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pET-Neq2X7

Plasmid

#182366

Purpose

Expresses Neq2X7 DNA polymerase

Depositor

Morten Norholm

Article

Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

Insert

Neq2X7 DNA polymerase

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pKD238-1a

Plasmid

#90958

Purpose

Expresses S. baltica TtrS constitutively

Depositor

Jeffrey Tabor

Article

Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

Insert

Tetrathionate sensor kinase

Expression

Bacterial

Promoter

Bba_J23109

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pWUR702

Plasmid

#53079

Purpose

Inducible TtAgo expression

Depositor

John van der Oost

Article

Swarts et al Nature. 2014 Mar 13;507(7491):258-61. do

Insert

TtAgo

Tags

Strep-II tag

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_MeltCasp1-37-GFP

Plasmid

#234069

Purpose

Encodes the caspase1 without CARD domain (with R391K mutation) controlled by a Melt variant with a switching temperature of 37°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

MeltCasp1-37-GFP

Use

Lentiviral

Expression

Mammalian

Mutation

Melt(C292A,Q355N); hcaspase1(R391K)

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-URA

Plasmid

#160004

Purpose

Low-copy expression vector for K. marxianus, uracil selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

n/a

Expression

Yeast

Promoter

n/a

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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XBP-1 2: pFLAG.XBP1p.CMV2

Plasmid

#21833

Depositor

David Ron

Article

Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

Insert

XBP-1 processed/spliced (Xbp1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pLX208-cytoFLARE1.0-TF

Plasmid

#234513

Purpose

Transcriptional reporter for detecting calcium increase in HEK293T (transcription factor)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

ERT2-MK2-hLOV-TEVcs-NLS-GAL4-FLAG

Use

Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGNW6

Plasmid

#122093

Purpose

suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe-tetoff-mVenus-PICdc42

Plasmid

#91876

Purpose

PI(WT): Photo inhibitable (blue light sensitive)

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Vav2 (VAV2 Human)

Tags

mVenus

Expression

Bacterial and Mammalian

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-MTS-jAspSnFR3-mRuby3

Plasmid

#203492

Purpose

Mitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

MTS-jAspSnFR3-mRuby3

Use

Lentiviral

Tags

His-tag, Mitochondrial localization signal, and m…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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BLA_CAT_K136_GPG_LOV2

Plasmid

#231575

Purpose

This plasmid mediates chloramphenicol resistance in the dark, resulting in Cm mediated cell death upon illumination with blue light

Depositor

Dominik Niopek

Article

Wolf et al bioRxiv 2024.12.04.626757

Insert

CAT_ carrying an AsLOV2 insertion behind K136

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pFH2.156_LV-Zeo

Plasmid

#205554

Purpose

Express zeocin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Zeo

Use

Lentiviral

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK-rABE-Flag

Plasmid

#191383

Purpose

Doxycycline inducible expression of RNA A-to-I editor rABE

Depositor

Stephen Floor

Article

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

rABE-Flag

Use

Lentiviral and Synthetic Biology

Tags

Flag

Expression

Mammalian

Promoter

tet-inducible promoter

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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VV073: 1xCox8 - GCaMP5G in fubi

Plasmid

#58509

Purpose

Expresses the calcium sensor GCaMP5G in the mitochondria

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - GCaMP5G

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pBT114

Plasmid

#138523

Purpose

phage cloning

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

phage genes

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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Synapsin-pm-iATPSnFR1.0

Plasmid

#102555

Purpose

expresses membrane displayed ATP sensor in neurons

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Use

AAV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pS448∙CsR

Plasmid

#122096

Purpose

CRISPR/Cas9 counterselection in Gram-negative bacteria

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Inserts

Cas9
sgRNA

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pem7 and Pm

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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paavCAG-JxON-pre-mGRASP-mCerulean

Plasmid

#51903

Purpose

JxOn-pre

Depositor

Jinhyun Kim

Article

Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

Insert

presynaptic mGRASP

Use

AAV and Cre/Lox

Promoter

CAG

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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mEGFP-2xbiRhoBAST

Plasmid

#196349

Purpose

Expression of mEGFP mRNA with 2xbiRhoBAST in 3'UTR

Depositor

Murat Sunbul

Article

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Insert

mEGFP-2xbiRhoBAST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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HIV-1 integrase F185K/C280S in pET-15b

Plasmid

#61669

Purpose

HIV-1 integrase F185K/C280S in pET-15b

Depositor

Robert Craigie

Article

Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

Insert

HIV-1 integrase F185K/C280S

Use

Synthetic Biology

Tags

His

Promoter

T7 promotor

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
mEGFP-4xbiRhoBAST

Plasmid

#196350

Purpose

Expression of mEGFP mRNA with 4xbiRhoBAST in 3'UTR

Depositor

Murat Sunbul

Article

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Insert

mEGFP-4xbiRhoBAST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pSMVP-Cas9FL-P2A-turboGFP

Plasmid

#80941

Purpose

Plasmid that expresses Cas9FL in mammalian cells; co-expresses turboGFP.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9FL

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_Tbet_T2A_GFP_PGK_mCherry

Plasmid

#85431

Purpose

Lentiviral vector for Gal4 inducible Tbet T2A GFP and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human Tbet

Use

Lentiviral

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-CreiLOV

Plasmid

#192817

Purpose

Expresses CreiLOV in bacteria

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

CreiLOV

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDM1081

Plasmid

#108981

Purpose

Knockout cassette Hygromycin

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

Knockout cassette Hygromycin

Use

Cre/Lox; Dictyostelium discoideum knockout vector

Promoter

coaA

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-SpyTagMBP

Plasmid

#35050

Depositor

Mark Howarth

Article

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyTagMBP

Tags

His6

Expression

Bacterial

Mutation

Simple peptide tag added to the N-terminus of MBP…

Promoter

T7 promoter

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pGEMT-PTE2A

Plasmid

#111773

Purpose

Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Type

Empty backbone

Use

Cloning intermediate

Promoter

T7

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBT29

Plasmid

#138521

Purpose

phage cloning

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

phage genes

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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NM9-SpCas9-NLS3

Plasmid

#128177

Purpose

Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

SpCas9-NLS3

Use

CRISPR

Expression

Yeast

Promoter

pPGK1

Available Since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

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pDNR-mCherry-T2A-3xPuroR-Frame 1

Plasmid

#206992

Purpose

Donor plasmid for Frame 1 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

Depositor

Alejandro Chavez

Article

Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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