We narrowed to 12,148 results for: NSI
-
Plasmid#127411PurposeDouble mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethersDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
ExpressionBacterialMutationN346A-C348APromoteraraBADAvailable SinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMAH-POLY-eRF1(E55D)
Plasmid#157925PurposeEncodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cellsDepositorInsertPOLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
ExpressionMammalianPromoterCMVAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_sfGPF 150TAG
Plasmid#154765Purposeplasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAGPromoterEF1Available SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cyto-roGFP
Plasmid#49435PurposeExpresses thiol redox-sensitive ratiometric sensor roGFP in cytosol of mammalian cellsDepositorInsertroGFP2
UseAdenoviralExpressionMammalianMutationC48S, Q80R, S147C, and Q204C in Clontech's G…PromoterCMVAvailable SinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
DD-DamV133A-LMNB1-IRES2-mCherry
Plasmid#159599PurposeShield-1 inducible transient mammalian expression of Dam-LMNB1 for DamID, containing the V133A mutant of Dam.DepositorInsertDD-DamV133A-LMNB1-IRES2-mCherry
ExpressionMammalianMutationDam V133A mutationPromoterCMVAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_mScarlet_CLASP_RGS2membrane
Plasmid#133086PurposePlasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to blDepositorInsertmScarlet-CLASP
ExpressionBacterial and YeastAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUP-(2xCouRS-tRNAopt)
Plasmid#197930PurposeMachinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycineDepositorInsertsCouRS
tRNA opt
ExpressionBacterialPromoterglnS' and lambda(ts) and proKAvailable SinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0
Plasmid#174081PurposeExpression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.DepositorInsertNES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
TagsNES-FlagExpressionMammalianMutationL270G, Y271L, N311G, C313APromoterCMVAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pcDNA3.1-NINJ1
Plasmid#201180PurposeTransient expression of NINJ1 gene in mammalian cellsDepositorAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG NC
Plasmid#214675PurposeNegative control plasmid for splicing reporters consisting of MCP fused FLAG epitope tagsDepositorInsertFLAG
TagsMCP and V5ExpressionMammalianPromoterEF-1aAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
GAPDH-CPG-TV
Plasmid#70148Purposea targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyADepositorInsert5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
UseMouse Targeting and Synthetic BiologyAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSupAR-MbPylRS(DiZPK)
Plasmid#91705Purposeexpress Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interestDepositorInsertspylS
pylT
ExpressionBacterialMutationT810G, C813T, C820G, T821C, T937A, A1046T, G1257AAvailable SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHcRed-NPM1wt-C1
Plasmid#131818PurposeExpresses HcRed-NPM1wildtype (human) in mammalian cells through transient transfectionDepositorAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-NPM1wt
Plasmid#131810PurposeExpresses Flag-NPM1wildtype (human) in mammalian cells through transient transfectionDepositorAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMito-MtrxRFP2
Plasmid#172324Purposefluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)DepositorInsertmitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
TagsHis-tag and Mitochondria localization sequenceExpressionMammalianPromoterCMV, SP6Available SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK/CMV)-12xHRE
Plasmid#172335Purposehypoxic responsive reporterDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianPromoterPGK/CMVAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330-EN1201
Plasmid#92144PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse TIGRE acceptor locusDepositorInsertspCas9-nuclease and sgRNA against mouse TIGRE acceptor locus
ExpressionMammalianAvailable SinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-EGFP-FP4-mito
Plasmid#229692PurposeTransient expression of EGFP FP4-mito in mammalian cellsDepositorInsertEGFP; Mitochondira FP4 motifs from Listeria ActA
TagsEGFPExpressionMammalianPromoterCMV promoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJOG1368
Plasmid#167301PurposeLevel 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-FluorocytosineDepositorInsertFCY-UPP fusion gene
UseSynthetic Biology; Moclo level 0 vectorAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only