Addgene

We narrowed to 6,930 results for: Cad

Showing: 2551 - 2600 of 6930 results

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pAAV-hSyn-Chronos-mT-Sapphire

Plasmid

#149690

Purpose

For packaging and expression of Chronos-mT-Sapphire in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

Chronos

Use

AAV

Tags

mT-Sapphire

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pNES-VL12.3

Plasmid

#43862

Depositor

Dane Wittrup

Article

Colby et al Proc Natl Acad Sci U S A. 2004 Dec 21;10

Insert

VL12.3

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2013

Availability

Academic Institutions and Nonprofits only
plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209726

Purpose

Expresses cytosolic medium affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CamKII

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Clathrin-LCa-EYFP

Plasmid

#21741

Depositor

Xiaowei Zhuang

Article

Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pLVE-eGFP

Plasmid

#52581

Purpose

Lentiviral transfer vector driving expression of eGFP in mammalian cells

Depositor

Charles Gersbach

Article

Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

Insert

eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1alpha

Available since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

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psiTE(2)-Gal4

Plasmid

#220451

Purpose

Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(0)-Gal4DBD

Plasmid

#220452

Purpose

Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4DBD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(2)-Gal4DBD

Plasmid

#220454

Purpose

Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4DBD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiTE(1)-p65AD

Plasmid

#220456

Purpose

Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-p65AD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(0)-Gal4

Plasmid

#220449

Purpose

Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiTE(2)-LexAGAD

Plasmid

#220460

Purpose

Split intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-LexAGAD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiTE(1)-Gal4DBD

Plasmid

#220453

Purpose

Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4DBD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiTE(1)-LexAGAD

Plasmid

#220459

Purpose

Split intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-LexAGAD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiTE(0)-p65AD

Plasmid

#220455

Purpose

Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-p65AD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiTE(1)-Gal4

Plasmid

#220450

Purpose

Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiTE(0)-LexAGAD

Plasmid

#220458

Purpose

Split intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-LexAGAD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiTE(2)-p65AD

Plasmid

#220457

Purpose

Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-p65AD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p6618 MSCV-IP N-HAonly WHSC2

Plasmid

#35014

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

WHSC2 (NELFA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

Feb. 9, 2012

Availability

Academic Institutions and Nonprofits only
pHW522 (Prab-3::cGAL-C::let-858 3'UTR)

Plasmid

#107130

Purpose

cGAL-C driver under the control of C. elegans rab-3 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::gp41-1-C-intein::cGAL(AD)

Use

Tags

Expression

Worm

Mutation

Promoter

C. elegans rab-3 promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p6592 MSCV-IP N-HAonly WWP2

Plasmid

#34905

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

WWP2 (WWP2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only
psicheck-2-Acsm2

Plasmid

#48158

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of Acsm2 (Acsm2 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-FlagBcl10

Plasmid

#18718

Depositor

Jon Ashwell

Article

Wu et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

B cell lymphoma 10 (BCL10 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pET26b-Granzyme A (S-A)

Plasmid

#8825

Depositor

Judy Lieberman

Article

Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

Insert

Human Granzyme A inactive (GZMA Human)

Use

Tags

(His)6

Expression

Bacterial

Mutation

changed Serine 184 to Alanine

Promoter

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only
pCAGGS-BMAL1

Plasmid

#186824

Purpose

Expresses BMAL1 in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

bmal1 (Bmal1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKL002

Plasmid

#98921

Purpose

pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.

Depositor

Joel Kralj

Article

Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

Inserts

PROPS
GCaMP6f

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-JNKAR1

Plasmid

#61625

Purpose

FRET-based biosensor for monitoring JNK activity

Depositor

Jin Zhang

Article

Fosbrink et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

JNKAR1

Use

Tags

Citrine and ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Luc-SIRT1 3'UTR

Plasmid

#20379

Depositor

Charles Lowenstein

Article

Yamakuchi et al Proc Natl Acad Sci U S A. 2008 Sep 9. 10

Insert

SIRT1 (SIRT1 Human)

Use

Luciferase

Tags

Expression

Mutation

3'UTR of SIRT1

Promoter

Available since

May 1, 2009

Availability

Academic Institutions and Nonprofits only
pFasBac+GFP-CAMSAP1

Plasmid

#59036

Purpose

for baculovirus expression of CAMSAP1 in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP1 (CAMSAP1 Human)

Use

Tags

His and eGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
HBT_pcoCASphi_version1

Plasmid

#197931

Purpose

Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pcoCasphi-2

Use

CRISPR

Tags

FLAG tag and SV40 NLS and nucleoplasmin NLS

Expression

Plant

Mutation

Promoter

35SPPDK promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
35S-DsREDmonomer-NosT

Plasmid

#79183

Purpose

Transient expression vector for DsRED monomer

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Proc Natl Acad Sci U S A. 2010 Nov 9;107

Type

Empty backbone

Use

Reporter plasmid

Tags

DsREDmonomer

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pXL004-ishRNA-scramble

Plasmid

#36311

Depositor

Sean Palecek

Article

Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

Insert

shRNA of scramble

Use

Lentiviral and RNAi

Tags

EF1a-TetR-IRES-RFP-Puro

Expression

Mammalian

Mutation

Promoter

TetO-H1

Available since

July 9, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPCX-PECAMTS

Plasmid

#45850

Depositor

Martin Schwartz

Article

Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

Insert

PECAM1 (PECAM1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBPhsFlp2::PEST

Plasmid

#63171

Purpose

Drosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock Promoter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

hsFlp2::PEST

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFasBac+GFP-CAMSAP3

Plasmid

#59038

Purpose

for baculovirus expression of mouse CAMSAP3 in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP3 (Camsap3 Mouse)

Use

Tags

His and eGFP

Expression

Insect

Mutation

Promoter

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW-AlphaLobe

Plasmid

#113255

Purpose

E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

Depositor

Jennifer Doudna

Article

Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

S. pyogenes Cas9 alpha-helical lobe

Use

Tags

10xHis-MBP

Expression

Bacterial

Mutation

residues 56–714; See paper for description

Promoter

T7

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-CoilR-Cys

Plasmid

#181989

Purpose

Used to label proteins with the CoilE genetically encoded tag

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

CoilR-Cys

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pXWJ109Hg-gfp

Plasmid

#123148

Purpose

A mercury sensor with optimized intracellular receptor density. pSB3K3 carrying J109-32merR-t-PmerT-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-32merR-t-PmerT-30gfp-t

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J109, PmerT

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKL004

Plasmid

#98920

Purpose

Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.

Depositor

Joel Kralj

Article

Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

Insert

GCaMP6f tethered to mRuby

Use

Tags

His and mRuby3

Expression

Bacterial

Mutation

Promoter

BBa_J23118 - Strong Constitutive

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
As4

Plasmid

#123161

Purpose

Arsenic sensor used in the sensor cell array. pSB4A3 carrying J115-30arsR-t-ParsR-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J115-30arsR-t-ParsR-30gfp-t

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J115, ParsR

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB2_mScarletI_mVenusNB-MrkH

Plasmid

#182291

Purpose

expresses c-di-GMP biosensor in E. coli

Depositor

Howard Berg

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

Insert

mVenusNB-MrkH

Use

Tags

mVenusNB internal fusion to MrkH

Expression

Bacterial

Mutation

Promoter

proC

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS426 Gal TDP43

Plasmid

#27466

Depositor

Aaron Gitler

Article

Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

Insert

TDP-43 (TARDBP Human)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only
819 pGEX2T PTEN C124S

Plasmid

#10745

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

C124S

Promoter

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
Wild-type CoV-2 ORF8

Plasmid

#159933

Purpose

Protein overexpression in E.coli

Depositor

James Hurley

Article

Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

Insert

CoV-2 ORF8 18-121 (ORF8 CoV-2)

Use

Tags

6x Histidine, TEV cleavable

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGSTag Bmf

Plasmid

#24264

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (BMF Human)

Use

Tags

Gst

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
p416 103Q GAL

Plasmid

#1186

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 103Q (HTT Human)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
PXWABS62(E11-TEV)

Plasmid

#123157

Purpose

For TEV-based leakiness regulation. pSB4A3 carrying ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

ParsR-ABS62, Pecf11

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
882 pSG5L HA PTEN G129E

Plasmid

#10746

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Use

Tags

Expression

Mammalian

Mutation

G129E

Promoter

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
mEm-CoilE-Actin

Plasmid

#181993

Purpose

CoilE genetically encoded tag on mEmerald-Actin, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

mEm-CoilE-Actin

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE2-Bla(HA–RILP)

Plasmid

#102425

Purpose

HA tag fused to the N-terminus of RILP for the expression in mammalian cells.

Depositor

Steven Weinman

Article

Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

Rab interacting lysosomal protein (RILP Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Tet-responsive

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p6642 MSCV-P C-FlagHA 6bE7-Kozak

Plasmid

#35020

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV6b E7 (E7 HPV 6b)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

MSCV LTR

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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