161,511 results

1. pHR Lck-TagBFP

   Plasmid

   #171034

   Purpose

   TagBFP-tagged Lck kinase

   Depositor

   Jared Toettcher

   Article

   Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

   Insert

   Lck
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPCX-PECAMTS

   Plasmid

   #45850

   Depositor

   Martin Schwartz

   Article

   Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

   Insert

   PECAM1 (PECAM1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. FPR3-Tango

   Plasmid

   #66285

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   FPR3 (FPR3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-20b-M-Ntx-S1

   Plasmid

   #58716

   Purpose

   Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

   Depositor

   Fiorella Tonello

   Article

   Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

   Insert

   Notexin
   

   Use

   Tags

   Methionine

   Expression

   Bacterial

   Mutation

   Changed Asparagine 1 to Serine

   Promoter

   T7

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPIK16045_pX330_Flag-SuperFi-Cas9

   Plasmid

   #184370

   Purpose

   Mammalian expression of SuperFi-Cas9 nuclease

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   3X FLAG and NLS

   Expression

   Mammalian

   Mutation

   Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K…

   Promoter

   CBh

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Rat Alpha2 delta-1 HA pMT2

   Plasmid

   #58729

   Purpose

   expression of HA tagged rat Alpha2 delta-1 in mamalian cells

   Depositor

   Annette Dolphin

   Article

   Kadurin et al J Biol Chem. 2012 Sep 28;287(40):33554-6

   Insert

   alpha2 delta-1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Neuroligin-1-GFP/pCMV

   Plasmid

   #197340

   Purpose

   Plasmid expressing an antigen targeting Neuroligin-1

   Depositor

   Thomas Südhof

   Insert

   Neuroligin1 (Nlgn1 Mouse)
   

   Use

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETSUMO2_Bacteroidetes-bGSDM-TEV

   Plasmid

   #222101

   Purpose

   For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

   Insert

   Bacteroidetes-bGSDM-TEV ORF
   

   Use

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   TEV protease site replaces residues E242–G248

   Promoter

   T7

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-CMV-MLH1dn-IRES-puroR

   Plasmid

   #207354

   Purpose

   Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   MutL homolog 1 (MLH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Δ754-756 dominant negative mutant

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO-Tet-On shYAP1-9

    Plasmid

    #193668

    Purpose

    Tet inducible knockdown of YAP1

    Depositor

    William Hahn

    Article

    Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_ATF4_WT

    Plasmid

    #82190

    Purpose

    Gateway Donor vector containing ATF4 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Rich-pT7

    Plasmid

    #108445

    Purpose

    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pUC57. Xenopus globin 3UTR and 5UTR, pT7, and polyA added.

    Depositor

    Richard Bélanger

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    pT7

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSGEMKv10.1

    Plasmid

    #85704

    Purpose

    Xenopus laevis oocyte expression of Kv10.1

    Depositor

    Luis Pardo

    Article

    Jenke et al EMBO J. 2003 Feb 3;22(3):395-403.

    Insert

    KCNH1 (KCNH1 Human)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. DDX5

    Plasmid

    #155523

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. NRIP1_pCSdest

    Plasmid

    #53805

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    NRIP1 (NRIP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pDONR223_GOPC_WT

    Plasmid

    #82924

    Purpose

    Gateway Donor vector containing GOPC, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, Matthew Meyerson

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    GOPC (GOPC Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. rAAV-syn::FLEX-rev::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

    Plasmid

    #32475

    Purpose

    Cre-dependent expression of PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

    Insert

    PSAMQ79G,Q139G-5HT3HC
    

    Use

    AAV and Cre/Lox; Adeno-associated virus, flex swi…

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    Synapsin

    Available since

    Dec. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221_SLC25A3

    Plasmid

    #132040

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A3 (SLC25A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pIVT

    Plasmid

    #122139

    Purpose

    For making cRNA appropriate for microinjection

    Depositor

    Carmen Williams

    Article

    Igarashi et al Dev Biol. 2007 Dec 1;312(1):321-30. Epub

    Type

    Empty backbone

    Use

    For in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET24a-LaM4-EGFP

    Plasmid

    #182641

    Purpose

    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    anti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, EGFP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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