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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,225 results for: PAM

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Showing: 1351 - 1400 of 2225 results

  1. pKM5

    Plasmid
    #154166
    Purpose
    Aedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Rel2 (long isoform)
    Tags
    FLAG
    Expression
    Insect
    Promoter
    IE1 promoter and hr5 enhancer from Autographa cal…
    Available Since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154166-plasmid-map-sequence-id-309405

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  2. pKM28

    Plasmid
    #154165
    Purpose
    Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Rel1A
    Tags
    FLAG
    Expression
    Insect
    Promoter
    IE1 promoter and hr5 enhancer from Autographa cal…
    Available Since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154165-plasmid-map-sequence-id-309403

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  3. pAA2

    Plasmid
    #154162
    Purpose
    Firefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Mutation
    16 wt NF-κB sites were replaced with Rel1A-specif…
    Available Since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154162-plasmid-map-sequence-id-309382

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  4. pAA1

    Plasmid
    #154161
    Purpose
    Firefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Mutation
    12 wt NF-κB sites were replaced with Rel1A-specif…
    Available Since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154161-plasmid-map-sequence-id-309344

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  5. pKM62

    Plasmid
    #154160
    Purpose
    Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Mutation
    4 wt NF-κB sites were replaced with Rel2-specific…
    Available Since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154160-plasmid-map-sequence-id-309343

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  6. pKM59

    Plasmid
    #154158
    Purpose
    Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak sequence of the Metchnikowin gene and additional NF-κB site.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Promoter
    Enhancer for the Drosophila melanogaster Metchnik…
    Available Since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154158-plasmid-map-sequence-id-309332

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  7. pKM52

    Plasmid
    #154155
    Purpose
    Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel2 NF-κB site
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Promoter
    Drosophila melanogaster minimal Hsp70 promoter wi…
    Available Since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154155-plasmid-map-sequence-id-309312

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  8. pKM51

    Plasmid
    #154154
    Purpose
    Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel1A NF-κB site
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Firefly luciferase
    Use
    Luciferase
    Promoter
    Drosophila melanogaster minimal Hsp70 promoter wi…
    Available Since
    Aug. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154154-plasmid-map-sequence-id-309306

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  13. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139987-plasmid-map-sequence-id-275335

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  37. pKM33

    Plasmid
    #154167
    Purpose
    Aedes aegypti Rel2 (long isoform) with C-terminal IκB domain deleted, expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    Rel2 (long isoform)
    Tags
    FLAG
    Expression
    Insect
    Mutation
    C-terminal IκB domain deleted
    Promoter
    IE1 promoter and hr5 enhancer from Autographa cal…
    Available Since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154167-plasmid-map-sequence-id-309408

    Enlarge

  40. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid
    #140001
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140001-plasmid-map-sequence-id-477414

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  42. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid
    #139998
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139998-plasmid-map-sequence-id-394975

    Enlarge

  48. pCMV-T7-SpCas9-VRQR-P2A-EGFP (RTW3161)

    Plasmid
    #139992
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139992-plasmid-map-sequence-id-275524

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Showing: 1351 - 1400 of 2225 results