We narrowed to 2,107 results for: Endog

Showing: 641 - 660 of 2107 results

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qTAG-AAVS1-Ef1a-Puro

Plasmid

#227266

Purpose

Empty AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-mTrim21

Plasmid

#105516

Purpose

Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42(Q61L)

Plasmid

#12600

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

EGFP-Cdc42 (Q61L) (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q61L

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pFUGW mCherry-KASH

Plasmid

#131505

Purpose

mCherry_KASH epression under hUBC promotor; serves as backbone for ORANGE lenti knock-in

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

mCherry_KASH

Use

Lentiviral

Expression

Mammalian

Promoter

hUBC

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSFFV-SpyTag-sfCherry2(11)-TagBFP

Plasmid

#117485

Purpose

Expresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

SpyTag002-GG-sfCherry2(11)

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Promoter

SFFV

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pT2/LTR7-GFP

Plasmid

#62541

Purpose

LTR7-driving reporter

Depositor

Zsuzsanna Izsvak

Article

Wang et al Nature. 2014 Dec 18;516(7531):405-9. doi

Insert

LTR7-GFP

Use

Sleeping beauty transposon

Expression

Mammalian

Promoter

LTR7

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42(T17N)

Plasmid

#12601

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

EGFP-Cdc42 (T17N) (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T17N

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pLHCX-gpASNase1

Plasmid

#121526

Purpose

Expresses gpASNase1 in mammalian cells.

Depositor

Lucas Sullivan, Matthew Vander Heiden

Article

Sullivan et al Nat Cell Biol. 2018 Jul;20(7):782-788. d

Insert

gpASNase1

Use

Retroviral

Tags

FLAG

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pIZ-Flag6His-BmAgo3

Plasmid

#50559

Purpose

Expresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cells

Depositor

Yukihide Tomari

Article

Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

Insert

BmAgo3

Tags

6xHIS and Flag

Expression

Insect

Promoter

OpIE2

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

Plasmid

#117484

Purpose

Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

SpyCatcher002-6aa-sfCherry2(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher

Plasmid

#117656

Purpose

Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Inserts

sfCherry2(1-10)
sfCherry2(11)-SpyCatcher002

Expression

Bacterial

Promoter

T7

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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CaMKIIP-mEGFP-P2A-paAIP2 (R5AR6A)/pAAV

Plasmid

#91719

Purpose

AAV-mediated expression of mEGFP and paAIP2 (R5AR6A), the functional mutant of paAIP2

Depositor

Ryohei Yasuda

Article

Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

Insert

mEGFP-P2A-paAIP2(R5AR6A)

Use

AAV

Promoter

CaMKII 1.3

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-FLIM-AKAR

Plasmid

#63058

Purpose

Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKAR

Use

AAV

Expression

Mammalian

Promoter

EF-1α

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7

Plasmid

#172067

Purpose

Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP11x7

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSyn-RFP

Plasmid

#22907

Depositor

Edward Callaway

Article

Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

Insert

human Synapsin I promoter driving DsRedExpress (SYN1 Human)

Use

AAV; Adeno-associated virus

Expression

Mammalian

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pSMPP-mCherry-mTrim21

Plasmid

#104971

Purpose

Lentiviral Transfer Plasmid with mCherry-tagged mouse Trim21

Depositor

Leo James

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Tripartite Motif containing 21 (Trim21 Mouse)

Use

Lentiviral

Tags

mCherry

Promoter

SFFV

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-PGK-Puro

Plasmid

#227269

Purpose

Empty AAVS1 targeting donor for the insertion of Puro and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Punc-4c.TIR1

Plasmid

#194053

Purpose

TIR1 expression vector under the unc-4c promoter expressed in the DA motor neurons in C. elegans

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

TIR1 (TIR1 Mustard Weed)

Expression

Worm

Mutation

codon-optimized for expression in C. elegans; Two…

Promoter

unc-4c

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-mCAMK-RFP

Plasmid

#22908

Depositor

Edward Callaway

Article

Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

Insert

mouse alpha-calcium/calmodulin-dependent protein kinase II promoter (Camk2a Mouse)

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-AAVS1_sgRNA

Plasmid

#183890

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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