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pKP113 (pRS406-PRC1p-PRC1-GFP-ADH1t)

Plasmid

#106464

Purpose

Expresses Prc1 with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

PRC1

Tags

GFP

Expression

Bacterial and Yeast

Promoter

PRC1

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVII-BFP

Plasmid

#162755

Purpose

Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

mTagBFP2

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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PCS2+Cas9-mSA

Plasmid

#103882

Purpose

Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

CAS9-MSA

Use

CRISPR

Tags

MSA (monomeric streptavidin

Mutation

none

Promoter

SP6

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLARRYv2-EGFP

Plasmid

#233208

Purpose

Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP marker

Depositor

Alejo Rodriguez-Fraticelli

Article

Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pYPet-His

Plasmid

#14031

Depositor

Patrick Daugherty

Article

Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

Insert

YPet

Tags

6xHis

Expression

Bacterial

Available Since

Feb. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

Plasmid

#162378

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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plex_307-ccdB-hygro

Plasmid

#201143

Purpose

pLEX_307 plasmid with hygro marker

Depositor

Alejandro Chavez

Type

Empty backbone

Expression

Mammalian

Promoter

EF-1α

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C

Plasmid

#154197

Purpose

Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

sgRNA-BC2-A, sgRNA-BC2-C

Use

Lentiviral; Catch barcode activation

Expression

Mammalian

Promoter

hU6 and mU6

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pJL179

Plasmid

#243688

Purpose

Stably expresses SUPV3L1 CDS, flanked with endogenous 5' and 3'UTRs of ALDH18A1

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

UTRs and CDS of SUPV3L1 (SUPV3L1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pWZ854

Plasmid

#208116

Purpose

mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-Neo

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Insert

mNeonGreen-HPRT1-Neo

Tags

T2A

Expression

Bacterial and Mammalian

Promoter

EF1alpha

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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mOrange-N1

Plasmid

#54499

Purpose

Localization: N1 Cloning Vector, Excitation: 548, Emission: 562

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Tags

mOrange

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pEHAC1-HA-BirA-GSGS-Traf6

Plasmid

#232586

Purpose

Express BirA tagged to the N-terminus of TRAF6 with a GSGS linker between BirA and TRAF6.

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

BirA -TRAF6 (TRAF6 Human)

Tags

HA

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mScarlet-I

Plasmid

#159172

Purpose

To express a bright monomeric red FP for labelling and detection of mammalian cells

Depositor

Pantelis Tsoulfas

Insert

mScarlet-I

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Mutation

Codons were optimized to Human codon usage

Promoter

CMV

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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Shank3-HA/pRK5

Plasmid

#197335

Purpose

Plasmid expressing an antigen targeting Shank3

Depositor

Morgan Sheng

Insert

Shank3 (Shank3 Rat)

Tags

HA

Expression

Mammalian

Available Since

June 16, 2023

Availability

Academic Institutions and Nonprofits only

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VAMP2-pHmScarlet

Plasmid

#166890

Purpose

For vesicle exocytosis visualization using pHmScarlet

Depositor

Pingyong Xu

Article

Liu et al Nat Commun. 2021 Mar 3;12(1):1413. doi:

Insert

VAMP2 (Vamp2 Mouse)

Tags

pHmScarlet

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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AiP5003 pAAV-Syn-DIO-TVA66T-dTom-CVS-N2cG

Plasmid

#176285

Purpose

Cre-dependent AAV virus

Depositor

Allen Institute for Brain Science, Shenqin Yao

Article

Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

Insert

TVA66T-dTomato-rabies Glycoprotein

Tags

dTomato

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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PKMYT1 gRNA (BRDN0001146750)

Plasmid

#77280

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKMYT1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKMYT1 (PKMYT1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HRV-3C Protease-mCherry

Plasmid

#243792

Purpose

Encodes for mammalian expression of HRV-3C protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

HRV-3C protease

Expression

Mammalian

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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4xCSL-luciferase

Plasmid

#41726

Depositor

Raphael Kopan

Article

Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

Insert

4xCSL binding sites

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Contains a multimerized high affinity CSL binding…

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pEF1a-Ronin-FLAG-IRES-Neo

Plasmid

#28020

Depositor

Thomas Zwaka

Article

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Insert

Ronin (THAP11 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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