expression

    Worm

    Mutation

    N/A

    Promoter

    Available since

    Aug. 26, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pWB-TRAF2

    Plasmid

    #44114

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pBad-HisB-GdT

    Plasmid

    #87361

    Purpose

    Expresses GdT, a dimeric green fluorescent protein

    Depositor

    Robert Campbell

    Article

    Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

    Insert

    GdT
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    AraBad

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLKO-shTRAF2#2

    Plasmid

    #44128

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pBAD-O-GECO1

    Plasmid

    #113938

    Purpose

    Bacterial expression of orange fluorescent Ca2+ sensor O-GECO1

    Depositor

    Thomas Hughes, Robert Campbell

    Article

    Molina et al Biophys J. 2019 May 21;116(10):1873-1886

    Insert

    O-GECO1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLenti_9xD3B_Cluc_Norm

    Plasmid

    #68414

    Purpose

    CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," lentiviral format.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    Cypridina Luciferase-2A-Venus YFP
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    YFP: M153T,V163A,S175G ("Venus") YFP Va…

    Promoter

    minimalCMV

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLX-V5-TRAF2-puro

    Plasmid

    #44111

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

33. HOXA9-MSCV-full-GFP

    Plasmid

    #20977

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2008 Jul . 28(14):4609-19

    Insert

    HOXA9 (Hoxa9 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Full length cDNA with ~1.1kb 3'UTR with 2 pu…

    Promoter

    Available since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEN_TmiRc3_shURB5

    Plasmid

    #81066

    Purpose

    Gateway entry vector encoding shRNA to UBR5

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    shRNA targeting UBR5
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBad-HisB-GGvT

    Plasmid

    #87362

    Purpose

    Expresses GGvT, a tandem homodimer green fluorescent protein

    Depositor

    Robert Campbell

    Article

    Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

    Insert

    GGvT
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Neg. Ctrl_sgRNA

    Plasmid

    #111298

    Purpose

    CRISPR KO murine neg. ctrl.

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    NegCtrl sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEN_TmiRc3_shNT

    Plasmid

    #81067

    Purpose

    Gateway entry vector encoding non-targeting shRNA

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    non-targeting shRNA
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. MsCLYBL_sgRNA1

    Plasmid

    #111294

    Purpose

    CRISPR KO murine CLYBL

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    sgRNA1 against murine CLYBL
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. human pirh2-(1-261)

    Plasmid

    #24878

    Depositor

    Cheryl Arrowsmith

    Article

    Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

    Insert

    Pirh2 (RCHY1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

40. His-PaIct

    Plasmid

    #111293

    Purpose

    bacterial expression

    Depositor

    Vamsi Mootha

    Article

    Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

    Insert

    succinyl-CoA:itaconate CoA transferase
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge