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Plasmid#75577Purpose3rd generation lentiviral gRNA plasmid targeting human NUAK1DepositorInsertNUAK1 (NUAK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE
Plasmid#138143PurposeExpresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caacDepositorInsertPguCas13b
UseLentiviralTagsNLSExpressionMutationPromoterAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_CD WT TET1
Plasmid#124082PurposeExpression of wild-type catalytic domain only form of TET1DepositorInsertTET1 catalytic domain (TET1 Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterEF1 alphaAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
non-targeting control gRNA (BRDN0001145598)
Plasmid#80173Purpose3rd generation lentiviral gRNA plasmid, non-targeting control gRNADepositorInsertNon-targeting control gRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTol2-10xUAS-Voltron
Plasmid#119039PurposeUAS-driven expresion of Voltron in zebrafishDepositorInsertVoltron
UseZebrafish expressionTagsExpressionMutationPromoterE1bAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
NUAK1 gRNA (BRDN0001147733)
Plasmid#75576Purpose3rd generation lentiviral gRNA plasmid targeting human NUAK1DepositorInsertNUAK1 (NUAK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
NUAK1 gRNA (BRDN0001162525)
Plasmid#75578Purpose3rd generation lentiviral gRNA plasmid targeting human NUAK1DepositorInsertNUAK1 (NUAK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTol2-10xUAS-Voltron-ST
Plasmid#119040PurposeUAS-driven expresion of soma-localized Voltron in zebrafishDepositorInsertVoltron-ST
UseZebrafish expressionTagsExpressionMutationPromoterE1bAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRE3G-BE3RA-PGK-Puro
Plasmid#110846PurposeLentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)DepositorInsertBE3RA
UseLentiviralTagsExpressionMutationD10APromoterTRE3GAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS5-YFP-NLS-mut
Plasmid#120832PurposeEctopic expression of ZIKV proteinDepositorInsertZIKV NS5-eYFP "NLS-mut"
UseTagseYFP-V5ExpressionMammalianMutationR372A/Q373A/K387A/H388A/K389APromoterCMVAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMonAID_nCas9-PmCDA_Hyg_ALS
Plasmid#91693PurposeMonocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAlsDepositorInsertSpCas9
UseTagsPmCDA1ExpressionPlantMutationD10A for nickase Cas9Promoter2x35SAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
SARS_COV2_ORF3a_HA_BASU
Plasmid#153994PurposeExpress Orf3a gene along with HA and BASUDepositorInsertOrf3a (ORF3a Severe acute respiratory syndrome coronavirus 2)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
NSK026 CMV-TO-p53R248W (FLP-IN)
Plasmid#191168PurposeExpression of human p53 mutant R248W in mammalian cellsDepositorInsertp53(R248W) (TP53 Human)
UseTagsExpressionMammalianMutationR248WPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PHKG1 gRNA (BRDN0001144981)
Plasmid#76356Purpose3rd generation lentiviral gRNA plasmid targeting human PHKG1DepositorInsertPHKG1 (PHKG1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP
Plasmid#153071PurposeLentiviral vector expressing human ZC3H12D mutated (D95N)DepositorInsertMutated ZC3H12D (D95N) (ZC3H12D Human)
UseLentiviralTagsExpressionMammalianMutationChanged D95 to N, known to abrogate the RNase enz…PromoterEF1αAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
K15-EGFP
Plasmid#44266DepositorInsertK15 promoter (-4.8) (Krt15 Mouse)
UseMouse Targeting; Fluorescence microscopyTagsEGFPExpressionMammalianMutationContains the murine K15 promoter (-4.8) fragmentPromotermurine K15 promoter (-4.8) fragmentAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSF-Ivy-his-hLYZ
Plasmid#83920PurposeCo-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.DepositorInsertsUseTagsIvy protein has C-terminal hisx6 tagExpressionBacterialMutationIvy leader sequence deleted and replaced.PromoterT7 and t7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-BE3-Y130F
Plasmid#113428PurposeExpresses hA3A-BE3-Y130F in mammalian cellsDepositorInserthA3A-BE3-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
UseCRISPRTagsExpressionMammalianMutationhAPOBEC3A_Y130FPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP22-5'UTR-BBa_B0034
Plasmid#216626PurposeNanoLuc reporter plasmid to optimize chloroplast cell-free systems with RBS BBa_B0034 in 5'UTRDepositorInsertT7p-5'B0034-NanoLuc-3'TMV
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOP01-UTC-7.0
Plasmid#216625PurposeNanoLuc reporter plasmid to optimize chloroplast cell-free systems, universal test constructDepositorInsertT7p-5'g10-NanoLuc-3'TMV
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
PKN3 gRNA (BRDN0001146586)
Plasmid#77947Purpose3rd generation lentiviral gRNA plasmid targeting human PKN3DepositorInsertPKN3 (PKN3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_CD MUT TET1
Plasmid#124083PurposeExpression of mutated catalytic domain only form of TET1DepositorInsertTET1 - catalytic domain mutated (TET1 Human)
UseTagsFlag and HAExpressionMammalianMutationH1672Y, D1674APromoterEF1 alphaAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.PAC
Plasmid#57829PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-PAC
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCpf1-BE-YEE
Plasmid#107687PurposeExpresses dLbCpf1-BE-YEE in mammalian cellsDepositorInsertdLbCpf1-BE-YEE
UseTagsExpressionMammalianMutationAPOBEC1(W90Y/R126E/R132E), dLbCpf1(D832A/E925A/D1…PromoterCMVAvailable sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
STK17A gRNA (BRDN0001149480)
Plasmid#77916Purpose3rd generation lentiviral gRNA plasmid targeting human STK17ADepositorInsertSTK17A (STK17A Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
STK17B gRNA (BRDN0001146764)
Plasmid#76798Purpose3rd generation lentiviral gRNA plasmid targeting human STK17BDepositorInsertSTK17B (STK17B Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PFKFB4 gRNA (BRDN0001149495)
Plasmid#77123Purpose3rd generation lentiviral gRNA plasmid targeting human PFKFB4DepositorInsertPFKFB4 (PFKFB4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK322 (TOP2A-mAC (Hygro))
Plasmid#140653PurposeTOP2A tagging with mAID-CloverDepositorInsertTOP2A (TOP2A Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
PKN3 gRNA (BRDN0001146792)
Plasmid#77948Purpose3rd generation lentiviral gRNA plasmid targeting human PKN3DepositorInsertPKN3 (PKN3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PKN3 gRNA (BRDN0001146747)
Plasmid#77949Purpose3rd generation lentiviral gRNA plasmid targeting human PKN3DepositorInsertPKN3 (PKN3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only