162,050 results

1. pLminP_Luc2P_RE17

   Plasmid

   #90359

   Purpose

   NFAT - PKC/Ca++ gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   NFAT

   Available since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-p53-shRNA-427

   Plasmid

   #25636

   Depositor

   Todd Waldman

   Article

   Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

   Insert

   p53-shRNA-427 (TP53 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (AAV5)

   Viral Prep

   #198511-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (#198511). In addition to the viral particles, you will also receive purified pAAV_hSyn-DIO-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven Cre-dependent expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Promoter

   Syn

   Tags

   mScarlet (Cre-dependent)

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-myc-CUL3

   Plasmid

   #19893

   Depositor

   Yue Xiong

   Article

   Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

   Insert

   cullin 3 (CUL3 Human)
   

   Use

   Tags

   3xmyc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV hSyn GFP-Cre

   Plasmid

   #175381

   Purpose

   expresses GFP-Cre under the control of a human synapsin promoter

   Depositor

   Jason Aoto

   Insert

   GFP-Cre recombinase
   

   Use

   AAV

   Tags

   Cre is fused to GFP. GFP is fused to additional n…

   Expression

   Mutation

   Promoter

   human synapsin

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-link-ymScarletI-URA3

   Plasmid

   #168055

   Purpose

   Replaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymScarletI
   

   Use

   Tags

   ymScarletI

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX neo PFKP-GFP

   Plasmid

   #116940

   Purpose

   Expression of PFKP-GFP fusion protein

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   PFKP (PFKP Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Str-Golgin84_VSVG-SBP-EGFP

   Plasmid

   #65305

   Purpose

   synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Golgin-84 (GOLGA5 Human)
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. aeBlue chromoprotein

   Plasmid

   #117846

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, aeBlue
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT3-EF1aH NICD1

    Plasmid

    #86500

    Purpose

    Mammalian expression of Myc tagged NICD1

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain only (AA 1753-2531)

    Promoter

    EF1a

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. 478 p5Efli1ep

    Plasmid

    #31160

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    chimeric fli1ep enhancer/promoter fragment
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-RIGI-Apex- T2A-EGFP

    Plasmid

    #167288

    Purpose

    Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    APEX2 and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_SLC1A2

    Plasmid

    #131901

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC1A2 (SLC1A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. hCRISPRi-v2, genome-wide, top 5 sgRNAs/gene

    Pooled Library

    #83969

    Purpose

    Version 2 of the human CRISPRi genome wide library

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Use

    Lentiviral

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pGP-CMV-NES-jRGECO1a

    Plasmid

    #61563

    Purpose

    Red fluorescent calcium sensor protein

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    NES-jRGECO1a
    

    Use

    Tags

    nuclear export signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. FRESCA-syntide

    Plasmid

    #132415

    Purpose

    FRET-based sensor for CaMKII activity

    Depositor

    Margaret Stratton

    Article

    Ardestani et al J Biol Chem. 2019 Jun 14. pii: RA119.009

    Insert

    FRESCA-syntide
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDIV643

    Plasmid

    #226182

    Purpose

    Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

    Insert

    uidA marker flanked with targeting regions to KU70 gene in K.phaffii
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TEF1p

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCap-SG5-Apr

    Plasmid

    #227579

    Purpose

    Capture plasmid with apramycin resistance and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSFFV_Gal4-VP64-IRES-mCherry (pLZA139)

    Plasmid

    #203909

    Purpose

    Encodes wild type Gal4-VP64 transcription factor in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    Gal4-VP64-IRES-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. mEGFP-pBAD

    Plasmid

    #54622

    Purpose

    Localization: Protein Expression Vector , Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis and mEGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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