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AIRAP 2: AIRAP(m1.9-T7)pGEX.KG

Plasmid

#21792

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

AIRAP (Zfand2a Mouse)

Tags

GST

Expression

Bacterial

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO SSA1

Plasmid

#19462

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

SSA1 (SSA1 Budding Yeast)

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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M29 NacMS+LEF>Nac

Plasmid

#17179

Depositor

Randall Moon

Article

Dorsky et al Genes Dev. 2000 Jan 15;14(2):158-62.

Insert

nacre promoter driving nacre cDNA (mitfa Zebrafish)

Mutation

All three Tcf/Lef sites are mutated, but 5 LEF si…

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
xfz8 probe/pBS

Plasmid

#16323

Depositor

Peter Klein

Article

Deardorff et al Development. 1998 Jul . 125(14):2687-700

Insert

frizzled-8 (fzd8.S Frog)

Expression

Bacterial

Mutation

Base pairs 1159-3240 of xfz8 cDNA

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pA9HD

Plasmid

#8588

Depositor

Cynthia Wolberger

Article

LaRonde-LeBlanc et al Genes Dev 2003 Aug 15;17(16):2060-72.

Insert

HoxA9 190-271with hexapeptide (HOXA9 Human)

Tags

His

Expression

Bacterial

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
pV1338

Plasmid

#111443

Purpose

Solo vector pV1326 + sgScADE2

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgScADE2

Use

CRISPR

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pCKTRBS-CbnR-ABE44898-OD

Plasmid

#102925

Purpose

pCKTRBS-OD derivative carrying CbnR-ABE44898-OD chimera, also known as ChBz01

Depositor

George Church

Article

Juarez et al Nat Commun. 2018 Aug 6;9(1):3101. doi: 1

Insert

CbnR-ABE44898-OD (ChTFBz01)

Promoter

PtetO

Availability

Academic Institutions and Nonprofits only

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pCKTRBS-LmrR-BzdB1_nSP

Plasmid

#102926

Purpose

pCKTRBS derivative carrying LmrR-BzdB1_nSP chimera, also known as ChBz02

Depositor

George Church

Article

Juarez et al Nat Commun. 2018 Aug 6;9(1):3101. doi: 1

Insert

LmrR-BzdB1_nSP

Expression

Bacterial

Promoter

PtetO

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-5SA/S94A

Plasmid

#33103

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

YAP (YAP1 )

Tags

Flag

Expression

Mammalian

Mutation

S61A, S94A, S109A, S127A, S164A, S381A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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mCardinal-C1

Plasmid

#54799

Purpose

Localization: C1 Cloning Vector, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Tags

mCardinal

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pET22b-His6-UBCH7

Plasmid

#212711

Purpose

Bacterial expression of His6-UBCH7

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

UBCH7

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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mScarletI3-6DG5-MAPPER

Plasmid

#222421

Purpose

To express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

MAPPER

Tags

mScarletI3-6DG5

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMU-PERr

Plasmid

#61202

Purpose

Fluorescent reporter for peroxisomes mCherry-SKL expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

mCherry-SKL

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52

Plasmid

#98678

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_eeBxb1_Int_2x_NPL_c-term_NLS_pTwist

Plasmid

#244901

Purpose

Expression of eeBxb1

Depositor

Andrew Bassett

Article

Migliori et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

eeBxb1

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pQCXIP-GFP1-10

Plasmid

#68715

Purpose

Split GFP assay

Depositor

Yutaka Hata

Article

Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

Insert

GFP1-10

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2016

Availability

Academic Institutions and Nonprofits only

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scFv-GCN4-sfGFP-TET1CD

Plasmid

#184439

Purpose

TET1 catalytic domain fused with sfGFP and scFv against GCN4

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

scFv-GCN4-sfGFP-TET1CD

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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attB-GA-mNeonGreen-linker-attB-GT::pUC-GW-Kan

Plasmid

#244898

Purpose

Recombinase-mediated integration

Depositor

Andrew Bassett

Article

Migliori et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

mNeonGreen

Use

Unspecified

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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px459 VQR

Plasmid

#101715

Purpose

sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VQR (D1135V, R1335Q and T1337R)

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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px459 VRER

Plasmid

#101716

Purpose

sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E and T1337R)

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-NLS-HA-Dre

Plasmid

#51272

Purpose

Component of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

Dre

Tags

NLS-HA

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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attB-CT-mCherry-linker-attB-AG::pUC-GW-Kan

Plasmid

#244900

Purpose

Recombinase-mediated integration

Depositor

Andrew Bassett

Article

Migliori et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

mCherry

Use

Unspecified

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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AW01_pU.CAG.eSpCas9(1.1).rBGpA

Plasmid

#199255

Purpose

Expression construct encoding the high specificity eSpCas9(1.1) nuclease

Depositor

Manuel Goncalves

Article

Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

Insert

High specificity eSpCas9(1.1) nuclease

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

K848A K1003A R1060A

Promoter

CAG promoter

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGreenPuro-shRNA-Stx3S-C4

Plasmid

#99742

Purpose

3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.

Depositor

Thomas Weimbs

Article

Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

Insert

shRNA against Stx3S

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2

Plasmid

#84603

Purpose

Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

Depositor

James Chen

Article

Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

Insert

mCherry

Use

Zebrafish transgenesis

Tags

ODC1 destabilizing element

Promoter

8xGliBS-delta-crystallin minimal promoter

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1

Plasmid

#108127

Purpose

Recruitable SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L (SACM1L Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1C389S

Plasmid

#108128

Purpose

Recruitable inactive SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(C389S)

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-Mem

Plasmid

#55779

Purpose

This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mCherry.

Depositor

Catherine Berlot

Article

Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

Insert

mCherry-Mem

Tags

The N-terminal 20 residues of neuromodulin are fu…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113341

Purpose

Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

VP64-dCas9-VP64-T2A-MS2-p65-HSF1

Use

Piggybac

Expression

Mammalian

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pHCKan-phoAp-GFPuv

Plasmid

#139072

Purpose

Low phosphate inducible promoter phoAp for GFP expression

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

phoAp-GFPuv

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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