We narrowed to 10,414 results for: 158

1. pcDNA3-nuc-GsCT

   Plasmid

   #205517

   Purpose

   Nuclear-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   nuc-GsCT (GNAS Synthetic, Human)
   

   Use

   Tags

   Histone H2A and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p2T-CMV-ABE8e_dNRCH-BlastR

   Plasmid

   #232720

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

   Insert

   ABE8e-dCas9(NRCH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   within Cas9 D10A H840A

   Promoter

   Available since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mEos3.2FPP

   Plasmid

   #203758

   Purpose

   Encodes the membrane anchor of HRas, including the last 10 residues of the C terminus with 1 farnesylation and 2 palmitoylations, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   HRas c-term (HRAS Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP

   Plasmid

   #189630

   Purpose

   Expresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations.

   Depositor

   Jerzy Szablowski

   Article

   Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Inserts

   Gaussia luciferase fused to Fc
   IRES-EGFP
   

   Use

   AAV, Cre/Lox, and Luciferase

   Tags

   Gluc and IgG1 Fc

   Expression

   Mammalian

   Mutation

   Promoter

   IRES and hSyn

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNQL006-NANOG-FKBPV-HA2-P2A-eGFP targeting construct

   Plasmid

   #175554

   Purpose

   Targeting vector to introduce an FKBPV-HA2-P2A-eGFP cassette at the mouse NANOG locus. Degradation Tag (dTAG) system.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   NANOG
   

   Use

   Mouse Targeting

   Tags

   FKBPV-HA2-P2A-eGFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRetroX SgK269 WT

   Plasmid

   #68028

   Purpose

   expression of Sgk269 in mammalian cells

   Depositor

   Roger Daly

   Article

   Croucher et al Cancer Res. 2013 Mar 15;73(6):1969-80. d

   Insert

   PEAK1 (PEAK1 Human)
   

   Use

   Retroviral

   Tags

   FLAG/myc, HA, and IRES-DsRed

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

7. RCAS-Flag-BRAFV600E

   Plasmid

   #126615

   Purpose

   Retroviral expression of Flag tagged human oncogenic BRAFV600E

   Depositor

   Yi Li

   Article

   Bu et al Cancer Res. 2019 Jan 1;79(1):61-71. doi:

   Insert

   BRAF (BRAF Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   V600E

   Promoter

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCW57.1-Eomes-3xT7

   Plasmid

   #200888

   Purpose

   Inducible lentiviral expression of Eomes

   Depositor

   Kai Ge

   Article

   Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

   Insert

   Eomes (Eomes Mouse)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   3xT7

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet ON

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA

   Plasmid

   #210501

   Purpose

   expresses LAUNCHER component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJT216 Lenti pEF-dCas9-3XFLAG-LibCloneSite-T2A-tagBFP-P2A-Blast

    Plasmid

    #187320

    Purpose

    dCas9 expression vector with cloning site for C terminal fusions

    Depositor

    Lacra Bintu, Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. lentiCRISPRv2_sgCtrl#1

    Plasmid

    #174148

    Purpose

    Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKHH030_sgCtrl#1

    Plasmid

    #174142

    Purpose

    Lentiviral vector expressing a control sgRNA that targets a safe harbor site

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hU6-pegRNA-EF-1α-Cre Template

    Plasmid

    #196036

    Purpose

    The template UPEC vector designed for cloning of a single pegRNA and subsequent co-expression with Cre.

    Depositor

    Tyler Jacks

    Article

    Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 and EF-1-alpha

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR223_TRAF5_WT

    Plasmid

    #82167

    Purpose

    Gateway Donor vector containing TRAF5 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    TRAF5 (TRAF5 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223_NMNAT3_WT

    Plasmid

    #81885

    Purpose

    Gateway Donor vector containing NMNAT3 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    NMNAT3 (NMNAT3 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AICSDP-34: TNNI1-mEGFP

    Plasmid

    #114411

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Polymerase-unfused click editor - pCMV-T7-PCV2-nCas9 (LM2702)

    Plasmid

    #208961

    Purpose

    Polymerase-unfused click editor comprised of PCV2 fused to nCas9, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDY0738 pCAG-NLS-SscINT

    Plasmid

    #179113

    Purpose

    SscINT expression vector

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCAG-NLS-SscINT
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDY0739 pCAG-NLS-SacINT

    Plasmid

    #179114

    Purpose

    SacINT expression vector

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCAG-NLS-SacINT
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMP71-hPDL-1

    Plasmid

    #118631

    Purpose

    human PDL-1 coding sequence subcloned into pMP71 retroviral plasmid

    Depositor

    Sébastien Walchli

    Article

    Josefsson et al Cancer Immunol Res. 2019 Mar;7(3):355-36

    Insert

    hPDL-1 (CD274 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDONR223-ZNF655

    Plasmid

    #88578

    Purpose

    Donor Vector containing ZNF655 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF655 (ZNF655 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDONR221-ZNF736

    Plasmid

    #101602

    Purpose

    Donor Vector containing ZNF736 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF736 (ZNF736 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-HA-CCND1

    Plasmid

    #172649

    Purpose

    Expresses HA-tagged cyclin D1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Nickase SpCas9 - pCMV-T7-nSpCas9(H840A)-P2A-GFP (HES140)

    Plasmid

    #208976

    Purpose

    Nickase SpCas9 (H840A) with a P2A GFP and 3xFLAG, expressed from a CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    nSpCas9(H840)-BPNLS-P2A-GFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti_EF1α-NES-Kinprola_PKA-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid

    #233371

    Purpose

    EF1α driven co-expression of the PKA activity recorder Kinprola_PKA fused to mEGFP and Cas9 expression through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Inserts

    NES-Kinprola_PKA-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    

    Use

    Lentiviral

    Tags

    FLAG, NES, and mEGFP

    Expression

    Mutation

    Promoter

    EF1α

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDY0312 PhiBT Integrase

    Plasmid

    #179111

    Purpose

    PhiBT Integrase expression vector

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    PhiBT Integrase
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUt-mPF-GFP

    Plasmid

    #199222

    Purpose

    LPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    rtTA-Advanced::P2A::EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTagGFP2-N SIN1

    Plasmid

    #124919

    Purpose

    For expression of wild-type SIN1-GFP

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MAPKAP1 (MAPKAP1 Human)
    

    Use

    Tags

    TagGFP2

    Expression

    Mammalian

    Mutation

    Insert ORF was sirently mutated to be resistent t…

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-HA-CDK2

    Plasmid

    #172613

    Purpose

    Expresses HA-tagged CDK2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK2 (CDK2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Gfp-zf-vasa

    Plasmid

    #167323

    Purpose

    Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.

    Depositor

    Goro Yoshizaki

    Article

    Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

    Insert

    zf vasa 3'UTRs (ddx4 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge