We narrowed to 14,950 results for: RING

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4LNDDE488Q-pCDNA3.1(+)

Plasmid

#172228

Purpose

Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) in mammalian cells

Depositor

Joshua Rosenthal

Article

Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

Insert

Lambda N peptide - human ADAR2 Deaminase Domain E488Q

Tags

6xHis and Flag

Expression

Mammalian

Mutation

changed the Glutamic acid 488 in the Deaminase Do…

Promoter

CMV

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAG668 lifeAct-pFAST

Plasmid

#172866

Purpose

Expresses LifeAct-pFAST in mammalian cells (actin labeling)

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

LifeAct-pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCVL.Active/Repressed TLR (Sce)

Plasmid

#45575

Purpose

Codes for the Active/Repressed TLR with the target site for I-Sce I nuclease

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

eGFP with I-Sce I TS
+3 mCherry

Use

Lentiviral

Tags

T2A dislinker and iRFP

Expression

Bacterial and Mammalian

Mutation

embedded I-Sce I TS from 163-185

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pCAD16_CAD_12-490_pvp008

Plasmid

#124842

Purpose

Expresses Aspergillus terreus CAD in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-11, synthetic codon optimiz…

Promoter

T7

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG MCP-VP64

Plasmid

#92357

Purpose

CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

MCP-VP64

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJZC638

Plasmid

#62283

Purpose

Expresses MCP-VP64 and dCas9 in Yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

MCP-VP64
dCas9

Tags

VP64

Expression

Yeast

Mutation

Nuclease activity has been deactivated by mutatio…

Promoter

pAdh and pGal10

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pMOD_C2518

Plasmid

#91087

Purpose

Module C, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

TaU6

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pFNC-3

Plasmid

#233296

Purpose

Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.

Depositor

Pablo Ivan Nikel

Article

Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

Inserts

BxbI phage integrase
sacB

Expression

Bacterial

Promoter

PEM7 and unknown

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pJPT018-LAT_WT-HaloTag-3x FLAG

Plasmid

#202502

Purpose

Express WT-LAT

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

LAT (LAT Human)

Tags

HaloTag-3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pBLO ddd64.1

Plasmid

#123121

Purpose

E. coli expression vector for catalytically inactivated deltaproteobacteria CasX

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

CasX

Use

CRISPR

Mutation

Mutated amino acids in catalytic triad to alanine…

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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px459-Hira KO sgRNA

Plasmid

#186938

Purpose

Hira KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Hira KO sgRNA (Hira Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDF0430 huDisCas7-11-S1006-GGGS-D1221-U6-pro-Gluc

Plasmid

#186987

Purpose

AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of Gluc crRNA guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-s1006-gggs-d122, Gluc crRNA guide

Use

AAV

Mutation

s1006-gggs-d1221 deletion

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBTK569

Plasmid

#110614

Purpose

BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

E2-Crimson

Expression

Bacterial

Mutation

V32M (see comments below)

Available Since

Oct. 2, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/TO-LacR-TopBP1

Plasmid

#31313

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

Insert

TopBP1 (TOPBP1 Human)

Tags

FLAG and LacR

Expression

Mammalian

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pBbE1k-3

Plasmid

#71722

Purpose

tyrosine hydroxylase from mouse, dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

Depositor

Taek Soon Lee

Article

Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

Inserts

dihydropterine reductase (QDPR Human)
pterin-4a-carbinolamine dehydratase (PCBD1 Human)

Promoter

pTrc

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BaLgp140-8his

Plasmid

#100924

Purpose

Mammalian expression plasmid for soluble gp140 from the BaL HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Tags

CD5 leader sequence and Purification tag after D6…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pC009 LshCas13a locus with targeting spacer

Plasmid

#91900

Purpose

LshCas13a locus into pACYC184 with targeting spacer

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LshCas13a locus with targeting spacer

Expression

Bacterial

Available Since

Oct. 16, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pTrc99S-ssDsbA-MBP-4xDQNAT

Plasmid

#128398

Purpose

Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Maltose binding protein

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Mutation

Y342H- please see depositor comments

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSR357.24

Plasmid

#125109

Purpose

Expression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-ydfI(DBD)159
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-haA3A-CBE-G-C

Plasmid

#220899

Purpose

AAV genome encoding the C-terminal of haA3A-CBE-G, and sgRNA

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-G-C

Use

AAV

Expression

Mammalian

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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