164,884 results

1. FgH1tUTG

   Plasmid

   #70183

   Purpose

   Inducible expression of guide RNA with fluorescent GFP reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   H1-Tet-sgrna cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EGFP-Ubiquitin

   Plasmid

   #187910

   Purpose

   Mammalian cell expression of EGFP-tagged Ubiquitin

   Depositor

   Erika Holzbaur

   Article

   Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

   Insert

   UBB (UBB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pM162

   Plasmid

   #173221

   Purpose

   Expresses ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP(1-9)-GB1 and Puro-T2A-MCP-mCherry-GFP11-GB1

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Inserts

   ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP (1-10)-GB1
   MCP-mCherry-GFP11-GB1
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. VEGFR2-mCh

   Plasmid

   #108854

   Purpose

   Encodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker

   Depositor

   Kalina Hristova

   Article

   Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

   Insert

   KDR (KDR Human)
   

   Use

   Tags

   Labeled with mCherry on the C-Terminus via a 3 am…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLVX-Flag-CDK8-WT-IRES-mCherry

   Plasmid

   #68856

   Purpose

   Expresses Human CDK8 Wild-Type

   Depositor

   Matthew Shair

   Article

   Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

   Insert

   cyclin-dependent kinase 8 (CDK8 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 7a sgRNA for EJ7-GFP and 4-μHOM reporters

   Plasmid

   #113620

   Purpose

   sgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reporters

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7a sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pDAI-SceI-pheS

   Plasmid

   #111611

   Purpose

   Yeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.

   Depositor

   Tim Tolker-Nielsen

   Article

   Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

   Insert

   pheS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CMV-H2B-mScarlet-I

   Plasmid

   #182297

   Purpose

   Localization of mScarlet-I fluorescent protein to histone 2B

   Depositor

   Thierry Fournier, Severine Degrelle

   Article

   Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

   Insert

   mScarlet-I
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHR_Gal4UAS_tBFP_PGK_mCitrine

   Plasmid

   #188386

   Purpose

   Lentiviral vector - tBFP reporter for Gal4-based SNIPRs with a constitutive mCitrine;

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   Gal4UAS_tBFP_PGK_mCitrine
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAcGFP-N1_CBP

    Plasmid

    #221903

    Purpose

    Transient expression of GFP-tagged CBP in mammalian cells

    Depositor

    Daniel Bose

    Insert

    CBP (CREBBP Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pQE30-His-T7RNAP

    Plasmid

    #124138

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    T7 RNA polymerase (T7p07 Enterobacteria phage T7)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHR_SFFV_LaG17_synNotch_TetRVP64

    Plasmid

    #79128

    Purpose

    Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch TetRVP64 Receptor (SFFV promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    LaG17 GFP nanobody synNotch TetRVP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX330A-FBL/PITCh

    Plasmid

    #63671

    Purpose

    Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pLX302_FOXA1-V5

    Plasmid

    #70090

    Purpose

    Expression of human FOXA1 with C-terminal V5 tag, puromycin selection marker, 3rd generation lentiviral vector

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    FOXA1 (FOXA1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP-Nucleolin

    Plasmid

    #28176

    Depositor

    Michael Kastan

    Article

    Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

    Insert

    Nucleolin (NCL Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ATGL*-V5-APEX2

    Plasmid

    #170574

    Purpose

    A C-terminal APEX2 fusion to the human ATGL S47A protein

    Depositor

    James Olzmann

    Article

    Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

    Insert

    ATGL* (PNPLA2 Human)
    

    Use

    Lentiviral

    Tags

    V5-APEX2

    Expression

    Mammalian

    Mutation

    changed serine 47 to alanine

    Promoter

    CMV

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Cerulean

    Plasmid

    #15214

    Depositor

    Dave Piston

    Article

    Rizzo et al Nat Biotechnol. 2004 Apr . 22(4):445-9.

    Insert

    monomeric Cerulean
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K

    Promoter

    Available since

    June 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRosetta

    Plasmid

    #59700

    Purpose

    Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions.

    Depositor

    David Root, John Doench

    Inserts

    Puro
    BlastR
    EGFP
    

    Use

    Lentiviral

    Tags

    2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5_mCherry_P2AT2A_GFP-nb-2xKS

    Plasmid

    #238236

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb-2xKS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-2xKS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF0123

    Plasmid

    #141528

    Purpose

    Lentiviral vector for overexpressing the OLIG2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    OLIG2 (OLIG2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge