167,318 results

1. pAAV-CAG-SV40NLSf-GFP-3xmiR122-WPRE-HGHpA

   Plasmid

   #183775

   Purpose

   AAV genome with a CAG driven eGFP reporter with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

   Depositor

   Ben Deverman

   Article

   Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

   Insert

   NLS-GFP
   

   Use

   AAV

   Mutation

   NA

   Promoter

   CAG

   Available Since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. P362-FBP_12

   Plasmid

   #139703

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   various

   Available Since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-C1 actin-3XNLS P2A mCherry

   Plasmid

   #58475

   Purpose

   Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

   Inserts

   beta actin (ACTB Human)
   3XNLS
   P2A cleavage site
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-BAZ1A

   Plasmid

   #65371

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BAZ1A (BAZ1A Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   796I compared to all reference sequences

   Promoter

   CMV

   Available Since

   June 15, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pGL4.10 Dub3

   Plasmid

   #72684

   Purpose

   luciferase reporter with Dub3 promoter

   Depositor

   Domenico Maiorano

   Article

   van der Laan et al PLoS One. 2014 Apr 2;9(4):e93663. doi: 1

   Insert

   Dub3 promoter (Usp17le Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Dub3

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-Wnt7B

   Plasmid

   #35915

   Depositor

   Marian Waterman

   Article

   Najdi et al Differentiation. 2012 Sep;84(2):203-13.

   Insert

   Wnt7B (Wnt7b Human)
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. L13-Arl13bGFP

   Plasmid

   #40879

   Purpose

   3rd generation transfer vector

   Depositor

   Tamara Caspary

   Article

   Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

   Insert

   Arl13b (Arl13b Mouse)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDV05.0_pEF1a-iRFP720

   Plasmid

   #200407

   Purpose

   Expresses iRFP720 constitutively in mammalian cells

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   iRFP720
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWXC077

   Plasmid

   #222960

   Purpose

   C. elegans codon-optimized mScarlet3 with 3 introns cloned into pPD95.75 backbone

   Depositor

   Oliver Hobert

   Article

   Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

   Insert

   mScarlet3
   

   Expression

   Worm

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-EF1a-BsdCas9-W

    Plasmid

    #67978

    Purpose

    Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    EF1a-Cas9 cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Cas9 fused with Flag-tag and a NLS at the N termi…

    Promoter

    EF1a

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCRISPR-E

    Plasmid

    #78852

    Purpose

    Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

    Depositor

    Phillip Abbosh

    Article

    Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Cas9-P2A-Puro

    Expression

    Mammalian

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. CL20_mEGFP_NPM1_ALK

    Plasmid

    #205868

    Purpose

    Express mEGFP-tagged fusion protein, NPM1_ALK from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    NPM1_ALK (NPM1, ALK Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138133

    Purpose

    Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    EGFP-anti-rabbit-IgG(Fc)-nanobody
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBad-sfGFP 150TAG

    Plasmid

    #85483

    Purpose

    Arabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

    Insert

    superfolder GFP 150TAG
    

    Expression

    Bacterial

    Mutation

    150TAG (amino acid 150 mutated to stop codon)

    Promoter

    Arabinose

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ202

    Plasmid

    #86198

    Purpose

    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromiycin resistance marker

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Type

    Empty backbone

    Use

    Gateway compatible attr1-attr2 destination vector

    Expression

    Plant

    Promoter

    Arabidopsis ubiquitin 10 (AtUbi10)

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUB6-HuMGF

    Plasmid

    #190782

    Purpose

    Expresses membrane-bound form of human SCF in mammalian cells

    Depositor

    Jakob Reiser

    Article

    Zhang et al Retrovirology. 2010 Jan 25;7:3. doi: 10.

    Insert

    human stem cell factor (KITLG Human)
    

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB6364

    Plasmid

    #106151

    Purpose

    EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Insert

    Cas9
    

    Expression

    Yeast

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. SARS-CoV-2 S HexaPro

    Plasmid

    #154754

    Purpose

    Mammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant

    Depositor

    Jason McLellan

    Article

    Hsieh et al Science. 2020 Sep 18;369(6510):1501-1505

    Insert

    Spike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Available Since

    July 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. wt dynamin 2 pEGFP

    Plasmid

    #34686

    Depositor

    Sandra Schmid

    Insert

    dynamin 2 (Dnm2 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D406G, V664A, H692R and Q758R

    Promoter

    CMV

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. Str-KDEL_TNF-SBP-EGFP

    Plasmid

    #65278

    Purpose

    synchronize trafficking of TNF from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. p16sheLL

    Plasmid

    #37320

    Depositor

    John Schiller

    Article

    Buck et al PLoS Pathog. 2006 Jul;2(7):e69.

    Insert

    HPV16L1+L2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-CMV-FLEX-SaCas9-U6-sgRosa26

    Plasmid

    #159914

    Purpose

    Mutagenesis of Rosa26 with SauCas9

    Depositor

    Larry Zweifel

    Insert

    Rosa26 gRNA (Gt(ROSA)26Sor Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pscALPS- SIV-MAC251 vpx

    Plasmid

    #115810

    Purpose

    Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVMAC251 Vpx
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Promoter

    SFFV

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA3.1-HA-UBA6

    Plasmid

    #136995

    Purpose

    Expresses human UBA6 in mammalian cells

    Depositor

    Marcus Groettrup

    Article

    Pelzer et al J Biol Chem. 2007 Aug 10;282(32):23010-4

    Insert

    UBA6 (UBA6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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25. pDG458

    Plasmid

    #100900

    Purpose

    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCR4-24XPP7SL

    Plasmid

    #31864

    Depositor

    Robert Singer

    Article

    Larson et al Science. 2011 Apr 22;332(6028):475-8.

    Insert

    24xPP7 Stem Loop Cassette
    

    Available Since

    Aug. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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27. pXD024 - pLKO-EF1a-GFP-P2A-FLuc-WPRE

    Plasmid

    #192204

    Purpose

    Lenti-GL(GFP-Luciferase)

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Lenti-GL(GFP-Luciferase)
    

    Use

    Lentiviral; Mammalian expression

    Mutation

    NA

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pWN_U6-B2M-miniGag-Cas9

    Plasmid

    #228958

    Purpose

    miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag-Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 764 pME nls-mApple

    Plasmid

    #195962

    Purpose

    Gateway pME fluorophore

    Depositor

    Kristen Kwan, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mApple nuclear
    

    Use

    Other

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ISceI-GR-RFP

    Plasmid

    #17654

    Depositor

    Tom Misteli

    Article

    Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

    Insert

    I-SceI-GRLBD (SCEI, Nr3c1 Rat, Budding Yeast)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    C655G mutation in the glucocorticoid receptor (se…

    Available Since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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