163,421 results

1. pAG148 FRB-NFAST

   Plasmid

   #130812

   Purpose

   Expresses FRB-NFAST in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

   Insert

   FRB-NFAST
   

   Use

   Tags

   myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-mTH-GFP

   Plasmid

   #99128

   Purpose

   An AAV genome that expresses GFP from the mouse TH promoter

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mTH

   Available since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD33-Gm

   Plasmid

   #65098

   Purpose

   bacterial expression vector with Gentamycin selection, based on pBAD33

   Depositor

   Juan Tomas

   Article

   Jimenez et al J Bacteriol. 2009 Apr;191(7):2228-36. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-EF1A-CD19.28H.28TM.28.3z

   Plasmid

   #200678

   Purpose

   Modular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD28HD-CD28TM-CD28-CD3z-P2A-EGFP (CD28, CD247 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. 35S-VP16-GWY/pAM

   Plasmid

   #201234

   Purpose

   Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain

   Depositor

   Michael Ayliffe

   Article

   Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

   Insert

   35S-VP16-GWY
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. VAMP2-pHmScarlet

   Plasmid

   #166890

   Purpose

   For vesicle exocytosis visualization using pHmScarlet

   Depositor

   Pingyong Xu

   Article

   Liu et al Nat Commun. 2021 Mar 3;12(1):1413. doi:

   Insert

   VAMP2 (Vamp2 Mouse)
   

   Use

   Tags

   pHmScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Human SAM library in lentiSAMv2 backbone

   Pooled Library

   #1000000078

   Purpose

   Genome-wide CRISPR gRNA pooled library for activation of human genes

   Depositor

   Feng Zhang

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

8. mTurquoise2

   Plasmid

   #198196

   Purpose

   Spectral correction: Expression of mTurquoise2

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A206K

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-HA-mRipk3

   Plasmid

   #78805

   Purpose

   To overexpress mRipk3 in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK3 (Ripk3 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. 35S-GAL4BD-GWY/pAM

    Plasmid

    #201233

    Purpose

    Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    35S-GAL4BD-GWY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-6xHis-(-30)GFP

    Plasmid

    #62936

    Purpose

    Expression of 6xHis-(-30)GFP in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (-30)GFP
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-6XHis-human SUMO 2

    Plasmid

    #133771

    Purpose

    express 6Xhis- SUMO 2 protein

    Depositor

    Martine Roussel

    Article

    Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    SUMO 2 (SUMO2 Human)
    

    Use

    Tags

    6XHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV Retrograde)

    Viral Prep

    #154867-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ulrik Gether

    Article

    Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03

    Promoter

    Human Synapsin-1

    Tags

    mCherry (Flp-dependent)

    Available since

    Oct. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-Sst44-GFP

    Plasmid

    #172310

    Purpose

    SST interneuron-restricted gene regulatory element GRE44, to drive GFP expression in SST+ interneurons

    Depositor

    Michael Greenberg

    Article

    Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

    Insert

    Sst44
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pB-Globin

    Available since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUCmini-iCAP-AAV9-X1.1

    Plasmid

    #196836

    Purpose

    non-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

    Insert

    Synthetic construct isolate AAV9-X1.1 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 (AAV1)

    Viral Prep

    #172922-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 (#172922). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3 plasmid DNA. Syn-driven expression of calcium sensor jGCaMP8m in axons with cytosolic expression of mRuby3. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Promoter

    Syn

    Tags

    mRuby3

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

17. AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR

    Plasmid

    #171594

    Purpose

    Strong human ACE2 abundance from Bxb1 landing pad

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Inserts

    ACE2 (ACE2 Human)
    mCherry
    H2A
    Pac
    

    Use

    Synthetic Biology; Promoterless bxb1 dna recombin…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti-mito-mTagBFP2

    Plasmid

    #216130

    Purpose

    Lentiviral vector for expressing mTagBFP2 localizing to mitochondria.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCS2-nCas9n

    Plasmid

    #47929

    Purpose

    expression of an optimized Cas9 for genome-editing in zebrafish

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    nls-zcas9-nls
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCh-Climp63

    Plasmid

    #136293

    Purpose

    expression of Climp63

    Depositor

    Gia Voeltz

    Article

    Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

    Insert

    Cytoskeletal-linking protein 63 (Ckap4 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSGDlucV3.0

    Plasmid

    #119760

    Purpose

    dual luciferase with FMDV StopGo sequences flanking MCS

    Depositor

    John Atkins

    Article

    Loughran et al Mol Cell. 2022 Oct 6;82(19):3745-3749.e2

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 and T7

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. HA-HIF1alpha P402A/P564A-pcDNA3

    Plasmid

    #18955

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 402 to Alanin…

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSBbi-Neo

    Plasmid

    #60525

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-SHP2 WT

    Plasmid

    #8381

    Depositor

    Ben Neel

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GFP-Mfn1

    Plasmid

    #141154

    Purpose

    Mammalian expression and labeling mitofusin 1

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    Mitofusin 1 (MFN1 Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTP943

    Plasmid

    #104157

    Purpose

    Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse IgG1 Fab specific nanobody TP886
    

    Use

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pU6-hROSA26-1-1kbdonor

    Plasmid

    #234736

    Purpose

    PseCAST crRNA targeting hROSA26-1, with 1 kb donor transposon

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    hROSA26-1 crRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pETDuet S2

    Plasmid

    #171921

    Purpose

    Express RNA-based (p)ppGpp sensor and a NirFP

    Depositor

    Mingxu You

    Article

    Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

    Inserts

    RNA-based (p)ppGpp sensor
    NirFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ER-mNeonGreen

    Plasmid

    #137804

    Purpose

    Visualization of the Endoplasmic Reticulum

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMega-MaPylRS

    Plasmid

    #200225

    Purpose

    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.

    Depositor

    Alanna Schepartz

    Article

    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

    Inserts

    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    proK-lacO and tacI

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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