Addgene

We narrowed to 11,161 results for: ENA

Showing: 1751 - 1800 of 11161 results

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VinculinTS-I997A

Plasmid

#111828

Purpose

Vinculin tension sensor with point mutation affecting ability to bind actin

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

VinculinTS (I997A)

Use

Tags

Expression

Mammalian

Mutation

changed isoleucine 997 to alanine

Promoter

CMV

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET303-colE1TR

Plasmid

#155181

Purpose

Expresses colicin E1 residues 1-364

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

colicin E1 residues 1-364

Use

Tags

C-terminal 6x Histidine tag

Expression

Bacterial

Mutation

truncation of colicin E1 including 1-364

Promoter

T7

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-pDisplay-Halo

Plasmid

#223497

Purpose

Expression of HaloTags to outer surface in mammalian cells

Depositor

James Frank

Article

Tobias et al Chem Sci. 2021 Sep 15;12(40):13506-13512

Insert

HaloTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pKO (Apr)

Plasmid

#110088

Purpose

Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Tags

Expression

Mutation

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTRANS_260d

Plasmid

#91126

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

PvUbi2

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-pDisplay-SNAP

Plasmid

#223495

Purpose

Expression of SNAP-tags to outer surface in mammalian cells

Depositor

James Frank

Article

Tobias et al Chem Sci. 2021 Sep 15;12(40):13506-13512

Insert

SNAP-tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-SnoopTagJr-MBP

Plasmid

#105628

Purpose

Plasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigase

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

SnoopTagJr-MBP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS5390-CRISPRai-URA3

Plasmid

#182829

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - URA3 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pOSML-Cox2-GFP

Plasmid

#203711

Purpose

Overexpression of COX2-GFP fusion

Depositor

Timothy Hla

Article

Trifan et al J Biol Chem. 1999 Nov 26;274(48):34141-7

Insert

Cox2-GFP (PTGS2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

WT

Promoter

SV40

Available since

June 19, 2024

Availability

Academic Institutions and Nonprofits only

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pWS5398-CRISPRai-HygR

Plasmid

#182837

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - HygR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_449

Plasmid

#179093

Purpose

nuclease Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

SpG

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR Clover-VISH2

Plasmid

#188629

Purpose

Fluorescent reporter for SLP76/VISH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

VISH2

Use

Lentiviral

Tags

Clover

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-RagA(T21N)

Plasmid

#99711

Purpose

Mammalian expression of HA-RagA(T21N)

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Use

Tags

HA

Expression

Mutation

T21N

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_100

Plasmid

#91198

Purpose

Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKO (Hyg)

Plasmid

#110087

Purpose

Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Tags

Expression

Mutation

Promoter

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCpf1-sp

Plasmid

#122186

Purpose

Bacterial genome editing plasmid with spectinomycin resistance marker

Depositor

Cheng-Cai Zhang

Article

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRANS_150

Plasmid

#91205

Purpose

Transformation, Type: non-T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
YCplac33-NAT

Plasmid

#140594

Purpose

Expression in S. cerevisiae

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_A3001

Plasmid

#91042

Purpose

Module A, Promoter: 35S, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Tags

Expression

Mutation

Promoter

35S

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
paavCAG-pre-mGRASP-2A-dTomato

Plasmid

#51902

Purpose

pre-2Ato

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

presynaptic mGRASP

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_B0000

Plasmid

#91058

Purpose

Module B, Promoter: none, Gene: none, Terminator: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-jAspSnFR3-mRuby3-NLS

Plasmid

#203494

Purpose

Nuclear directed jAspSnFR3-mRuby3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3-NLS

Use

Lentiviral

Tags

Nuclear localization signal and mRuby3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 HA-RagC(Q120L)

Plasmid

#99720

Purpose

Mammalian expression of HA-RagC(Q120L)

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Use

Tags

HA

Expression

Mutation

Q120L

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
ChlorON-3

Plasmid

#193479

Purpose

Turn-on fluorescent sensor for chloride

Depositor

Sheel Dodani

Article

Tutol et al ACS Cent Sci. 2023 Dec 18;10(1):77-86. d

Insert

ChlorON-3

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRL-Vinculin-Venus

Plasmid

#111833

Purpose

Vinculin (chicken) tagged with VenusA206K at the C-terminus in lentiviral expression vector

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

Vinculin-Venus (VCL Chicken)

Use

Lentiviral

Tags

VenusA206K

Expression

Mutation

Promoter

CMV

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFG036 + pFG051

Plasmid

#137997

Purpose

Transposon mutagenesis plasmid and the cI repressor expression plasmid

Depositor

Sébastien Rodrigue

Article

Neil et al Commun Biol. 2020 Sep 22;3(1):523. doi:

Insert

Tn5 tnp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL358

Plasmid

#169147

Purpose

Encodes OptoQ-AMP1

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pF-N-HiBiT-template

Plasmid

#200882

Purpose

template for N-terminal HiBIT-tagging

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

HiBIT-linker

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL513

Plasmid

#169153

Purpose

Encodes OptoQ-INVRT1

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
qMaLioffG

Plasmid

#212825

Purpose

green fluorescent FLIM indicator for endogenous ATP imaging

Depositor

Tetsuya Kitaguchi

Article

Arai et al bioRxiv 2023.08.29.555275

Insert

qMaLioffG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
sgDonor_lentiGuide

Plasmid

#184168

Purpose

LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Insert

sgDonor

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS5397-CRISPRai-NatR

Plasmid

#182836

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - NatR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-HxB.L

Plasmid

#65414

Purpose

contains complete cDNA for TNC large splice variant flanked by NotI-KpnI

Depositor

Harold Erickson

Article

Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

Insert

TNC large splice variant (TNC Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRESNEO-Scambio-HA

Plasmid

#33354

Depositor

John Groffen, Nora Heisterkamp

Article

Curtis et al Mol Cancer. 2004 Apr 23;3:10.

Insert

Scambio (ARHGEF40 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
RFP Dynamin2 Wild Type

Plasmid

#128152

Purpose

Expression of Dynamin 2

Depositor

Jennifer Lippincott-Schwartz

Article

Chua et al Proc Natl Acad Sci U S A. 2009 Nov 30. (

Insert

Dynamin 2 (Dnm2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5 Flag-RagA(Q66L)

Plasmid

#99716

Purpose

Mammalian expression of Flag-RagA(Q66L)

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Use

Tags

Flag

Expression

Mutation

Q66L

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
paavCAG-JxOFF-pre-mGRASP-mCerulean

Plasmid

#51900

Purpose

JxOFF-pre

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

presynaptic mGRASP

Use

AAV and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pPyCAG-Nanog-IP

Plasmid

#13838

Depositor

Shinya Yamanaka

Article

Mitsui et al Cell. 2003 May 30. 113(5):631-42.

Insert

Nanog (Nanog Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS5396-CRISPRai-KanR

Plasmid

#182835

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - KanR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_A0000

Plasmid

#90997

Purpose

Module A, Promoter: none, Gene: none, Terminator: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_speG_Cterm_His

Plasmid

#220483

Purpose

heterologous expression for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL728

Plasmid

#169152

Purpose

Encodes OptoQ-AMP6

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSDH61

Plasmid

#139033

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

epdz (no STOP codon)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pNRB150

Plasmid

#36451

Depositor

Douglas Bishop

Article

Hong et al J Biol Chem. 2001 Nov 9;276(45):41906-12

Insert

DMC1 (DMC1 Budding Yeast)

Use

Tags

6His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pS24·Pm-GFP

Plasmid

#122594

Purpose

Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

Depositor

Pablo Ivan Nikel

Article

Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

Pm promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pm

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_PA4114_Cterm_His

Plasmid

#220484

Purpose

heterologous expression of PA4114 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Use

Tags

6xHs

Expression

Bacterial

Mutation

none

Promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pYC56

Plasmid

#63910

Purpose

Recipient integrative vector to construct mCherry tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_Sox9_enh_349_Citrine

Plasmid

#130594

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox9_enh_349

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC_C_AOX

Plasmid

#111661

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

AOX

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CSII-TK-MCS

Plasmid

#203597

Purpose

Empty lentiviral vector to express protein in mammalian cells cells.

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TK

Available since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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