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We narrowed to 25,285 results for: crispr

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Showing: 41 - 60 of 25285 results

  2. pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

    Plasmid
    #42333
    Purpose
    This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.
    Depositor
    Feng Zhang
    Publication
    Cong et al Science. 2013 Jan 3.
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Available since
    March 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    42333-plasmid-map-sequence-id-59273

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  4. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid
    #90085
    Purpose
    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Publication
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    14 Articles
    90085-plasmid-map-sequence-id-182691

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  5. pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)

    Plasmid
    #79620
    Purpose
    Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells
    Depositor
    Akihiko Kondo
    Publication
    Nishida et al Science. 2016 Aug 4. pii: aaf8729.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9
    Tags
    PmCDA1 and UGI
    Expression
    Mammalian
    Mutation
    D10A for nickase Cas9
    Promoter
    pCMV
    Available since
    July 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    16 Articles
    79620-plasmid-map-sequence-id-154120

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  8. Human sgRNA library Brunello in lentiCRISPRv2 (Lentiviral Prep)

    Viral prep
    #73179-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiCRISPRv2 (#73179). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2 plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. This backbone contains SpCas9 and unique gRNAs, and can be used to make edits across 19,114 genes in the human genome. In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2.
    Depositor
    John Doench, David Root
    Publication
    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103
    Available since
    June 14, 2017
    Availability
    Academic Institutions and Nonprofits only

  10. pFGC-pcoCas9

    Plasmid
    #52256
    Purpose
    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA
    Depositor
    Jen Sheen
    Insert
    35SPPDK:pcoCas9:NOS cassette
    Use
    CRISPR; Plant expression
    Promoter
    constitutive 35SPPDK promoter
    Available since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    15 Articles
    52256-plasmid-map-sequence-id-116013

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  12. pX330A-nBRD4/PITCh

    Plasmid
    #91794
    Purpose
    PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4
    Depositor
    James Bradner, Behnam Nabet
    Publication
    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    chicken β-actin promoter
    Available since
    March 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    91794-plasmid-map-sequence-id-429087

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  14. MLM3613

    Plasmid
    #42251
    Purpose
    Expresses Cas9 nuclease (Streptococcus pyogenes) from CMV and T7 promoters
    Depositor
    Keith Joung
    Publication
    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Streptococcus pyogenes Cas9
    Use
    CRISPR; Zebrafish expression
    Promoter
    CMV
    Available since
    Jan. 31, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114 Articles
    42251-plasmid-map-sequence-id-59208

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  18. pMJ920

    Plasmid
    #42234
    Depositor
    Jennifer Doudna
    Publication
    Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Use
    CRISPR
    Tags
    GFP, HA epitope, and NLS (nuclear localization si…
    Expression
    Mammalian
    Mutation
    codon-optimized synthetic DNA sequence
    Promoter
    CMV
    Available since
    March 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    42 Articles
    42234-plasmid-map-sequence-id-57040

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  20. pDD162 (Peft-3::Cas9 + Empty sgRNA)

    Plasmid
    #47549
    Purpose
    Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.
    Depositor
    Bob Goldstein
    Publication
    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm
    Inserts
    Cas9
    Empty sgRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Worm
    Mutation
    Codon optimized and with synthetic introns for C.…
    Promoter
    U6 and eef-1A.1 (eft-3)
    Available since
    Sept. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    159 Articles
    47549-plasmid-map-sequence-id-228099

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Showing: 41 - 60 of 25285 results