We narrowed to 18,503 results for: REV

1. lenti sgRNA(MS2)_zeo backbone

   Plasmid

   #61427

   Purpose

   3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. mVenus-NES-SspB-MCS-P2A-iLIDcaax

   Plasmid

   #173864

   Purpose

   Mammalian expression of mVenus-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mVenus-NES-SspB-MCS-P2A-iLIDcaax
   

   Tags

   C-terminal CAAX-box and mVenus

   Expression

   Mammalian

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVS1-PC-GCaMP6f

   Plasmid

   #73503

   Purpose

   GCaMP6f knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Insert

   GCaMP6f
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti_dCas9-KRAB-MeCP2

   Plasmid

   #122205

   Purpose

   Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

   Depositor

   Andrea Califano

   Article

   Tan et al bioRxiv 2021.06.28.449297

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   Lentiviral

   Tags

   KRAB-MeCP2

   Expression

   Mammalian

   Mutation

   Cas9 (D10A, H840A)

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase

   Plasmid

   #223747

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   Lambda-PPase
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHage2-EF1a-TERT-IRES-Puro

   Plasmid

   #182590

   Purpose

   expresses TERT in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   hTERT (TERT Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC-BETA

   Plasmid

   #53272

   Purpose

   Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant Cell. 1996 Sep;8(9):1613-26.

   Insert

   crtE, crtY, crtI, crtB
   

   Use

   Low copy number bacterial cloning vector

   Promoter

   endogenous promoters

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. HDAC7 Flag

   Plasmid

   #13824

   Purpose

   Mammalian expression of human histone deacetylase 7 with flag tag

   Depositor

   Eric Verdin

   Article

   Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

   Insert

   HDAC7 (HDAC7 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Available Since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAcGFP1-N1-RvCAHS12-GS

   Plasmid

   #192462

   Purpose

   Expression of RvCAHS12-GS_linker-AcGFP1 in mammalian cells

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

   Insert

   CAHS12
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAcGFP1-N1-RvCAHS8-GS

    Plasmid

    #192461

    Purpose

    Expression of RvCAHS8-GS_linker-AcGFP1 in mammalian cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS8
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. T-2A-EGFP-PGK-Puro

    Plasmid

    #83344

    Purpose

    Donor vector to knocin EGFP into human Brachyury(T) locus

    Depositor

    James Thomson

    Article

    Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

    Insert

    Human Brachyury knockin homology arms (TBXT Human)
    

    Tags

    2A-EGFP

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFlp-Km

    Plasmid

    #222334

    Purpose

    Expresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Inserts

    sacB (sacB B. subtilis)
    flp (flp Budding Yeast)
    cI (cI )
    

    Promoter

    cl, flp, and sacB

    Available Since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. T-STOP sgRNA-1

    Plasmid

    #83346

    Purpose

    The sgRNA at the stop codon of human Brachyury(T) locus

    Depositor

    James Thomson

    Article

    Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

    Insert

    T-STOP sgRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSMART Tmem192-3X HA (targeting vector for genomic tagging)

    Plasmid

    #175777

    Purpose

    pSMART Tmem192-3X HA (targeting vector for genomic tagging)

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. mEGFP-HRas G12V

    Plasmid

    #18666

    Depositor

    Karel Svoboda

    Article

    Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

    Insert

    H-Ras (HRAS Human)
    

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    G12V mutation in HRas

    Available Since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #138149

    Purpose

    Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. tet-pLKO shGATA6 puro

    Plasmid

    #72615

    Purpose

    Expresses an inducible short hairpin targeting human GATA6 sequence

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    short hairpin GATA binding protein 6
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Promoter

    TRE Tight

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICH47811-pAtAct2::Cas9-nls::tSlATPase

    Plasmid

    #160221

    Purpose

    Cas9-nuclear localization, pACT2 promoter, SlATPase terminator transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 2 reverse

    Depositor

    Matias Kirst

    Article

    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

    Insert

    pAtACT2::Cas9-nls::tSlATPase
    

    Use

    Synthetic Biology; Moclo toolkit level 1 acceptor…

    Expression

    Plant

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJR031

    Plasmid

    #198825

    Purpose

    Stable step function ChR2 variant. Activated by bluelight, terminated by green light.

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::ChR2(C128S)-EYFP::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLR5-CAG-44B12mut23(Ser5ph)-SNAP

    Plasmid

    #174877

    Purpose

    PiggyBac transposon vector for expression of RNAPII Ser5ph mintbody

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    RNAPII Ser5ph mintbody-SNAP
    

    Use

    Piggybac vector

    Expression

    Mammalian

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pJR032

    Plasmid

    #198773

    Purpose

    Stabilized step function Opsins ChR2 variant

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::hChR2 (C128S, D156A)-EYFP::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pJR034

    Plasmid

    #198829

    Purpose

    Step-Waveform Inhibitory Channelrhodopsin

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::SwiCHR-TS-EYFP::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pJR033

    Plasmid

    #198822

    Purpose

    Chloride-conducting channelrhodopsin

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::UAS-iC1-c2-TS-EYFP::let-858 3'UTR
    

    Expression

    Worm

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pEGFP.90 beta

    Plasmid

    #108221

    Purpose

    Mammalian expression vector for EGFP fusion to human Hsp90 beta

    Depositor

    Didier Picard

    Article

    Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

    Insert

    EGFP-Hsp90b (HSP90AB1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. pLX-TRE-dCas9-KRAB-MeCP2-BSD

    Plasmid

    #140690

    Purpose

    Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

    Depositor

    Andrea Califano

    Article

    Tan et al bioRxiv 2021.06.28.449297

    Insert

    Cas9m4-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. lenti U6-sgRNA/EF1a-mCherry

    Plasmid

    #114199

    Purpose

    Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    sgRNA scaffold
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pRSET-4xTR-TUBE

    Plasmid

    #110312

    Purpose

    E. coli expression and purification of 4xTR-TUBE

    Depositor

    Yasushi Saeki

    Article

    Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

    Insert

    4xTR-TUBE (UBQLN1 Human)
    

    Tags

    N-terminal His6-T7 tag

    Expression

    Bacterial

    Mutation

    All Arg residues in the UBA domain are mutated to…

    Promoter

    T7

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pRMC2-msfGFP

    Plasmid

    #194913

    Purpose

    Tetracycline inducible expression of msfGFP in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno Sequence followed by monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Expression

    Bacterial

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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29. pTagBFP-C1-FKBP-PIP5K1C-HD-EE

    Plasmid

    #220090

    Purpose

    Recruitable PIP5K to make PIP2

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    PIP5K1C (PIP5K1C Human)
    

    Tags

    TagBFP-FKBP1A(3-108)

    Expression

    Mammalian

    Mutation

    amino acids 1-635; D101R, R304D, R445E, K446E

    Promoter

    CMV

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pSpCas9_T2A_mCherry sgAAVS1

    Plasmid

    #192480

    Purpose

    All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A mCherry

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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