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pHis-hMTMR1

Plasmid

#100723

Purpose

Expression of human MTMR1 PH-PTP domain

Depositor

Byung Il Lee

Article

Bong et al PLoS One. 2016 Mar 28;11(3):e0152611. do

Insert

MTMR1 (MTMR1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains amino acids 95-607

Promoter

T7

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pPL5618_pUG_MtxR

Plasmid

#60929

Purpose

PCR template vector for generating methotrexate resistance cassette.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Dihydrofolate reductase (DHFR Human)

Use

Loxp flanked deletion cassette template

Mutation

L22F F31S

Promoter

TEF1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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SUMO_MT3

Plasmid

#200303

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

Depositor

Artur Krężel

Article

Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

Insert

metallothionein 3 (MT3 Human)

Tags

Smt3

Expression

Bacterial

Promoter

T7

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-C7orf59

Plasmid

#42336

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

C7orf59 (LAMTOR4 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pEG302 22aa SunTag VP64 gRNA4 (FWA)

Plasmid

#120249

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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PERK 4: PERKKD-pGEX4T-1

Plasmid

#21817

Depositor

David Ron

Article

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (537-1114) (Eif2ak3 Mouse)

Tags

GST and Myc (9E10)

Expression

Bacterial

Mutation

PERK Kinase Domain. Amino acids 537-1114.

Available Since

March 29, 2010

Availability

Academic Institutions and Nonprofits only

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pMIEG3-BCL6

Plasmid

#40339

Depositor

Alexander Dent

Article

Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Insert

BCL6 (BCL6 Human)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Mutation

optimized translation initiation codon

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
pGL3_5UTR_Myc_FLuc

Plasmid

#229502

Purpose

Myc 5' untranslated region firefly luciferase reporter.

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

Myc 5'UTR

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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Luc-SIRT1 mutant 3'UTR

Plasmid

#20380

Depositor

Charles Lowenstein

Article

Yamakuchi et al Proc Natl Acad Sci U S A. 2008 Sep 9. 10

Insert

SIRT1 (SIRT1 Human)

Use

Luciferase

Mutation

mutant 3'UTR of SIRT1 (ACACCCACCAAGGACCATTAG…

Available Since

May 1, 2009

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag VP64 g4+g17

Plasmid

#120252

Purpose

CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 17, 2019

Availability

Academic Institutions and Nonprofits only

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LRP6N-IgG-pCS2

Plasmid

#27279

Depositor

Xi He

Article

Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.

Insert

low density lipoprotein receptor-related protein 6 (LRP6 Human)

Use

Xenopus injection

Tags

IgG Fc

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)

Plasmid

#115487

Purpose

CRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAs

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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STAGR_SAMScaffold_hU6

Plasmid

#102843

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_hU6promoter

Use

As pcr template

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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PXPR007 sgGFP-1

Plasmid

#74960

Purpose

Cas9 + sgGFP-1 with blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

GFP

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEG302 22aa SunTag NtDRMcd gRNA4 (FWA)

Plasmid

#115486

Purpose

CRISPR-Cas9 SunTag system to target NtDRMcd to the FWA locus with a single guide RNA

Depositor

Steven Jacobsen

Article

Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

Insert

g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pTn7·X-nia

Plasmid

#122592

Purpose

Plasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion site

Depositor

Pablo Ivan Nikel

Article

Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

xylS/Pm->Nia

Use

Synthetic Biology; Tn7 genomic integration

Expression

Bacterial

Promoter

Pm

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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LZF1826 pAAV_hSyn-DiO-SomArchon-EGFP-P2A-stGtACR2

Plasmid

#135412

Purpose

simultaneous optical recording and manipulation of electrical activity in neurons

Depositor

Adam Cohen

Article

Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

Insert

SomArchon-EGFP-P2A-stGtACR2

Use

AAV

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA HisB TO rFez1

Plasmid

#17385

Depositor

Stephen Goff

Article

Naghavi et al Genes Dev. 2005 May 1. 19(9):1105-15.

Insert

FEZ1 (Fez1 Rat)

Tags

his and myc

Expression

Mammalian

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
pHAGE-UBC-TagRFP-T-ps

Plasmid

#178656

Purpose

Expression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cells

Depositor

Jeffrey Gerst

Article

Haimovich et al Proc Natl Acad Sci U S A. 2017 Nov 14;11

Insert

TagRFP-T with palmitoylation signal at N-terminus

Use

Lentiviral

Tags

Palmitoylation signal

Promoter

Ubc

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_tKiMBI-T2A-caMEK

Plasmid

#199578

Purpose

Expresses tKiMBI and caMEK in an AAV vector

Depositor

Michael Lin

Article

Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

Inserts

ERK tdTomato-Kinase-Modulated Bioluminescent Indicator
constitutively active MEK

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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