Skip to main content
Addgene
Showing: 1901 - 1950 of 2660 results

  1. p-dCas9-LSD1-Hygro

    Plasmid
    #104406
    Purpose
    transient expression of dCas9-LSD1 fusion protein
    Depositor
    Stephan Beck
    Insert
    LSD1 (KDM1A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    IRATp970A0364D (Source Bioscience) has a P405H ch…
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104406-plasmid-map-sequence-id-204465

    Enlarge

  7. p-dCas9-HDAC1-H141A-Hygro

    Plasmid
    #104417
    Purpose
    transient expression of dCas9-dHDAC1 fusion protein
    Depositor
    Stephan Beck
    Insert
    dHDAC1 (HDAC1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    H141A
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104417-plasmid-map-sequence-id-204223

    Enlarge

  8. HA-Clover-HIF-2alpha T406E

    Plasmid
    #163379
    Purpose
    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Thr406Glu) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    T406E
    Promoter
    CMV Promoter
    Available since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    163379-plasmid-map-sequence-id-322519

    Enlarge

  9. HA-Clover-HIF-2alpha T406A

    Plasmid
    #163378
    Purpose
    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Thr406Ala) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    T406A
    Promoter
    CMV Promoter
    Available since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    163378-plasmid-map-sequence-id-322518

    Enlarge

  10. HA-Clover-HIF-2alpha S581D

    Plasmid
    #163377
    Purpose
    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Ser581Asp) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    S581D
    Promoter
    CMV Promoter
    Available since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    163377-plasmid-map-sequence-id-322517

    Enlarge

  11. HA-Clover-HIF-2alpha S581A

    Plasmid
    #163376
    Purpose
    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Ser581Ala) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    S581A
    Promoter
    CMV Promoter
    Available since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    163376-plasmid-map-sequence-id-322516

    Enlarge

  12. HA-Clover-HIF-2alpha T528E

    Plasmid
    #163375
    Purpose
    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Thr528Glu) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    T528E
    Promoter
    CMV Promoter
    Available since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    163375-plasmid-map-sequence-id-322515

    Enlarge

  13. HA-Clover-HIF-2alpha T528A

    Plasmid
    #163374
    Purpose
    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Thr528Ala) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    T528A
    Promoter
    CMV Promoter
    Available since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    163374-plasmid-map-sequence-id-322511

    Enlarge

  14. HA-Clover-HIF-2alpha S345D

    Plasmid
    #163373
    Purpose
    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Ser345Asp) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    S345D
    Promoter
    CMV Promoter
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    163373-plasmid-map-sequence-id-322298

    Enlarge

  15. HA-Clover-HIF-2alpha S345A

    Plasmid
    #163372
    Purpose
    Express HA- and Clover-tagged HIF-2alpha (with a site-directed mutation Ser345Ala) plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 2 alpha (EPAS1 Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    S345A
    Promoter
    CMV Promoter
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    163372-plasmid-map-sequence-id-322296

    Enlarge

  16. psglessClonePX

    Plasmid
    #87193
    Purpose
    used for downstream cloning, contains CMV promoter with NheI and XhoI cloning sites flanking a partial insert that gets excised when adding an insert of choice
    Depositor
    Thomas Cech
    Article
    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87193-plasmid-map-sequence-id-170165

    Enlarge

  17. pDN-D2irTNG4kwh

    Plasmid
    #44722
    Depositor
    Gabor Balazsi
    Article
    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1
    Inserts
    pCMV-D2i promoter
    htetR::NLS::EGFP
    Use
    Synthetic Biology; Expression regulator/reporter;…
    Tags
    Rabbit β-globin intron II and WPRE
    Expression
    Mammalian
    Mutation
    Initiator motif (Inr) displaced relative to pCMV-…
    Promoter
    pCMV-D2i
    Available since
    April 23, 2013
    Availability
    Academic Institutions and Nonprofits only
    44722-plasmid-map-sequence-id-61263

    Enlarge

  18. pRRLsin-SV40 T antigen-IRES-mCherry

    Plasmid
    #58993
    Purpose
    lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter
    Depositor
    Snorri Thorgeirsson
    Article
    Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31
    Inserts
    CMV enhancer-chicken beta actin promoter
    SV40 large T antigen
    IRES-mCherry red fluorescent protein
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    EcoRV site in MCS converted to BamHI site
    Promoter
    CMV enhancer-chicken beta actin
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    58993-plasmid-map-sequence-id-457463

    Enlarge

  19. pLJM1-FIRE-pHLy

    Plasmid
    #170775
    Purpose
    Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells
    Depositor
    Aimee Kao
    Article
    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse
    Insert
    LAMP1 (LAMP1 Human)
    Use
    Lentiviral
    Tags
    mCherry and mTFP1
    Expression
    Mammalian
    Mutation
    84 nucleotide Signal Sequence switched to the beg…
    Promoter
    CMV
    Available since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170775-plasmid-map-sequence-id-339023

    Enlarge

  21. EBFP2-Cx26

    Plasmid
    #69021
    Purpose
    Expresses rat Cx26 (Gjb2 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Gja2 (Gjb2, Cx26 Rat)
    Use
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    69021-plasmid-map-sequence-id-127473

    Enlarge

  27. pCAG_iPE-C

    Plasmid
    #214735
    Purpose
    encodes iPE-C prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the C terminus of nCas9-N863A) driven by a CAG promoter
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4
    Insert
    CAG_iPE-C_bGH
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214735-plasmid-map-sequence-id-425000

    Enlarge

  28. pEGFP-NMHC-IIA-S1916A

    Plasmid
    #101040
    Purpose
    Expresses GFP-MYH9 construct (human myosin IIA) carrying mutation of serine 1916 to alanine, driven by CMV promoter. Thus inhibits PKC phosphorylation of the tail. Also carries S1915A mutation.
    Depositor
    Tom Egelhoff
    Article
    Rai et al J Biol Chem. 2017 Feb 24;292(8):3099-311
    Insert
    Non-muscle myosin IIA heavy chain (MYH9 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    S1916A, S1917A
    Promoter
    CMV
    Available since
    Nov. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    101040-plasmid-map-sequence-id-198956

    Enlarge

  29. pCAG_iPE-N

    Plasmid
    #214734
    Purpose
    encodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoter
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4
    Insert
    CAG_iPE-N_bGH
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214734-plasmid-map-sequence-id-453897

    Enlarge

  30. pLenti4/TO-hEPO

    Plasmid
    #50437
    Purpose
    express human erythropoietin under the control of tetracycline-regulatable CMV promoter using lentiviral delivery system
    Depositor
    Juan Melero-Martin
    Article
    Lin et al Blood. 2011 Nov 17;118(20):5420-8. doi:
    Insert
    EPO (EPO Human)
    Use
    Lentiviral; Tetracycline-regulatable (t-rex)
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/TO
    Available since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  32. Human a3 nicotinic receptor subunit EM construct

    Plasmid
    #163960
    Purpose
    pEZT-BM vector with alpha3 gene after CMV promoter, published EM construct
    Depositor
    Ryan Hibbs
    Article
    Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do
    Insert
    Human alpha3 nicotinic acetylcholine receptor subunit EM construct
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    apocytochrome b(562)RIL (BRIL)
    Expression
    Mammalian
    Mutation
    Asn348-Ser402 replaced with apocytochrome b(562)R…
    Promoter
    CMV
    Available since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163960-plasmid-map-sequence-id-323882

    Enlarge

  33. ATG-1929

    Plasmid
    #108712
    Purpose
    CBR2opt in pF4Ag expression vector with T7 and CMV promoter
    Depositor
    Keith Wood
    Article
    Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10
    Insert
    CBR2opt
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promega used directed evolution to create CBR (Cl…
    Promoter
    T7, CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108712-plasmid-map-sequence-id-224541

    Enlarge

  36. pEGFP-NMHC-IIA-3xA

    Plasmid
    #101041
    Purpose
    Expresses GFP-MYH9 construct (human myosin IIA) carrying S1943A mutation and S1916A, S1915A mutation, driven by CMV promoter. Thus inhibits CKII and PKC phosphorylation of the tail.
    Depositor
    Tom Egelhoff
    Article
    Rai et al J Biol Chem. 2017 Feb 24;292(8):3099-311
    Insert
    Non-muscle myosin IIA heavy chain (MYH9 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    S1915A, S1916A, S1943A
    Promoter
    CMV
    Available since
    Nov. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    101041-plasmid-map-sequence-id-343684

    Enlarge

  37. Human b4 nicotinic receptor subunit EM construct

    Plasmid
    #163961
    Purpose
    pEZT-BM vector with beta4 gene after CMV promoter, published EM construct
    Depositor
    Ryan Hibbs
    Article
    Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do
    Insert
    Human beta4 nicotinic acetylcholine receptor subunit EM construct
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    StrepII tag and apocytochrome b(562)RIL (BRIL)
    Expression
    Mammalian
    Mutation
    Pro341-Ser395 replaced with apocytochrome b(562)R…
    Promoter
    CMV
    Available since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163961-plasmid-map-sequence-id-323883

    Enlarge

  44. pSBL_DK202

    Plasmid
    #156426
    Purpose
    Mammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells
    Depositor
    Stephen Brohawn
    Article
    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.
    Insert
    SARS-CoV-2 3a protein (aa 42-275)
    Use
    Tags
    EGFP-His
    Expression
    Mammalian
    Mutation
    aa 42-275
    Promoter
    CMV
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156426-plasmid-map-sequence-id-306303

    Enlarge

Showing: 1901 - 1950 of 2660 results