161,377 results

1. pCMV5-ActRIIB

   Plasmid

   #13501

   Depositor

   Jeff Wrana

   Article

   Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

   Insert

   Activin receptor IIB (Acvr2b Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pEGFP-C1-TFIIB

   Plasmid

   #26675

   Depositor

   Sui Huang

   Article

   Chen et al Mol Biol Cell. 2002 Jan . 13(1):276-84.

   Insert

   TFIIB (GTF2B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

3. Snap-Omp25

   Plasmid

   #69599

   Purpose

   Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

   Depositor

   David Sabatini

   Article

   Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

   Insert

   Snap-Omp25
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-CMV-mC-G-LC3B-P

   Plasmid

   #124974

   Purpose

   evaluate autophagy

   Depositor

   Kazuhiro Oka

   Article

   Sutton et al Autophagy. 2018;14(4):637-653. doi: 10.1

   Insert

   mCherry-GFP-LC3B
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pINDUCER20_mGFP

   Plasmid

   #190027

   Purpose

   Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal mEGFP

   Depositor

   Andrew Tutt

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Fab_8D3_2_H-H6

   Plasmid

   #176075

   Purpose

   Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   His6 tagged heavy chain of Fab_8D3_2
   

   Use

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Fab_8D3_2_L

   Plasmid

   #176076

   Purpose

   Express light chain of Fab_8D3_2 in mammalian cells

   Depositor

   Tom Rapoport

   Article

   Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   Light chain of Fab_8D3_2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-EF1a-BbTagBY (AAV9)

   Viral Prep

   #45185-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from AAV-EF1a-BbTagBY (#45185). In addition to the viral particles, you will also receive purified AAV-EF1a-BbTagBY plasmid DNA. Brainbow construct encoding farnesylated TagBFP and EYFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Dawen Cai, Joshua Sanes

   Article

   Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

   Promoter

   EF1a

   Tags

   TagBFP, EYFP, or none.

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

9. eGFP-KIF5B

   Plasmid

   #172203

   Purpose

   Expresses KIF5B tagged with eGFP in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

   Insert

   KIF5B (Kif5b Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF_PE2

    Plasmid

    #199267

    Purpose

    Mammalian expression of SpCas9 prime editor 2 under EF1α promoter

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    PE2-P2A-mp53DD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3/Myc-DNMT3B1

    Plasmid

    #35522

    Purpose

    Mammalian expression of human DNMT3B1 with myc tag

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3B1 (DNMT3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

    Plasmid

    #175526

    Purpose

    Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lifeact-HaloTag11 and Tomm20-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAW240

    Plasmid

    #170791

    Purpose

    P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution

    Depositor

    Chang Liu

    Article

    Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

    Insert

    p10B2-AT110
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    10B2 (P1 specific promoter)

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSDM052

    Plasmid

    #194341

    Purpose

    pUC mini-Tn7 vector expressing LseCas13a

    Depositor

    Joseph Bondy-Denomy

    Article

    Mendoza et al Nature. 2020 Jan;577(7789):244-248. doi:

    Insert

    Cas13a(Lse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid

    #183411

    Purpose

    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    Tet-on 3G
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1_sgRNA

    Plasmid

    #100554

    Purpose

    Expresses AAVS1 sgRNA. Target sequence: GGGGCCACTAGGGACAGGAT

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

    Insert

    AAVS1 sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. His6TEV-UbG76C

    Plasmid

    #75299

    Purpose

    Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    human Ubiquitin mutant G76 to C
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Glycine 76 to Cystine

    Promoter

    T7

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. GUT BuGZ FL

    Plasmid

    #84024

    Purpose

    Expression of GFP tagged BuGZ in human cells

    Depositor

    Patrick Paddison

    Article

    Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

    Insert

    BuGZ (ZNF207 Human)
    

    Use

    Lentiviral

    Tags

    TurboGFP

    Expression

    Mammalian

    Mutation

    Q394R

    Promoter

    UCOE / SFFV

    Available since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDrVSP-IRES2-EGFP

    Plasmid

    #80333

    Purpose

    Expresses Danio rerio VSP with separated EGFP in mammalian cell

    Depositor

    Yasushi Okamura

    Article

    Hossain et al J Biol Chem. 2008 Jun 27;283(26):18248-5

    Insert

    Daino rerio voltage-sensing phosphatase (tpte Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX459-HypaCas9-mR2-AAVS1_sgRNA

    Plasmid

    #183890

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Flag-HA-COPS5

    Plasmid

    #22541

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    COPS5 (COPS5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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22. Human LFA-1 alpha

    Plasmid

    #8630

    Depositor

    Timothy Springer

    Article

    Larson et al J Cell Biol 1989 Feb;108(2):703-12.

    Insert

    LFA1 alpha (ITGAL Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

23. DRD2-DuET

    Plasmid

    #213228

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    DRD2 (DRD2 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. pMSCV-IRES-GFP-human-Caspase-4

    Plasmid

    #183356

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-4 (CASP4 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pUC19/PLIN2-GFP

    Plasmid

    #202194

    Purpose

    knock-in donor vector of GFP to the C-terminus of endogenous human PLIN2

    Depositor

    James Olzmann

    Article

    Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

    Insert

    PLIN2 (PLIN2 Human)
    

    Use

    Cloning vector

    Tags

    GFP knock-in

    Expression

    Mutation

    Promoter

    lac

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pDAC435

    Plasmid

    #195238

    Purpose

    Mammalian expression of Csm complex from separate promoters; RFP backbone (Used for RNA knockdown)

    Depositor

    Jennifer Doudna

    Article

    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

    Insert

    FLAG-NLS-Csm1;FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; RFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PB-SAM

    Plasmid

    #102559

    Purpose

    Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Inserts

    MS2-P65-HSF1_T2A_Hygro
    dCAS9(D10A, N863A)-VP64_T2A_Blast
    

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9 and N55K in MS2

    Promoter

    CAG and EF1A

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. m168

    Plasmid

    #34886

    Depositor

    Irvin Chen

    Article

    Morizono et al Nat Med. 2005 Mar;11(3):346-52. Epub 200

    Insert

    Sindbis virus envelope protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti-H3.3(K36M)-3AID-HA-2A-mCherryBSD

    Plasmid

    #225699

    Purpose

    Lentiviral expression of AID degron tagged H3.3(K36M) and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (K36M) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mCherry

    Expression

    Mammalian

    Mutation

    K36M

    Promoter

    pEF1A

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. hCRISPRa-v2, genome-wide, 10 sgRNAs/gene

    Pooled Library

    #1000000091

    Purpose

    Genome-wide CRISPR gRNA pooled library for activation of human genes

    Depositor

    Jonathan Weissman

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only