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pMP71-BFP

Plasmid

#141355

Purpose

pMP71 expression vector containing BFP

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

tagBFP

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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AiP13152 - pAAV-AiE0050m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3152)

Plasmid

#224056

Purpose

AiE0050m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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PPRE-H2B-eGFP

Plasmid

#84393

Purpose

Fluorescent reporter for PPARgamma activity

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

Insert

PPARG response element (PPARG Human)

Tags

eGFP

Expression

Mammalian

Promoter

PPRE

Available Since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPR-A

Plasmid

#89119

Purpose

mammalian expression of full-length human progesterone receptor-A, equivalent to human PR-B amino acids 165-933

Depositor

Elizabeth Wilson

Article

Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

Insert

human progesterone receptor-A (PGR Human)

Expression

Mammalian

Mutation

human progesterone receptor-A, equivalent to huma…

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-nsp12

Plasmid

#168909

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-nsp12 (ORF1ab )

Use

Gateway Cloning

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX SC4MOL

Plasmid

#27983

Depositor

Igor Astsaturov

Insert

SC4MOL (MSMO1 Human)

Use

Lentiviral

Tags

HA and MYC

Expression

Mammalian

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
pHR-EF1a-Archon1-KGC-EGFP-ER2

Plasmid

#108425

Purpose

Lentivirus production plasmid encoding for Archon1 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-EGFP-ER2

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-TRIM27

Plasmid

#23607

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TRIM27 (TRIM27 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCerulean-N1 Cyclin B1 (1147)

Plasmid

#61852

Purpose

mammalian expression of Cyclin B1 fused to Cerulean

Depositor

Jonathon Pines

Insert

Cyclin B1 (CCNB1 Human)

Tags

Cerulean

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
CRY2olig-NONO

Plasmid

#218922

Purpose

Expression of CRY2olig-mCherry fused to NONO

Depositor

Tatyana Shelkovnikova

Insert

CRY2olig-mCherry tagged NONO

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe N-Ras 61K

Plasmid

#12543

Depositor

Channing Der

Article

Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

Insert

NRas 61K (NRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

61K constitutively activated.

Available Since

Sept. 8, 2006

Availability

Academic Institutions and Nonprofits only

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pDONR223_RPS6KB1_WT

Plasmid

#82161

Purpose

Gateway Donor vector containing RPS6KB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RPS6KB1 (RPS6KB1 Human)

Use

Gateway Cloning

Mutation

P262H

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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JAK2 (V617F)-pcw107-V5

Plasmid

#64610

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

JAK2 (JAK2 Human)

Use

Lentiviral

Tags

V5

Mutation

V617F

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pET22b_EcSSB_1

Plasmid

#78203

Purpose

Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

Depositor

Martin Webb

Article

Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1

Insert

ssb (G26C)

Expression

Bacterial

Mutation

G26C (please see depositor comment below)

Promoter

T7

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe puro IRES-EGFP

Plasmid

#14430

Depositor

L. Miguel Martins

Has Service

Cloning Grade DNA

Insert

IRES-EGFP

Use

Retroviral

Expression

Mammalian

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pE-35S-miniSdd7-nCas9-PBE-GmPPO2

Plasmid

#204855

Purpose

For cytosine base editing using miniSdd7 at PPO2 site in soybean (Agrobacterium-mediated transformation)

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd7-nSpCas9(D10A)-UGI-mScarlet

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Promoter

35S, AtU6-26p

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pTK_Sox9_enh_366_Citrine

Plasmid

#130593

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox9_enh_366

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag p38 gamma

Plasmid

#20353

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 gamma (Mapk12 Rat)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_BFP_2A-mIL10

Plasmid

#247507

Purpose

Expresses mouse IL-10 and BFP

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

mouse IL-10 and BFP constitutive (Il10 Mouse, Synthetic)

Use

Lentiviral

Tags

bfp

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pXR004: CasRx pre-gRNA cloning backbone

Plasmid

#109054

Purpose

hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR223-BZLF1(Zta)

Plasmid

#195787

Purpose

Gateway Entry Clone of Epstein-Barr virus BZLF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BZLF1 (BZLF1 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

Plasmid

#223175

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

Insert

hU6_Rfxcas13d-DR1_BsmBI

Use

Lentiviral

Mutation

DR1 A>T

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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CBE-T1.46

Plasmid

#193284

Purpose

Expression of CBE-T1.46 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.46

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-mScarlet

Plasmid

#183461

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-mScarlet fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

mScarlet

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCM121

Plasmid

#220833

Purpose

EryA sensor with increased sensitivity (mphA and mphR (T17R) mutation) and high level resistance (ermC)

Depositor

Matthew Bennett

Article

Miller et al ACS Synth Biol. 2021 Feb 19;10(2):258-26

Insert

mphR

Mutation

T17R

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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CTCF-C CRISPR

Plasmid

#140647

Purpose

CTCF tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CTCF (CTCF Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV2-Smyd2

Plasmid

#13521

Depositor

Shelley Berger

Article

Huang et al Nature. 2006 Nov 30. 444(7119):629-32.

Insert

Smyd2 (Smyd2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 1, 2007

Availability

Academic Institutions and Nonprofits only
Rag2 T490A

Plasmid

#115650

Purpose

expression of full length Rag2 with T490A mutation

Depositor

Martin Gellert

Article

Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

Insert

Rag2 (Rag2 Mouse)

Tags

8XHis-MBP-PreScission

Expression

Mammalian

Mutation

T490A

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1_puro_shNRF2-2

Plasmid

#230087

Purpose

shRNA silencing human NRF2 gene

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15043 - pAAV-AiE1419m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN5043)

Plasmid

#224189

Purpose

AiE1419m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+/Pthlh+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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