We narrowed to 60,538 results for: Gene
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Plasmid#65777PurposeHuman expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNADepositorInsertSpCas9 gRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPSU2
Plasmid#89566PurposeHigh copy number plasmid 2 to prepare Penn State DNA molecular weight laddersDepositorInserts750 bp EcoRV fragment
3000 bp EcoRV fragment
4000 bp EcoRV fragment
50 bp PstI fragment
100 bp PstI fragment
200 bp PstI fragment
300 bp PstI fragment
400 bp PstI fragment
500 bp PstI fragment
600 bp PstI fragment
1500 bp Psti fragment
4100 bp PstI fragment
ExpressionBacterialAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPSU1
Plasmid#89439PurposeHigh copy number plasmid 1 to prepare Penn State DNA molecular weight laddersDepositorInsertsExpressionBacterialAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
BL21ΔABCF
Bacterial Strain#102270PurposeompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteinsDepositorBacterial ResistanceTetracyclineAvailable SinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-HA-hUVRAG
Plasmid#24290DepositorAvailable SinceJuly 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAc-sgRNA-Cas9
Plasmid#49330PurposeExpresses sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertsCas9
dU6-sgRNA
UseCRISPRTags3xFLAG and NLSExpressionInsectMutationHuman codon optimisedPromoterActin-5c and Drosophila U6Available SinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
BJ5183 cells
Bacterial Strain#16398DepositorBacterial ResistanceStreptomycinSpeciesEscherichia coliAvailable SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8m-WPRE (AAV1)
Viral Prep#162375-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP8m-WPRE (#162375). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8m-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP
Plasmid#37825PurposeAAV-mediated expression of GFP under the CAG promoterDepositorHas ServiceAAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV11, AAV11 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV6, AAV6 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1InsertGFP
UseAAV; Adeno-associated virusExpressionMammalianMutationN/APromoterCAGAvailable SinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
T7opt in pCAGGS
Plasmid#65974PurposeHuman codon-optimized T7 polymeraseDepositorInsertT7-opt
ExpressionMammalianAvailable SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-Cas9-His
Plasmid#47327PurposeFor in vitro expression and purification of Cas9 proteinDepositorInsertCas9
UseCRISPRTagsHisExpressionBacterialAvailable SinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-T2A-GFP
Plasmid#53190Purpose3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFPDepositorInserthumanized Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianPromoterhUbCAvailable SinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK5-EGFP-Tau
Plasmid#46904Purposeexpresses EGFP tagged Tau in mammalian cellsDepositorInsertMAPT (MAPT Human)
TagsEGFPExpressionMammalianMutationThis WT htau construct contains human four-repeat…PromoterCMVAvailable SinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC-EHER
Plasmid#53262PurposeContains a 7 gene carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin diglucoside in Escherichia coliDepositorInsertcrtE, idi, crtX, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-loxPSTOPloxP-ZsGreen
Plasmid#51269PurposeCre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by loxP sites followed by ZsGreenDepositorInsertloxP-STOP-loxP-ZsGreen
ExpressionMammalianPromoterCAGAvailable SinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-dLight1.2 (AAV5)
Viral Prep#111068-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-hSyn-dLight1.2 (#111068). In addition to the viral particles, you will also receive purified pAAV-hSyn-dLight1.2 plasmid DNA. Synapsin-driven expression of dLight1.2 dopamine sensor. This sensor has a slightly increased dynamic range and is better for 2P imaging in vivo. These AAV preparations are suitable purity for injection into animals.DepositorAvailable SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2 blast
Plasmid#98293PurposeVariant of lentiCRISPRv2 that confers blasticidin S resistanceDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralPromoterEF-1aAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAdTrack-CMV
Plasmid#16405PurposeShuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseAdenoviralExpressionMammalianAvailable SinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Flpe
Plasmid#13787PurposeMammalian expression of enhanced form of Flp recombinase with C-terminal Myc tagDepositorInsertFlpe
TagsMycExpressionMammalianPromoterCAGAvailable SinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-mCherry-P2A-Cre-WPRE (AAV1)
Viral Prep#107312-AAV1PurposeReady-to-use AAV1 particles produced from AAV-hSyn-mCherry-P2A-Cre-WPRE (#107312). In addition to the viral particles, you will also receive purified AAV-hSyn-mCherry-P2A-Cre-WPRE plasmid DNA. hSyn-driven expression of mCherry and Cre (physically separate). These AAV preparations are suitable purity for injection into animals.DepositorTagsmCherry (physically separate, not a fusion protein)Available SinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pBpF
Plasmid#31190PurposetRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.DepositorInsertM.j. p-benzoylphenylalanine RS (2 copies +tRNA)
ExpressionBacterialMutationY32G, E107P, D158T, I159SAvailable SinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-1
Plasmid#16400PurposeAdenoviral vector for use in AdEasy System.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseAdenoviralExpressionMammalianAvailable SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
p414-TEF1p-Cas9-CYC1t
Plasmid#43802PurposeA human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoterDepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRExpressionYeastPromoterTEF1 promoterAvailable SinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP
Plasmid#50716Purpose5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.DepositorInsertsplit EGFP
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
sortase A pentamutant (eSrtA) in pET29
Plasmid#75144Purposeevolved sortase A (eSrtA) pentamutant with improved kinetics and activityDepositorInsertsortase A pentamutant
Tags6x HisExpressionBacterialMutationP94R, D160N, D165A, K190E, K196T. Deleted amino a…PromoterT7Available SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE EF1α dCas9-KRAB
Plasmid#50919PurposeConstitutive dCas9-KRAB 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and KRABExpressionMammalianMutationD10A, H840APromoterEF1alphaAvailable SinceMarch 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
FUdeltaGW-rtTA
Plasmid#19780Purpose3rd generation lentiviral vectorDepositorInsertreverse tetracycline transactivator
UseLentiviralExpressionMammalianAvailable SinceFeb. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLIX_403
Plasmid#41395PurposeInducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTADepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Gateway destination vector, doxycycli…TagsV5ExpressionMammalianPromoterTRE promoter, Tet ONAvailable SinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDH-UTY-F
Plasmid#40617DepositorAvailable SinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
Cre-IRES-PuroR
Plasmid#30205PurposeLentiviral coexpression of Cre and puromycin from the human EF1a promoterDepositorInsertCre recombinase (cre Enterobacteria phage P1)
UseCre/Lox and LentiviralExpressionMammalianPromoterEF1alphaAvailable SinceApril 12, 2012AvailabilityAcademic Institutions and Nonprofits only