163,636 results

1. AAV-P(Cry1)-forward-intron336-Venus-NLS-D2

   Plasmid

   #110054

   Purpose

   Cry1 transcription fluorescence reporter

   Depositor

   Eric Erquan Zhang

   Article

   Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

   Insert

   Cry1 promoter and Venus (Cry1 Mouse)
   

   Use

   AAV

   Tags

   NLS-D2

   Expression

   Mammalian

   Mutation

   Promoter

   Cry1

   Available since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. plenti-CAG-gate-FLAG-IRES-GFP

   Plasmid

   #107398

   Purpose

   Lentiviral gateway destination vector

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWALIUM10-GW-TurboID-V5

   Plasmid

   #196283

   Purpose

   Gateway destination vector to express C-terminal TurboID fusion under UAS promoter, for making transgenic flies

   Depositor

   Norbert Perrimon

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. L4440_BioBrick-SCR-sgRNA-B

   Plasmid

   #177812

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Rlucrarr3-Flash2

   Plasmid

   #74130

   Purpose

   FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.

   Depositor

   Louis Luttrell

   Article

   Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

   Insert

   Arrestin3
   

   Use

   Tags

   Renilla luciferase (rLuc)

   Expression

   Mammalian

   Mutation

   Insertion of FlAsH motif (CCPGCC) following amino…

   Promoter

   Available since

   April 6, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pAW13.lentiguide.mCherry

   Plasmid

   #104375

   Purpose

   mCherry vector for cloning in guides

   Depositor

   Richard Young

   Article

   Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-HSF1

   Plasmid

   #32537

   Depositor

   Stuart Calderwood

   Article

   Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

   Insert

   HSF1 (HSF1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   see depositor comments below

   Promoter

   CMV

   Available since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBad-sfGFP 150TAG

   Plasmid

   #85483

   Purpose

   Arabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

   Insert

   superfolder GFP 150TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   150TAG (amino acid 150 mutated to stop codon)

   Promoter

   Arabinose

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIRE4-Azi

   Plasmid

   #105829

   Purpose

   Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells

   Depositor

   Irene Coin

   Article

   Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.

   Inserts

   Humanized EAziRS
   4 copies of the U6-BstYam tRNA cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   (Y37L, D182S, F183M, L186A, D265R)TyrRS

   Promoter

   PGK and U6

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPAmCherry-a-tubulin

    Plasmid

    #31930

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Use

    Tags

    a-tubulin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ef1a-Coff/Fon-mCherry

    Plasmid

    #137134

    Purpose

    Intersectional viral expression of mCherry in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Human SLC KO Library

    Pooled Library

    #132552

    Purpose

    Expresses gRNAs for knockout of the human Solute Carrier family

    Depositor

    Giulio Superti-Furga

    Article

    Girardi et al Nat Chem Biol. 2020 Apr;16(4):469-478. d

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3-PRB

    Plasmid

    #89130

    Purpose

    mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418

    Depositor

    Elizabeth Wilson

    Article

    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

    Insert

    human progesterone receptor-B (PGR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. modRNAc1-Cas9-2A-puro

    Plasmid

    #216390

    Purpose

    this vector can be used to make modRNA expressing Cas9 and puro resistance gene

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

    Insert

    Cas9-2A-puro
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. hBACCS2-IRES-mCherry

    Plasmid

    #72892

    Purpose

    Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and mCherry

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    hBACCS2-IRES-mCherry
    

    Use

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. CIBN-CAAX

    Plasmid

    #79574

    Purpose

    expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting

    Depositor

    Olof Idevall-Hagren, Pietro De Camilli

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    CIBN (CIB1 Mustard Weed)
    

    Use

    Tags

    C-terminal CAAX-box

    Expression

    Mammalian

    Mutation

    K93A, R94A, K106A, K107A (please see depositor co…

    Promoter

    CMV

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAT217

    Plasmid

    #134885

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABEpuro-ARAF

    Plasmid

    #51076

    Purpose

    mammalian expression of wt ARAF

    Depositor

    Matthew Meyerson

    Article

    Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

    Insert

    ARAF (ARAF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CaMKIIa-hM3D(Gq)-mCherry (AAV1)

    Viral Prep

    #50476-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-hM3D(Gq)-mCherry (#50476). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM3D(Gq)-mCherry plasmid DNA. CaMKIIa-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bryan Roth

    Promoter

    CaMKII

    Tags

    None

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

20. p3E-Rac1-DN

    Plasmid

    #109582

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative Rac1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Rac1 (rac1a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    dominant negative

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET16b-Trx

    Plasmid

    #36944

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Thioredoxin 1 (TXN Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAV-U6+27-Squash

    Plasmid

    #177913

    Purpose

    Expresses Squash aptamer in mammalian cells using the U6+27 promoter.

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Squash aptamer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)

    Plasmid

    #110560

    Purpose

    miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insert

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    stRBS-lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhaSR-PrhaBAD

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA3-FLAG-HA-hYTHDC1

    Plasmid

    #85167

    Purpose

    Expression of FLAG-HA-tagged human YTHDC1

    Depositor

    Samie Jaffrey

    Article

    Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

    Insert

    YTHDC1 (YTHDC1 Human)
    

    Use

    Tags

    Flag and Ha

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

    Plasmid

    #132393

    Purpose

    Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    human TauP301L 2N4R-EGFP (MAPT Synthetic, Human)
    

    Use

    CRISPR and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Proline 301 to Leucine (P301L)

    Promoter

    TREtight

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCMV-SynB

    Plasmid

    #174861

    Purpose

    Expresses mouse syncytin B in mammalian cells

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    syncytin B (Synb Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV6M-Pak1

    Plasmid

    #12209

    Depositor

    Jonathan Chernoff

    Article

    Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

    Insert

    Pak1 (PAK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP-ATF6

    Plasmid

    #32955

    Purpose

    For visualization of GFP-ATF6 in mammalian cells.

    Depositor

    Ron Prywes

    Article

    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

    Insert

    ATF6 (ATF6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    M67L and M313I

    Promoter

    CMV

    Available since

    Nov. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMJ920

    Plasmid

    #42234

    Depositor

    Jennifer Doudna

    Article

    Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    GFP, HA epitope, and NLS (nuclear localization si…

    Expression

    Mammalian

    Mutation

    codon-optimized synthetic DNA sequence

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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30. pMLS7

    Plasmid

    #32056

    Purpose

    Vector optimized for constitutive gene expression in Burkholderia genus and related bacteria

    Depositor

    Miguel Valvano

    Article

    Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ps7 promoter, originated from Paraburkholderia xe…

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge