We narrowed to 15,995 results for: LEA

1. pH6Z-812

   Plasmid

   #85258

   Purpose

   Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

   Depositor

   Philip Pellett

   Article

   Lindquester et al Arch Virol. 1996;141(2):367-79.

   Insert

   fragment of the human herpesvirus 6B strain Z29 genome
   

   Use

   Cloning vector

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pH6Z-607

   Plasmid

   #85246

   Purpose

   Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

   Depositor

   Philip Pellett

   Article

   Lindquester et al Arch Virol. 1996;141(2):367-79.

   Insert

   fragment of the human herpesvirus 6B strain Z29 genome
   

   Use

   Cloning vector

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pH6Z-606

   Plasmid

   #85245

   Purpose

   Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

   Depositor

   Philip Pellett

   Article

   Lindquester et al Arch Virol. 1996;141(2):367-79.

   Insert

   fragment of the human herpesvirus 6B strain Z29 genome
   

   Use

   Cloning vector

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pH6Z-605

   Plasmid

   #85244

   Purpose

   Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

   Depositor

   Philip Pellett

   Article

   Lindquester et al Arch Virol. 1996;141(2):367-79.

   Insert

   fragment of the human herpesvirus 6B strain Z29 genome
   

   Use

   Cloning vector

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pH6Z-603

   Plasmid

   #85242

   Purpose

   Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

   Depositor

   Philip Pellett

   Article

   Lindquester et al Arch Virol. 1996;141(2):367-79.

   Insert

   fragment of the human herpesvirus 6B strain Z29 genome
   

   Use

   Cloning vector

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pH6Z-602

   Plasmid

   #85241

   Purpose

   Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

   Depositor

   Philip Pellett

   Article

   Lindquester et al Arch Virol. 1996;141(2):367-79.

   Insert

   fragment of the human herpesvirus 6B strain Z29 genome
   

   Use

   Cloning vector

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pH6Z-604

   Plasmid

   #85243

   Purpose

   Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

   Depositor

   Philip Pellett

   Article

   Lindquester et al Arch Virol. 1996;141(2):367-79.

   Insert

   fragment of the human herpesvirus 6B strain Z29 genome
   

   Use

   Cloning vector

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pH6Z-232

   Plasmid

   #85238

   Purpose

   Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

   Depositor

   Philip Pellett

   Article

   Lindquester et al Arch Virol. 1996;141(2):367-79.

   Insert

   fragment of the human herpesvirus 6B strain Z29 genome
   

   Use

   Cloning vector

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pH6Z-227

   Plasmid

   #85237

   Purpose

   Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

   Depositor

   Philip Pellett

   Article

   Lindquester et al Arch Virol. 1996;141(2):367-79.

   Insert

   fragment of the human herpesvirus 6B strain Z29 genome
   

   Use

   Cloning vector

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pH6z-225

    Plasmid

    #85236

    Purpose

    Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

    Depositor

    Philip Pellett

    Article

    Lindquester et al Arch Virol. 1996;141(2):367-79.

    Insert

    fragment of the human herpesvirus 6B strain Z29 genome
    

    Use

    Cloning vector

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pH6Z-207

    Plasmid

    #85234

    Purpose

    Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

    Depositor

    Philip Pellett

    Article

    Lindquester et al Arch Virol. 1996;141(2):367-79.

    Insert

    fragment of the human herpesvirus 6B strain Z29 genome
    

    Use

    Cloning vector

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pH6Z-202

    Plasmid

    #85232

    Purpose

    Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

    Depositor

    Philip Pellett

    Article

    Lindquester et al Arch Virol. 1996;141(2):367-79.

    Insert

    fragment of the human herpesvirus 6B strain Z29 genome
    

    Use

    Cloning vector

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pH6Z-201

    Plasmid

    #85231

    Purpose

    Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

    Depositor

    Philip Pellett

    Article

    Lindquester et al Arch Virol. 1996;141(2):367-79.

    Insert

    fragment of the human herpesvirus 6B strain Z29 genome
    

    Use

    Cloning vector

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pH6Z-203

    Plasmid

    #85233

    Purpose

    Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

    Depositor

    Philip Pellett

    Article

    Lindquester et al Arch Virol. 1996;141(2):367-79.

    Insert

    fragment of the human herpesvirus 6B strain Z29 genome
    

    Use

    Cloning vector

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pH6Z-109

    Plasmid

    #85230

    Purpose

    Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

    Depositor

    Philip Pellett

    Article

    Lindquester et al Arch Virol. 1996;141(2):367-79.

    Insert

    fragment of the human herpesvirus 6B strain Z29 genome
    

    Use

    Cloning vector

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pH6Z-106

    Plasmid

    #85228

    Purpose

    Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

    Depositor

    Philip Pellett

    Article

    Lindquester et al Arch Virol. 1996;141(2):367-79.

    Insert

    fragment of the human herpesvirus 6B strain Z29 genome
    

    Use

    Cloning vector

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MS4A1 RFP (805)

    Plasmid

    #62009

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

    Insert

    MS4A1
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    pgk

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

18. γA3/N-GFP

    Plasmid

    #33113

    Depositor

    Greg Phillips

    Article

    O'Leary et al Mol Biol Cell. 2011 Sep 14.

    Insert

    Pcdh-γA3 (Pcdhga3 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain has been replaced with the N-c…

    Promoter

    CMV

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

19. Δ741-752Δconst-GFP

    Plasmid

    #33120

    Depositor

    Greg Phillips

    Article

    O'Leary et al Mol Biol Cell. 2011 Sep 14.

    Insert

    Pcdh-γA3 (Pcdhga3 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of aa 741-752 along with the entire cons…

    Promoter

    CMV

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

20. TEV Protease-mCherry

    Plasmid

    #243793

    Purpose

    Encodes for mammalian expression of TEV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

    Depositor

    Cole DeForest

    Article

    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

    Insert

    TEV protease
    

    Expression

    Mammalian

    Available Since

    Oct. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTetOne-mCherry-cNLS

    Plasmid

    #138527

    Purpose

    Inducible expression of mCherry with nuclear localization signal

    Depositor

    Christopher Grigsby, Ana Teixeira

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    mCherry
    

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Promoter

    TRE3Gs

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET30b-5M SrtA with TEV site

    Plasmid

    #86962

    Purpose

    Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

    Depositor

    Ron Bose

    Article

    Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

    Insert

    S.aureus SrtA 5M
    

    Tags

    6x Histidine tag and TEV site

    Expression

    Bacterial

    Mutation

    P94R, D160N, D165A, K190E, K196T

    Promoter

    T7

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Cp PS Intein tTA

    Plasmid

    #242026

    Purpose

    Blue-light-controlled release of tTA transactivator from the cytoplasm to the nucleus.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    Cp PS Intein tTA
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pD-MSP1D1

    Plasmid

    #161607

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    D-MSP1D1
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1; all tryptophans were replaced b…

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. HER2Nb-C AND (A OR T)-SpyTag

    Plasmid

    #243790

    Purpose

    Expresses multifunctional construct enabling the AND(OR)-gated release of a HER2 Nanobody from materials in response to HRV-3C AND (sortase eSrtA(2A9) OR TEV) treatment.

    Depositor

    Cole DeForest

    Article

    Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

    Insert

    HER2 nanobody
    

    Tags

    6x His tag and SsrA

    Expression

    Bacterial

    Available Since

    Oct. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-mCherry-Rheb

    Plasmid

    #192436

    Purpose

    Encodes mCherry-tagged wild-type Rheb.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-Rheb (RHEB Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNuc-cis

    Plasmid

    #202796

    Purpose

    RK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killing

    Depositor

    David Edgell

    Article

    Hamilton et al Nat Commun. 2019 Oct 4;10(1):4544. doi:

    Insert

    TevSpCas9, sgRNA cassette, oriT, CenArsHis
    

    Expression

    Bacterial

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUD-MSP1D1

    Plasmid

    #161608

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    UD-MSP1D1
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1; all tryptophans and tyrosines w…

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-ZFN-AAVS1

    Plasmid

    #89707

    Purpose

    Mammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locus

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Viruses. 2017 Nov 2;9(11). pii: v9110325

    Insert

    ZFN-AAVS1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Tol2_B-act_dCas9-Vp64-T2A-Citrine

    Plasmid

    #200248

    Purpose

    Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    dCas9-Vp64-T2A-Citrine
    

    Use

    Zebrafish expression

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGL3-mArg1 promoter/enhancer -31/-3810

    Plasmid

    #34571

    Depositor

    Peter Murray

    Article

    Pauleau et al J Immunol. 2004 Jun 15;172(12):7565-73.

    Insert

    Arg1 promoter/enhancer -31/-3810 (Arg1 Mouse)
    

    Use

    Luciferase

    Tags

    Firefly Luciferase

    Available Since

    Jan. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCDH-NLS-copGFP-EF1-BlastiS

    Plasmid

    #132772

    Purpose

    Spills into the cytoplasm during nuclear envelope rupture.

    Depositor

    Jan Lammerding

    Article

    Denais et al Science. 2016 Apr 15;352(6283):353-8. do

    Insert

    NLS-copGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. SpyTag-sfGFP

    Plasmid

    #184226

    Purpose

    Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    SpyTag-sfGFP
    

    Tags

    His6, SpyTag, and TEV protease cleavage site

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA-NES-PhoCl-mCherry

    Plasmid

    #87690

    Purpose

    Express NES-PhoCl-mCherry in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NES-PhoCl-mCherry
    

    Tags

    nuclear export signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGL3-mArg1 base promoter -31/-2365

    Plasmid

    #34570

    Depositor

    Peter Murray

    Article

    Pauleau et al J Immunol. 2004 Jun 15;172(12):7565-73.

    Insert

    Arg1 base promoter -31/-2365 (Arg1 Mouse)
    

    Use

    Luciferase

    Tags

    Firefly Luciferase

    Available Since

    Dec. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pAcGFP-MALAT1M

    Plasmid

    #120382

    Purpose

    Reporter for RNA nuclear localization

    Depositor

    Igor Ulitsky

    Article

    Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

    Insert

    MALAT1 (MALAT1 Human)
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHsp70-Cas9

    Plasmid

    #45945

    Purpose

    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

    Depositor

    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

    Article

    Gratz et al Genetics. 2013 May 24.

    Insert

    Codon optimized Cas9
    

    Use

    CRISPR

    Tags

    3x FLAG

    Mutation

    Silent change from C to A at bp 834 of insert

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTetOne-tdTomato-cNLS

    Plasmid

    #138529

    Purpose

    Inducible expression of tdTomato with nuclear localization signal

    Depositor

    Christopher Grigsby, Ana Teixeira

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    tdTomato
    

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Promoter

    TRE3Gs

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSQT834

    Plasmid

    #53371

    Purpose

    Csy4 and Cas9 nuclease expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Csy4-T2A-Cas9-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. NET47 pEGFP-N2 (593)

    Plasmid

    #61986

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET47 (TM7SF2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

41. pCAG-T3-hCAS-pA

    Plasmid

    #48625

    Purpose

    Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii, Kunihiko Naito

    Article

    Fujii et al Nucleic Acids Res. 2013 Aug 30.

    Insert

    Codon optimized Cas9
    

    Use

    CRISPR

    Tags

    FLAG, NLS, and polyA

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA-NLS-PhoCl-mCherry

    Plasmid

    #87691

    Purpose

    Express NLS-PhoCl-mCherry in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NLS-PhoCl-mCherry
    

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. M-NMn-VP64

    Plasmid

    #48676

    Purpose

    Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Mutation

    nuclease-null

    Promoter

    CMV

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA3-mCherry-TSC2

    Plasmid

    #192440

    Purpose

    Encodes mCherry-tagged wild-type TSC2 isoform 5.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2 (TSC2 Human)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pUB-MSP1D1

    Plasmid

    #161609

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    UD-MSP1D1
    

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1 ( Q138W, L181W, Y236W)

    Promoter

    T7

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCL1032

    Plasmid

    #217962

    Purpose

    Mammalian expression of NASP ('s' isofrom) fused at its N-terminus to eGFP with a TEV protease site separating the two. Puromycin resistance is expressed from a downstream IRES.

    Depositor

    Andrew Bowman

    Article

    Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

    Insert

    NASP (NASP Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. M-ST1n-VP64

    Plasmid

    #48675

    Purpose

    Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Mutation

    nuclease-null (D9A D598A H599A N622A)

    Promoter

    CMV

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. AAV-H2GH

    Plasmid

    #182202

    Purpose

    Adeno-associated viral vector to express histone2B-HaloTag-GFP fusion, a nuclear-localized Halotag-GFP fusion construct

    Depositor

    Joshua Kritzer

    Article

    Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

    Insert

    histone2B-HaloTag-GFP
    

    Use

    AAV

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCas1_Cas2/3

    Plasmid

    #89240

    Purpose

    Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1

    Depositor

    Blake Wiedenheft

    Article

    Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

    Insert

    Cas1 and Cas2/3
    

    Tags

    Strep II tag on Cas1

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. M-SPn-VP64

    Plasmid

    #48674

    Purpose

    Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Mutation

    nuclease-null

    Promoter

    CMV

    Available Since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only