We narrowed to 22,843 results for: TES

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prk5-TMEM192-3xHA PM mutant

Plasmid

#149506

Purpose

Transient expression of human TMEM192. Dileucine motifs LL23-24 and LL28-29 have been mutated to AA.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

TMEM192 (TMEM192 Human)

Use

Transient expression

Tags

3XHA

Expression

Mammalian

Mutation

p.LL23-24AA, p.LL28-29AA

Promoter

CMV

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+36)-DL4

Plasmid

#199168

Purpose

used to express a supercharged GFP variant that forms inclusion bodies in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+36)-DL4

Tags

MGHHHHHHGGA

Mutation

E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

Promoter

T7

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4-BG505.DS-SOSIP.6mut-gp160

Plasmid

#123278

Purpose

Mammalian expression plasmid for Env from the BG505 HIV-1 isolate; DS-SOSIP.6mut variant

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BG505) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; SOSIP mutations; …

Promoter

CMV

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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G718A_BattB(GA)_TKterm_AttB(GT)_sGFP-PTEN-IRES-mCherry-P2A-HygroR_noTerm_attP(GA)_IRES-UnaG-2A-Puro-Term

Plasmid

#200634

Purpose

Plasmid self-excising recombination plasmid for Matreyek Bxb1(GT) landing pad

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

PTEN (PTEN Human)

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMC060-HisMS2_PLP_Env_pac

Plasmid

#155040

Purpose

Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Envelope Gene

Depositor

Paul Freemont

Article

Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

Inserts

Maturation Protein
Coat Protein Dimer
Envelope Gene

Expression

Bacterial

Mutation

Remove TypeIIs Restriction Sites

Promoter

T7

Available Since

Sept. 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET-Duet1-6xHis-TEV-LC3BGlydelta5C

Plasmid

#190237

Purpose

Plasmid for the expression and purification of 6xHis-TEV-LC3BGlydelta5C. Internal reference: SMC893.

Depositor

Sascha Martens

Article

Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

MAP1LC3B (MAP1LC3B Human)

Tags

6xHis-TEV

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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myc-Atg14 S29A

Plasmid

#86756

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

atg14 (ATG14 Human)

Tags

myc

Expression

Mammalian

Mutation

S29A

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_BH3-4E

Plasmid

#187144

Purpose

mammalian cell expression of FLAG-HUWE1 V1976E/V1780E/L1983E/M1987E

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

V1976E/V1780E/L1983E/M1987E

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_delta3291-3298

Plasmid

#187125

Purpose

mammalian cell expression of FLAG-HUWE1 delta3291-3298

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

delta3291-3298

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Muc5b D1 (murine) with cleavable His tag

Plasmid

#181814

Purpose

Expresses murine Muc5b D1 with cleavable His tag, residues 50-426

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

Muc5b D1 (Muc5b Mouse)

Tags

TEV cleavage site followed by 6xHistidine tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(703-800)-Strep

Plasmid

#141344

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

Insert

GB1-His6-TEV-ALIX(703-800)-Strep

Tags

GB1-His6-TEV and Strep-tag (WSHPQFEK)

Expression

Bacterial

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pX458-GFP-TRIM28

Plasmid

#185010

Purpose

Encodes gRNA for mouse TRIM28 along with Cas9 with 2A-EGFP

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

TRIM28 sgRNA (Trim28 Mouse)

Use

CRISPR

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-hSTING-dCTT

Plasmid

#214164

Purpose

Retroviral transduction of STING

Depositor

Nan Yan

Article

Wu et al J Exp Med. 2019 Apr 1;216(4):867-883. do

Insert

STING (dCTT) (STING1 Human)

Use

Retroviral

Mutation

dCTT

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot4-GB1His6_AG

Plasmid

#148792

Purpose

Bacterial Expression of HsNot4

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4 (CNOT4 Human)

Expression

Bacterial

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP5.v2 puro

Plasmid

#87793

Purpose

Expresses an inducible short hairpin targeting human MKP5 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP5 (DUSP10 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP5.v1 puro

Plasmid

#87792

Purpose

Expresses an inducible short hairpin targeting human MKP5 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP5 (DUSP10 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3_shRNA resistant PKA-Cα-mEGFP

Plasmid

#114143

Purpose

Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown

Expression

Mammalian

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR221-CCL5.Pro

Plasmid

#79492

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CCL5 Promoter (CCL5 Human, Synthetic)

Use

Multisite gateway entry clone

Promoter

CCL5 Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRVdG-4GCaMP6f

Plasmid

#98035

Purpose

First-generation rabies viral vector genome

Depositor

Ian Wickersham

Article

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

GCaMP6f

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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B1 Integrin

Plasmid

#186137

Purpose

For expression of recombinant beta1 integrin cytosolic domain in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

ITGB1 (ITGB1 Human)

Tags

MBP and untranslated His6 after stop codon

Expression

Bacterial

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pColdI-GST-mGTSF1l

Plasmid

#214817

Purpose

Bacterial expression of GST-tagged mouse GTSF1l

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

GTSF1l (Gtsf1l Mouse)

Tags

6x His and GST

Expression

Bacterial

Promoter

CspA

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Braf R188L

Plasmid

#118330

Purpose

Expression of human Braf

Depositor

Philip Stork

Article

Li et al J Biol Chem. 2013 Sep 20;288(38):27646-5

Insert

Braf R188L (BRAF Human)

Tags

GFP

Expression

Mammalian

Mutation

R188L

Promoter

CMV

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast

Plasmid

#166413

Purpose

express hPOT1 ΔOB in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

Protection Of Telomeres 1 (POT1 Human)

Use

Lentiviral

Tags

myc

Expression

Mammalian

Mutation

ΔOB deleted 125 a.a

Promoter

ef1-a

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGL4.24-Cd36-enhancer8

Plasmid

#138580

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17923535_17924934

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Promoter

Minimal promoter

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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AdMKP-3 S197A

Plasmid

#131522

Purpose

Expresses MKP-3 with S197A mutation in mammalian cells

Depositor

Haiyan Xu

Article

Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

Insert

Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)

Use

Adenoviral

Tags

No

Mutation

changed Serine 197 to Alanine

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Cx43-Myc

Plasmid

#60693

Purpose

mammalian expression of Myc-tagged rat Cx43

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
tet-pLKO shMKP1.v2 puro

Plasmid

#87791

Purpose

Expresses an inducible short hairpin targeting human MKP1 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP1 (DUSP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Bmi1

Plasmid

#61532

Purpose

Expresses Bmi1 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Bmi1 (Bmi1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
7432 Bicistronic_GFP_ires_puro

Plasmid

#64336

Purpose

This a retroviral expression plasmid expressing GFP along with puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

GFP

Use

Retroviral

Tags

GFP

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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2xNLS-NLP-cMyc LbaCas12a

Plasmid

#182123

Purpose

pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

2xNLS-NLP-cMyc LbaCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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