We narrowed to 13,810 results for: cas9 genes

1. pLV.U6.gLacZ.Cas9-T2A-GFP

   Plasmid

   #170361

   Purpose

   Negative control plasmid used for Crispr/cas9 based disruption.

   Depositor

   Johan Jakobsson

   Article

   Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

   Insert

   Cas9-T2A-GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBbdCas9S(RBSmut)_Psyn-sgRNAmreB

   Plasmid

   #149594

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi mreB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAmreB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SIN-PGK-Cas9-WPRE

   Plasmid

   #87886

   Purpose

   To produce Lentiviral vector for gene editing

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   hCas9
   

   Use

   Lentiviral

   Tags

   nls

   Expression

   Mutation

   Human codon-optimized

   Promoter

   mouse PGK

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pD2-dcas9-J23101-GFP

   Plasmid

   #122260

   Purpose

   Constitutive dCas9 and GFP expression plasmid

   Depositor

   Chueh Loo Poh

   Article

   Zhang et al Sci Rep. 2018 Sep 3;8(1):13127. doi: 10.

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBbdCas9S(RBSmut)_Psyn-sgRNArodA

   Plasmid

   #149656

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi rodA

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNArodA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTR221-H1-sgGFP1-7SK-sgCas9

   Plasmid

   #87908

   Purpose

   multiple sgRNA

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   sgGFP1, sgCas9
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   H1, 7SK

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2

   Plasmid

   #141052

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   eSpCas9(1.1)-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMK33/intein-Cas9_S219-3XFLAG

   Plasmid

   #133782

   Purpose

   Expresses human codon-optimized intein-Cas9

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

   Insert

   Human codon-optimized intein-Cas9
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-SpCas9HF1-P2A-puro

   Plasmid

   #214357

   Purpose

   Expresses SpCas9HF1 and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SpCas9HF1 and puromycin resistance genes
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-SlugCas9-P2A-puro

    Plasmid

    #214353

    Purpose

    Expresses SlugCas9 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCas9 and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMB1-A-ptet-FLAG-dCas9

    Plasmid

    #190130

    Purpose

    Level 1 MoClo plasmid containing an aTC inducible FLAG tagged dCas9 for CRISPRi

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCBH-Cas9-Suntag-BB_BbsI

    Plasmid

    #164805

    Purpose

    REDIT backbone for SunTag Cas9, pU6-gRNA-backbone(BbsI)-CBH-SpCas9-Suntag-T2A-EBFP

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBbdCas9S(-10AC1)_Psyn-sgRNArodA

    Plasmid

    #149659

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10AC1), Psyn

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBbdCas9S(-10AC2)_Psyn-sgRNArodA

    Plasmid

    #149660

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10AC2), Psyn

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBbdCas9S(-10TC)_Psyn-sgRNAmreB

    Plasmid

    #149595

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBbdCas9S(-10TC)_Psyn-sgRNAftsI

    Plasmid

    #149591

    Purpose

    all-in-all CRISPRi vector for targeting B. burgdorferi ftsI

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAftsI
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBbdCas9S(-10TC)_Psyn-sgRNArodA

    Plasmid

    #149657

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBbdCas9S(-10TC)_Psyn-sgRNAflaB

    Plasmid

    #149589

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi flaB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAflaB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10TC), Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR-L3-Csy4-Cas9-EGFP-L2

    Plasmid

    #141045

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Csy4-2A-Cas9-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR-L3-eSpCas9(1.1)-2A-EGFP-L2

    Plasmid

    #141051

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    eSpCas9(1.1)-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pENTR-L3-Csy4-FokI-dCas9-L2

    Plasmid

    #141042

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Csy4-2A-FokI-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pENTR-L3-SpCas9-HF4-2A-EGFP-L2

    Plasmid

    #141049

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    SpCas9-HF4-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pENTR-L3-SpCas9-HF2-2A-EGFP-L2

    Plasmid

    #141050

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    SpCas9-HF2-2A-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFGRNA-LbCpf1-spCas9-MS2

    Plasmid

    #119675

    Purpose

    Empty vector for cloning LbCpf1-spCas9 MS2-fgRNA

    Depositor

    Yongsub Kim

    Article

    Kweon et al Nat Commun. 2018 Jan 16;9(1):303. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Lenti-dCas9-KRAB-blast

    Plasmid

    #89567

    Purpose

    Plasmid expression dCas9 protein in fusion with KRAB domain

    Depositor

    Gary Hon

    Article

    Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    Blasticidin resistance gene and KRAB

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pB-CAGGS-dCas9-KRAB-MeCP2

    Plasmid

    #110824

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MiniCoopR 2xU6:gRNA, mitfa:Cas9

    Plasmid

    #118844

    Purpose

    Targets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Type

    Empty backbone

    Use

    CRISPR; Tol2

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. eSpCas9(1.1)-T2A-Puro

    Plasmid

    #101039

    Purpose

    Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.

    Depositor

    Andrea Németh

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3xFLAG and T2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. px551-mSncg-0.27kb-HA-SpCas9

    Plasmid

    #153208

    Purpose

    Truncated Mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates HA and Cas9 expression in retinal ganglion cells

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Insert

    Cas9
    

    Use

    AAV and CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated mSncg (0.27kb)

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHDE-35S-Cas9-mCherry

    Plasmid

    #78931

    Purpose

    For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants

    Depositor

    Yunde Zhao

    Article

    Gao et al Plant Physiol. 2016 May 15. pii: pp.0066

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. EFS-SpdCas9-Dnmt3A/3L-catΔ

    Plasmid

    #222501

    Purpose

    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) followed by P2A-mCherry

    Depositor

    Isaac Hilton

    Article

    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

    Insert

    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
    

    Use

    Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; C706A and R832…

    Promoter

    EFS

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET-Octo_VirD2_NLS-Cas9-6xHis

    Plasmid

    #232296

    Purpose

    Expresses Cas9 protein fused with octopine-type VirD2-originating NLS

    Depositor

    Hee-Sik Kim

    Article

    Le et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

    Insert

    Octo_VirD2_NLS-Cas9-6xHis
    

    Use

    CRISPR

    Tags

    6xHis and Octopine-type VirD2-derived NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D

    Plasmid

    #169912

    Purpose

    Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    dCjCas9
    

    Use

    CRISPR

    Tags

    10XGCN4, 2xSV40NLS, and SV40NLS-HA

    Expression

    Mammalian

    Mutation

    D8A, H559A in cjCas9

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLenti-eGFP-dSaCas9-blast

    Plasmid

    #191653

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    eGFP-T2A-dSaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 1137=Hsp70Bb-Cas9[dsRed]

    Plasmid

    #149424

    Purpose

    The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Dmel Hsp70Bb

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GCN5 FL-dCas9-CBP core

    Plasmid

    #179556

    Purpose

    encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. SIN-PGK-Cas9-V5-WPRE

    Plasmid

    #87876

    Purpose

    To produce lentiviral vector for Cas9 editing

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    spCas9
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Human codon-optimized

    Promoter

    mouse PGK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-CMV-Dpi2Cas9-puro

    Plasmid

    #232113

    Purpose

    Expresses Dpi2Cas9 and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-CMV-Slu1Cas9-puro

    Plasmid

    #232111

    Purpose

    Expresses Slu1Cas9 and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-CMV-Slu2Cas9-puro

    Plasmid

    #232112

    Purpose

    Expresses Slu2Cas9 and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge