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169,450 results

Showing: 11221 - 11250 of 169450 results

  3. pSH-Csy4-T2A-SpRFN

    Plasmid
    #85754
    Purpose
    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Insert
    Csy4-T2A-FokI-dCas9
    Use
    CRISPR
    Tags
    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available Since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85754-plasmid-map-sequence-id-170272

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  4. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

    Plasmid
    #107581
    Purpose
    B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    mCherry
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    pdh promoter Bacillus megaterium DSM319
    Available Since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    107581-plasmid-map-sequence-id-209155

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  6. pAAV-Ef1a-fDIO EYFP (AAV2)

    Viral Prep
    #55641-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a
    Tags
    EYFP (Flp-dependent)
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  7. pSNAPf-C1

    Plasmid
    #58186
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    SNAP tag
    Expression
    Mammalian
    Available Since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    58186-plasmid-map-sequence-id-314094

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  19. pc DNA FRT TO- APPSwed/Ind

    Plasmid
    #114194
    Purpose
    Creation of a stable cell line expressing APP mutation (AD in vitro study)
    Depositor
    Aleksandra Radenovic
    Insert
    Amyloid-Precursor-Protein (APP Human)
    Expression
    Mammalian
    Mutation
    Popular mouse model, overexpress 2 mutations: Swe…
    Promoter
    CMV
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114194-plasmid-map-sequence-id-224256

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  20. pJS167

    Plasmid
    #48881
    Purpose
    Positive feedback plasmid from oscillator
    Depositor
    Jeff Hasty
    Article
    Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi
    Inserts
    araC gene with ssrA tag
    monomeric yeast-enhanced green fluorescent protein
    Use
    Synthetic Biology
    Tags
    ssrA (TSAANDENYALAA)
    Expression
    Bacterial
    Promoter
    Para/lac
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    48881-plasmid-map-sequence-id-71862

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  27. GIPR-DuET

    Plasmid
    #213252
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GIPR (GIPR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213252-plasmid-map-sequence-id-430802

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  30. pDB5318

    Plasmid
    #204828
    Purpose
    An integrating plasmid containing both the inducible enotetS promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.
    Depositor
    Li-Lin Du
    Article
    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404
    Inserts
    tetR
    mECitrine
    Expression
    Yeast
    Promoter
    CMV promoter and enotetS promoter
    Available Since
    Aug. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    204828-plasmid-map-sequence-id-405721

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Showing: 11221 - 11250 of 169450 results