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We narrowed to 18,824 results for: nls

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Showing: 601 - 620 of 18824 results

  3. pET-PE2-His (IK1822)

    Plasmid
    #170103
    Purpose
    T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS
    Depositor
    Keith Joung
    Publication
    Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103
    Insert
    bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
    Use
    CRISPR
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Bacteria codon-optimized nSpCas9 (H840A) & M-…
    Promoter
    T7
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    170103-plasmid-map-sequence-id-332603

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  4. nCre-ST-NLS-P2A-NLS-pSC-cCre

    Plasmid
    #207638
    Purpose
    A plasmid encoding N-terminal Cre fused to SpyTag (ST) and photocaged SpyCatcher (pSC) fused to C-terminal Cre with a nuclear localization signal (NLS) sequence
    Depositor
    Cole DeForest
    Publication
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    nCre-SpyTag-NLS-P2A-NLS-photocaged SpyCatcher-cCre
    Expression
    Mammalian
    Mutation
    Amber stop codon at SpyCatcher’s critical lysine
    Promoter
    CMV
    Available since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207638-plasmid-map-sequence-id-409880

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  8. pCAGEN-nls-mCherry-stop-IRES-myr-EGFP (JDW 1326)

    Plasmid
    #229812
    Purpose
    A CAGGS driven expression vector containing an nls-mCherry followed by an IRES and then a myristoylated EGFP for labeling the cell membrane.
    Depositor
    Joshua Wythe
    Publication
    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nls-mCherry
    Tags
    SV40 NLS
    Expression
    Mammalian
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229812-plasmid-map-sequence-id-460327

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  12. pC015 - dLwCas13a-NF

    Plasmid
    #91905
    Purpose
    Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging
    Depositor
    Feng Zhang
    Publication
    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLwCas13a
    Use
    CRISPR
    Tags
    NLS and eGFP, ZF CCR5TC, KRAB-A, NLS
    Expression
    Mammalian
    Mutation
    R474A and R1046A
    Promoter
    CAG
    Available since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91905-plasmid-map-sequence-id-198453

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  17. pACT2-CAS9c

    Plasmid
    #51055
    Purpose
    Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.
    Depositor
    Yunde Zhao
    Publication
    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1
    Insert
    Cas9c
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Bacterial and Yeast
    Promoter
    yeast ADH1 promoter
    Available since
    Feb. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51055-plasmid-map-sequence-id-77327

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Showing: 601 - 620 of 18824 results