We narrowed to 14,350 results for: cas9

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ALKBH5-dCas9

Plasmid

#134783

Purpose

Expression fusion protein ALKBH5-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Publication

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

ALKBH5 and inactive dCas9

Expression

Mammalian

Promoter

CMV

Available since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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Tet-inducible Luciferase reporter

Plasmid

#64127

Purpose

Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

luciferase

Use

Luciferase

Expression

Mammalian

Promoter

TetOx13-CMV

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pXPR_120

Plasmid

#96917

Purpose

for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR

Depositor

John Doench, David Root

Publication

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Insert

dCas9

Use

Lentiviral

Tags

VPR

Expression

Mammalian

Mutation

D10A, N863A

Promoter

EF1a

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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dxCas(3.7)-VPR

Plasmid

#108383

Purpose

Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus

Depositor

David Liu

Publication

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

dxCas(3.7)-VPR

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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lenti sgRNA(MS2)_puro optimized backbone

Plasmid

#73797

Purpose

optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Publication

Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EF1A

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pHD-DsRed

Plasmid

#51434

Purpose

Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.

Depositor

Kate O'Connor-Giles

Publication

Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

Insert

LoxP-3xP3-DsRed-LoxP

Promoter

3xP3

Available since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

56 Articles

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pLV GG hUbC-dsRED

Plasmid

#84034

Purpose

Contains Golden Gate cassette to express up to four gRNAs and dsRED

Depositor

Charles Gersbach

Publication

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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CMV-hRad51

Plasmid

#125570

Purpose

Expresses the Rad51 protein in mammalian cells under CMV promoter

Depositor

David Liu

Publication

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51 (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCAG-EGxxFP-Cetn1

Plasmid

#50717

Purpose

Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Publication

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Insert

Cetn1 (Cetn1 Mouse)

Use

CRISPR

Expression

Mammalian

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pNW3

Plasmid

#53372

Purpose

Csy4 and Cas9 D10A nickase expression plasmid

Depositor

Keith Joung

Publication

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

Csy4-T2A-Cas9n (D10A)

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FTO-dCas9

Plasmid

#134781

Purpose

Expression fusion protein FTO-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Publication

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

FTO and inactive dCas9

Expression

Mammalian

Promoter

CMV

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDestTol2CG2-U6:gRNA

Plasmid

#63156

Purpose

Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.

Depositor

Leonard Zon

Publication

Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

Type

Empty backbone

Use

CRISPR; Tol2 destination vector for gateway

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pLenti-HF1RA-P2A-GFP-PGK-Puro

Plasmid

#110866

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1RA

Use

Lentiviral

Tags

FLAG

Mutation

R661A, Q695A, Q926A, and NLS sequence at the N-te…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pR26 CAG/GFP Asc

Plasmid

#74285

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI

Depositor

Ralf Kuehn

Publication

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Mouse Targeting

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pMH-Cas9-gate

Plasmid

#113742

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.

Depositor

Magdalena Bezanilla

Publication

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR and Gateway Cloning

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pICH86966::AtU6p::sgRNA_PDS

Plasmid

#46966

Purpose

Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

Depositor

Sophien Kamoun

Publication

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p::sgRNA_PDS

Use

CRISPR; Plant expression

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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NLS-dCas9-trCIB1

Plasmid

#64119

Purpose

Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

dCas9-trCIB1 fusion

Tags

HA and NLS

Expression

Mammalian

Mutation

dCas9 (D10A H840A, catalytically inactive), trCIB…

Promoter

CMV

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA-dCas9-HA

Plasmid

#61355

Purpose

encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

Depositor

Charles Gersbach

Publication

Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

Insert

S.pyogenes dCas9 with c-terminal HA tag

Use

CRISPR and Synthetic Biology

Tags

Flag, HA, and SV40 NLS

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

CMV

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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GAL4UAS-Luciferase reporter

Plasmid

#64125

Purpose

Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

GAL4UAS

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

GAL4UAS

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pDestTol2pA2-U6:gRNA

Plasmid

#63157

Purpose

Destination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.

Depositor

Leonard Zon

Publication

Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

Type

Empty backbone

Use

CRISPR; Tol2 destination vector for gateway

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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