176,067 results
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Plasmid#26852DepositorInsertLoxP-3xPDsRed-LoxP
ExpressionInsectAvailable SinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
PBCAG-eGFP
Plasmid#40973DepositorInsertsPiggyBac transposon 3' terminal repeats
PiggyBac 5' terminal repeats
ExpressionMammalianAvailable SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry
Plasmid#188766PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterEF1aAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (AAV1)
Viral Prep#100853-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (#100853). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven, Cre-dependent GECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
His6-TEV-CRBN-midi
Plasmid#215330PurposeCRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader designDepositorInsertCRBN (CRBN Human)
Tags6xHis and TEVExpressionBacterialMutationcontains amino acids 41-187 followed by a Gly-Ser…PromoterlacIAvailable SinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#42876PurposeBacterial expression of Cas9 nuclease, tracrRNA and crRNA guideDepositorInserttracr/Cas9
UseCRISPR; E.coliExpressionBacterialAvailable SinceFeb. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Cre (AAV Retrograde)
Viral Prep#55636-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-EF1a-Cre (#55636). In addition to the viral particles, you will also receive purified pAAV-EF1a-Cre plasmid DNA. EF1a-driven expression of Cre recombinase. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-DTR-GFP (AAV2)
Viral Prep#124364-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-FLEX-DTR-GFP (#124364). In addition to the viral particles, you will also receive purified pAAV-FLEX-DTR-GFP plasmid DNA. CBA-driven, Cre-dependent expression of DTR-GFP. These AAV preparations are suitable purity for injection into animals.DepositorPromoterChicken B-actinTagsGFP (Cre-dependent)Available SinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro
Plasmid#188768PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterSFFVAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYL192 (TRV1)
Plasmid#148968PurposeTobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)DepositorInsertTRV RNA1
UsePlant vector with trv rna1; this vector used with…MutationP697S mutation in putative replicase. S239G and S…Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cre-P2A-dTomato (AAV Retrograde)
Viral Prep#107738-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsdTomato (physically separate, not a fusion protein)Available SinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-mCherry-T2A-Puro
Plasmid#72264PurposeExpress mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.DepositorInsertmCherry-T2A-Puro
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
attB-puro donor.dna
Plasmid#181923PurposeattB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertionDepositorInsertattB-EGFP-EF1α-PuroR-T2A-TagBFP
ExpressionMammalianAvailable SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-EGFP (AAV Retrograde)
Viral Prep#50457-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-DIO-EGFP (#50457). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-EGFP plasmid DNA. hSyn-driven, Cre-dependent EGFP expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFP (Cre-dependent)Available SinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human sgRNA library Brunello in lentiCRISPRv2
Pooled Library#73179PurposeHuman sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controlsDepositorHas ServiceLentiviral PrepExpressionMammalianUseCRISPR and LentiviralAvailable SinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Neo DEST (w531-1)
Plasmid#19067Purpose3rd gen lentiviral Gateway destination vector, expression, PGK promoter, NeoDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-ASCL1-DLX2
Plasmid#182307PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expressionTagsNone and T2A-mycNLS-mTagBFP2ExpressionMammalianPromoterEF1a and pTRE3G-bidirectionalAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hChR2(H134R)-mCherry (AAV9)
Viral Prep#26976-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-hChR2(H134R)-mCherry (#26976). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-mCherry plasmid DNA. Synapsin-driven, humanized channelrhodopsin H134R mutant, fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherryAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV2)
Viral Prep#44362-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven, Cre-dependent, hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available SinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2
Plasmid#41843DepositorInsertCD20
TagsHA and c-mycExpressionYeastAvailable SinceJan. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM4D(Gi)-mCherry (AAV5)
Viral Prep#44362-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-hSyn-DIO-hM4D(Gi)-mCherry (#44362). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM4D(Gi)-mCherry plasmid DNA. Syn-driven, Cre-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal silencing. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCherry (Cre-dependent)Available SinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV GFP1-9
Plasmid#182244PurposeTripartite split-GFP mammalian. Express GFP1-9 detector fragmentDepositorInsertGFP 1-9 OPT
ExpressionMammalianPromoterCMVAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-GFP1-10-IRES-Puro
Plasmid#211467PurposeLentivirus backbone expressing GFP1-10DepositorInsertGFP1-10
UseLentiviralExpressionMammalianPromoterEF-1aAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV8)
Viral Prep#154867-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterHuman Synapsin-1TagsmCherry (Flp-dependent)Available SinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-Guide-Puro
Plasmid#86708PurposeMakes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.DepositorInsertEF1a-Puro-WPRE-hU6-gRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCF159_BRL
Plasmid#225962PurposeCMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP production.DepositorInsertCMV-Intron-BRL (env protein)
ExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-tdTomato (AAV9)
Viral Prep#28306-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (Cre-dependent)Available SinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Alive
Plasmid#20860PurposeAlive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.DepositorInsertStreptavidin-Alive
TagsHisExpressionBacterialMutationWt geneAvailable SinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
lenti-EF1a-dCas9-VPR-Puro
Plasmid#99373Purpose3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)DepositorInsert(Sp)dCas9-VPR-P2A-Puro
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1aAvailable SinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-mitoDsRed
Plasmid#44386Purpose3rd generation lentiviral vectorDepositorInsertmitoDsRed
UseLentiviralTags61 aa targeting sequence of the P1 isoform F1F0-A…ExpressionMammalianPromoterCMVAvailable SinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only