213,568 results

1. Lenti-CMV-MLH1dn-IRES-puroR

   Plasmid

   #207354

   Purpose

   Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   MutL homolog 1 (MLH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Δ754-756 dominant negative mutant

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-Tet-On shYAP1-9

   Plasmid

   #193668

   Purpose

   Tet inducible knockdown of YAP1

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_ATF4_WT

   Plasmid

   #82190

   Purpose

   Gateway Donor vector containing ATF4 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   ATF4 (ATF4 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

   Plasmid

   #60230

   Purpose

   Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   EGFP
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA-P2A and EGFP-KASH

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hSyn

   Available since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. Rich-pT7

   Plasmid

   #108445

   Purpose

   This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pUC57. Xenopus globin 3UTR and 5UTR, pT7, and polyA added.

   Depositor

   Richard Bélanger

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   pT7

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSGEMKv10.1

   Plasmid

   #85704

   Purpose

   Xenopus laevis oocyte expression of Kv10.1

   Depositor

   Luis Pardo

   Article

   Jenke et al EMBO J. 2003 Feb 3;22(3):395-403.

   Insert

   KCNH1 (KCNH1 Human)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFRT-TODestFLAGHAhTTP

   Plasmid

   #48738

   Purpose

   Expresses hTTP

   Depositor

   Thomas Tuschl

   Insert

   TTP (ZFP36 Human)
   

   Use

   Tags

   FLAG, HA, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   June 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pgex-cIAP1

   Plasmid

   #8328

   Depositor

   Jon Ashwell

   Article

   Li et al Nature 2002 Mar 21;416(6878):345-7.

   Insert

   cIAP1 (BIRC2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

9. DDX5

   Plasmid

   #155523

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   DDX5 (DDX5 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. NRIP1_pCSdest

    Plasmid

    #53805

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    NRIP1 (NRIP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pDONR223_GOPC_WT

    Plasmid

    #82924

    Purpose

    Gateway Donor vector containing GOPC, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, Matthew Meyerson

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    GOPC (GOPC Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. rAAV-syn::FLEX-rev::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

    Plasmid

    #32475

    Purpose

    Cre-dependent expression of PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

    Insert

    PSAMQ79G,Q139G-5HT3HC
    

    Use

    AAV and Cre/Lox; Adeno-associated virus, flex swi…

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    Synapsin

    Available since

    Dec. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_SLC25A3

    Plasmid

    #132040

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A3 (SLC25A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pIVT

    Plasmid

    #122139

    Purpose

    For making cRNA appropriate for microinjection

    Depositor

    Carmen Williams

    Article

    Igarashi et al Dev Biol. 2007 Dec 1;312(1):321-30. Epub

    Type

    Empty backbone

    Use

    For in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET24a-LaM4-EGFP

    Plasmid

    #182641

    Purpose

    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    anti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, EGFP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#45

    Plasmid

    #153526

    Purpose

    Expression of the anti SARS-CoV-2_RBD sybody Sb#45

    Depositor

    Markus Seeger

    Article

    Walter et al bioRxiv 2020.04.16.045419

    Insert

    Sb#45
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV x EF1a_EKAREN5(TA)-ires-puro

    Plasmid

    #167823

    Purpose

    control sensor for EKAREN5

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5(TA)
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAV-U6+27-Tornado-Pepper-RhoBAST

    Plasmid

    #196343

    Purpose

    Overexpression of circular Pepper-RhoBAST

    Depositor

    Murat Sunbul

    Article

    Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

    Insert

    Tornado-Pepper-RhoBAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. IRE1b.WT.9E10.pCDNA3

    Plasmid

    #21880

    Depositor

    David Ron

    Insert

    IRE1b (Ern2 Mouse)
    

    Use

    Tags

    Myc (9E10)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. LVXN-Neo-NSD2-E1099K

    Plasmid

    #86011

    Purpose

    express NSD2 E1099K mutant in mammalian cells

    Depositor

    Darrin Stuart

    Article

    Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

    Insert

    NSD2 E1099K mutant (NSD2 Human)
    

    Use

    Tags

    Flag tag  D Y K D D D D K

    Expression

    Mammalian

    Mutation

    E1099K; K1002R (please see depositor comments bel…

    Promoter

    CMV

    Available since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only