-
Plasmid#193864PurposeE. coli expression vector for N-ter GFP11 and C-ter R9 tagged mCherry.DepositorInsertmCherry
UseTags6xHis, GFP11, and R9ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
1BR9-mCherrySTOP
Plasmid#193866PurposeE. coli expression vector for N-ter GFP11 tagged mCherry.DepositorInsertmCherry
UseTags6xHis and GFP11ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin 77-465
Plasmid#45970DepositorInsertParkin 77-465 (Prkn Rat)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Dele…PromotertacAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0503
Plasmid#91013PurposeModule A, Promoter: CmYLCV, Gene: Csy4-P2A-AtCas9, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9
UseCRISPRTagsExpressionMutationPromoterCmYLCVAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX208-gLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA
Plasmid#125233PurposeExpresses "sender" trans-cellular NanoLuc in mammalian cellsDepositorInsertgLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pChlr-8CQ
Plasmid#52171PurposeEncodes module 8 with amino acids 12C-16Q for recognition of nt 1ADepositorInsertPumilio homology domain amino acids 284-319 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12C in module 8PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPPC017.109
Plasmid#171145PurposeExpression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmidDepositorInsertsJ109 scRNA
J1-BBa_J23117-mRFP
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J1-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239A (dFnCas12a)-TV
Plasmid#136379PurposeCRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation systemDepositorInsertdFnCas12a-TV
UseCRISPRTagsExpressionPlantMutationD917APromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_RAB5A
Plasmid#111692PurposeMAC-tagged gene expressionDepositorInsertRAB5A (RAB5A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_GART
Plasmid#183296PurposeAll-in-One CRISPRko system with a guide RNA that targets GART geneDepositorInsertGART
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3013
Plasmid#91100PurposeModule C, Promoter: PvUbi2, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedTagsExpressionMutationPromoterPvUbi2Available sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTK_Citrine_Nanotag_5_neg_Control
Plasmid#130558PurposeEnhancer cloning nanotag reporter vectorDepositorInsertnegative oligo sequence
UseEnhancer cloning nanotag reporter vectorTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYC119
Plasmid#63916PurposeRecipient overexpressing vector to construct FLAG tagged fusionsDepositorTypeEmpty backboneUseTagsFLAGExpressionYeastMutationPromoterTEF1 (Saccharomyces cerevisiae)Available sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET20b-GpNLuc-6xHis
Plasmid#135932PurposeExpresses the GpNLuc LumiFluor in bacterial cells.DepositorInsertGpNLuc LumiFluor
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3501
Plasmid#91050PurposeModule A, Promoter: 35S, Gene: dAtCas9-VPR, Terminator: HSPDepositorInsertdAtCas9-VPR
UseCRISPRTagsExpressionMutationPromoter35SAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTV-Nanog Hyg
Plasmid#13836DepositorInsertIRES-Hyg-pA cassette flanked by Nanog homologous regions (Nanog Mouse)
UseBeta-galTagsExpressionMammalianMutationNonePromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFP.R375
Plasmid#138245PurposeExpresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109As-gfp
Plasmid#123143PurposeAn arsenic sensor with optimized intracellular receptor density. pSB3K3 carrying J109-30arsR-t-ParsR-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, ParsRAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEBB-BT-Ubiquitin
Plasmid#36098DepositorInsertUbiquitin (RPS27A Human)
UseTagsTB-tagExpressionMammalianMutationPromoterEF1aAvailable sinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A4110
Plasmid#91056PurposeModule A, Promoter: ZmUbi, Gene: Csy4-P2A-dTaCas9-VPR, Terminator: HSPDepositorInsertCsy4-P2A-dTaCas9-VPR
UseCRISPRTagsExpressionMutationPromoterZmUbiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa3.8
Plasmid#136376PurposeGateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 is usedDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCK300
Plasmid#87766Purposeempty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacIDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMS10: pTns(AvCAST)_ΔTnsD
Plasmid#168143PurposeInducible expression of AvCAST TnsA, TnsB, TnsC and TniQ proteins.DepositorInsertAvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1210
Plasmid#91033PurposeModule A, Promoter: ZmUbi, Gene: TaCas9_D10A nickase, Terminator: HSPDepositorInsertTaCas9_D10A nickase
UseCRISPRTagsExpressionMutationD10APromoterZmUbiAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_FGFR1
Plasmid#183289PurposeAll-in-One CRISPRko system with a guide RNA that targets FGFR1 geneDepositorInsertFGFR1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_FGFR2
Plasmid#183290PurposeAll-in-One CRISPRko system with a guide RNA that targets FGFR2 geneDepositorInsertFGFR2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_25K
Plasmid#91147PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TALEN, Plant Selection: PvUbi2:hpt IIDepositorInsertEngineering Reagent: ZmUbi:TALEN
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_TR61B
Plasmid#111679PurposeMAC-tagged gene expressionDepositorInsertTR61B (TRMT61B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G0-R0
Plasmid#101803PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterSpy 1049Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin W403A
Plasmid#45974DepositorInsertParkin W403A (Prkn Rat)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromotertacAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTK_Citrine_Nanotag_24_positive_Control
Plasmid#130570PurposeEnhancer cloning nanotag reporter vectorDepositorInsertFoxD3_cranial_neural_crest_enhancer_NC1
UseEnhancer cloning nanotag reporter vectorTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pChlr-7SE
Plasmid#52168PurposeEncodes module 7 with amino acids 12S-16E for recognition of nt 2GDepositorInsertPumilio homology domain amino acids 241-283 (PUM1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_EGFR
Plasmid#183285PurposeAll-in-One CRISPRko system with a guide RNA that targets EGFR geneDepositorInsertEGFR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin C431S
Plasmid#45976DepositorInsertParkin C431S (Prkn Rat)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromotertacAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBEAST2-sfGFP
Plasmid#126575PurposeStrong constitutive expression of super-folder GFP for cell-free expressionDepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_263
Plasmid#91127PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_SRC
Plasmid#183322PurposeAll-in-One CRISPRko system with a guide RNA that targets SRC geneDepositorInsertSRC
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_ABL2
Plasmid#183268PurposeAll-in-One CRISPRko system with a guide RNA that targets ABL2 geneDepositorInsertABL2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_ABL1
Plasmid#183267PurposeAll-in-One CRISPRko system with a guide RNA that targets ABL1 geneDepositorInsertABL1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFPmAID_htz-1
Plasmid#174214PurposeDonor plasmid for tagging htz-1 with GFP::mAIDDepositorInsertGFP::mAID::htz-1 tagging donor
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292-D570A
Plasmid#129679PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAaCas12b
UseCRISPR; Gateway compatible aacas12b entry cloneTagsExpressionPlantMutationD570A; AaCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP1054
Plasmid#119241Purposeconstruction intermediateDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJMP1050
Plasmid#119240Purposeconstruction intermediateDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_HDAC3
Plasmid#183299PurposeAll-in-One CRISPRko system with a guide RNA that targets HDAC3 geneDepositorInsertHDAC3
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFP.R271
Plasmid#138220PurposeExpresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_ESR1
Plasmid#183288PurposeAll-in-One CRISPRko system with a guide RNA that targets ESR1 geneDepositorInsertESR1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_DNMT1
Plasmid#183282PurposeAll-in-One CRISPRko system with a guide RNA that targets DNMT1 geneDepositorInsertDNMT1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_AAVS1B
Plasmid#183338PurposeAll-in-One CRISPRko system with a guide RNA that targets the AAVS1 locusDepositorInsertAAVS1B
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPPC020.306
Plasmid#171148PurposeExpression of J3-BBa_J23117-mRFP and J306 scRNA on pBBR1-GmR plasmidDepositorInsertsJ306 scRNA
J3-BBa_J23117-mRFP
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J3-BBa_J23117Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUPERretro-Sirt7 shRNA2
Plasmid#53145PurposeshRNAi vector for Sirt7DepositorInsertSirt7
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterH1 RNA polymerase IIIAvailable sinceMay 9, 2014AvailabilityAcademic Institutions and Nonprofits only