161,398 results

1. pCS2-XTbx5-EnR

   Plasmid

   #117249

   Purpose

   mRNA synthesis

   Depositor

   Gerald Thomsen

   Article

   Horb et al Development. 1999 Apr;126(8):1739-51.

   Insert

   Tbx5-EnR (tbx5-b Frog)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   chimeric protein with engrailed repressor domain…

   Promoter

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. SuperNova/pRSETB

   Plasmid

   #53234

   Purpose

   monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

   Depositor

   Takeharu Nagai

   Article

   Takemoto et al Sci Rep. 2013;3:2629. doi: 10.1038/srep0

   Insert

   monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
   

   Use

   Tags

   6xHis, T7 tag, and Xpress tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. 2C-3XtbGFP-PEST

   Plasmid

   #69072

   Purpose

   Expresses 3XturboGFP-NLS-PEST under MERVL_LTR

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

   Insert

   turboGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. iLID-mCl3-MAP7N

   Plasmid

   #190167

   Purpose

   Expresses iLID-mCl3-MAP7N, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

   Depositor

   Anna Akhmanova

   Article

   Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

   Insert

   iLID-mClover3-MAP7N
   

   Use

   Tags

   iLID-mClover3

   Expression

   Mammalian

   Mutation

   aa 1-309 from human MAP7 delta175-213

   Promoter

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCherry-C1-mTrim21ΔPRYSPRY

   Plasmid

   #105518

   Purpose

   Expression of mCherry-tagged mouse Trim21 with a deleted PRYSPRY domain in mammalian cells

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   deletion of PRYSPRY domain (aa1-281)

   Promoter

   CMV

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAIP4_1UXZ

   Plasmid

   #234166

   Purpose

   Heterologous protein expression of cellulase B in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1UXZ
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. PGK-DTAG-CTERM-GFP

   Plasmid

   #185770

   Purpose

   Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

   Depositor

   Alessandra Ianari, William Sellers

   Article

   Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   V5, dTAG

   Expression

   Mutation

   none

   Promoter

   PGK

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti-dCas9-VP64-BFP

   Plasmid

   #196711

   Purpose

   CRISPR-activation. dCas9-VP64 with TagBFP for sorting

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-VP64-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSC-humanADAR1

   Plasmid

   #198235

   Purpose

   Expresses human ADAR1 in yeast

   Depositor

   Brenda Bass

   Article

   Eggington et al Nat Commun. 2011;2:319. doi: 10.1038/nco

   Insert

   human ADAR1 (ADAR Human)
   

   Use

   Tags

   His10_TEV

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HA-RIPK3

    Plasmid

    #78804

    Purpose

    To overexpress RIPK3 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQ203 (pMDC32-Ubi1)

    Plasmid

    #86207

    Purpose

    Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Type

    Empty backbone

    Use

    Gateway compatible attr1-attr2 destination vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin 1 (ZmUbi1)

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

    Plasmid

    #158962

    Purpose

    Cre-dependent Cas9-GFP expression

    Depositor

    Michael Brand

    Article

    Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

    Inserts

    hsp70l promoter
    floxed DsRed cassette
    Cas9-GFP
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVS2

    Plasmid

    #33144

    Depositor

    Michael Rosbash

    Article

    Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

    Inserts

    LacO (256 copies)
    GAL-GFP-kd200-GALpA reporter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    GFP is mutated such that amino acids 64-73 (FSYGV…

    Promoter

    bidirectional GAL1-GAL10 promoter

    Available since

    Dec. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pKS071 - pCAGGS-3XFLAG-mKate2-(human)PDS5A-bpA

    Plasmid

    #156449

    Purpose

    For transient expression of human PDS5A tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    human PDS5A (PDS5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDXinit

    Plasmid

    #110101

    Purpose

    E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phages

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Str-KDEL_neomycin

    Plasmid

    #65306

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
    

    Use

    Tags

    KDEL motif and Signal Sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV pmSyn1-EBFP-Cre

    Plasmid

    #51507

    Purpose

    Can be used to generate AAV virus that will express Cre in neurons from the synapsin promoter

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Has Service

    AAV5

    Insert

    EBFP-Cre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pmSyn1

    Available since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-OTp-GCaMP6s

    Plasmid

    #192945

    Purpose

    To drive GCaMP6s under the control of mouse oxytocin promoter

    Depositor

    Kazunari Miyamichi

    Article

    Yaguchi et al PLoS One. 2023 May 10;18(5):e0285589. do

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FLEX-P301L Tau

    Plasmid

    #176267

    Purpose

    This AAV plasmid vector expresses human 2N4R microtubule-associated protein tau with P301L mutation.

    Depositor

    Tsuneya Ikezu

    Article

    You et al Neuroscience. 2019 Dec 1;422:65-74. doi:

    Insert

    Flex-P301L tau (MAPT )
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLB

    Plasmid

    #11619

    Purpose

    pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.

    Depositor

    Stephan Kissler

    Article

    Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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21. Proinsulin-NanoLuc in pLX304

    Plasmid

    #62057

    Purpose

    Luminescent reporter of insulin secretion

    Depositor

    David Altshuler

    Article

    Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

    Insert

    Proinsulin-NanoLuc (Ins2 Synthetic, Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Inserted NanoLuc into C-peptide of mouse Ins2 cod…

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. S1m tagged DANCR

    Plasmid

    #138585

    Purpose

    For in vivo tagging

    Depositor

    Yin-Yuan Mo

    Article

    Hu et al Cell Death Differ. 2019 Dec 5. pii: 10.1

    Insert

    DANCR (DANCR Human)
    

    Use

    Lentiviral

    Tags

    S1m tag

    Expression

    Mutation

    Promoter

    MSCV

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid

    #104909

    Purpose

    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEFBOS_CreIRESpuro

    Plasmid

    #183812

    Purpose

    Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter

    Depositor

    Ed Stanley

    Article

    Davis et al Nat Protoc. 2008;3(10):1550-8. doi: 10.1

    Insert

    Cre recombinase
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pTEF:ATP

    Plasmid

    #92179

    Purpose

    HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Insert

    ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
    

    Use

    Integration and expression into yeast.

    Tags

    Yeast codon optimized mseCFP (donor) and cp173-mV…

    Expression

    Yeast

    Mutation

    Promoter

    TEF1p

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLPT41

    Plasmid

    #85524

    Purpose

    Titration sponge for TetR

    Depositor

    Johan Paulsson

    Article

    Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PLtet

    Available since

    April 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. pHSB04X

    Plasmid

    #117258

    Purpose

    Genome editing for Lactobacillus brevis ATCC367

    Depositor

    Sheng Yang

    Article

    Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

    Insert

    Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA/Myc TCF4

    Plasmid

    #16512

    Depositor

    Bert Vogelstein

    Article

    Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

    Insert

    TCF4 (TCF7L2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1 FLAG-YY1

    Plasmid

    #104396

    Purpose

    FLAG-YY1

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    YY1
    

    Use

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s (AAV5)

    Viral Prep

    #105715-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s (#105715). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s plasmid DNA. EF1a-driven, Cre-dependent GCaMP6s fused to the presynaptic protein synaptophysin. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Rylan Larsen

    Promoter

    Ef1a, Human elongation factor-1 alpha

    Tags

    None

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only