We narrowed to 18,824 results for: nls

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pAAV-hDlx-GqDREADD-dTomato-Fishell-4

Plasmid

#83897

Purpose

Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

Depositor

Gordon Fishell

Publication

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has service

AAV1, AAV9, and AAV Retrograde

Insert

hM3Dq

Use

AAV

Expression

Mammalian

Promoter

hDlx

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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MSCV_Cas9_puro

Plasmid

#65655

Purpose

Retroviral introduction of Cas9 into mammalian cell line.

Depositor

Christopher Vakoc

Publication

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Insert

Cas9

Use

CRISPR and Retroviral

Tags

5 ' FLAG tag

Expression

Mammalian

Promoter

MSSV_LTR

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pnos-Cas9-nos

Plasmid

#62208

Purpose

Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.

Depositor

Simon Bullock

Publication

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

cas9

Expression

Insect

Promoter

nanos

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pcDNA3.3_mCherry

Plasmid

#26823

Depositor

Derrick Rossi

Publication

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

5'UTR-mCherry-3'UTR

Expression

Mammalian

Available since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pC035 - dLwCas13a-msfGFP

Plasmid

#91925

Purpose

Expresses catalytically inactive LwCas13a for mammalian RNA targeting

Depositor

Feng Zhang

Publication

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dLwCas13a

Use

CRISPR

Tags

NLS-3xHA and NLS-msfGFP

Expression

Mammalian

Mutation

R488A and R1060A

Promoter

EF-1a

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCS2-Cas9

Plasmid

#47322

Purpose

For in vivo expression of Cas9 or generation of Cas9 mRNA

Depositor

Alex Schier

Publication

Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Use

CRISPR; Xenopus and zebrafish expression

Expression

Mammalian

Promoter

SP6

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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M-NMn-VP64

Plasmid

#48676

Purpose

Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized

Depositor

George Church

Publication

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9-VP64, nuclease-null

Use

CRISPR

Tags

NLS and VP64

Mutation

nuclease-null

Promoter

CMV

Available since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLOX-CWBmi1

Plasmid

#12240

Depositor

Didier Trono

Publication

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

Bmi1 (Bmi1 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

CMV promoter upstream of mBmi-1. LoxP in 3' …

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pJM503 EF1α 3xFLAG-NLS-ZF1 in TUPV2

Plasmid

#161534

Purpose

Constitutive expression of 3xFLAG-NLS-ZF1 under the EF1α promoter

Depositor

Joshua Leonard

Publication

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-ZF1

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

EF1α

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-MCS-MED1long-IDR-YFP-NLS

Plasmid

#145276

Purpose

Mammalian Expression of Nuclear MED1 long IDR-YFP with SV40 NLS

Depositor

Denes Hnisz

Publication

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MED1-IDR long (MED1 Human)

Expression

Mammalian

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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v5 Cas9-eVLP

Plasmid

#228468

Purpose

Expresses MMLVgag(C507V)–Cas9 for producing v5 Cas9-eVLPs

Depositor

David Liu

Publication

Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MMLVgag(C507V)–Cas9

Tags

FLAG

Expression

Mammalian

Mutation

MMLVgag(C507V)

Promoter

CMV

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMK1334

Plasmid

#127965

Purpose

sgRNA expression vector compatible with CROP-Seq and imaging screens

Depositor

Martin Kampmann

Publication

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pTRIP-CMV-mCherry-53BP1

Plasmid

#127658

Purpose

Over-express mCherry-53BP1 fragment to monitor 53BP1 foci.

Depositor

Nicolas Manel

Publication

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

53BP1 (TP53BP1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

Nucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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xCas9 3.6

Plasmid

#108384

Purpose

Mammalian expression vector for xCas9 3.6

Depositor

David Liu

Publication

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

xCas9 3.6

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMSCVpuro-Cre

Plasmid

#34564

Purpose

Retroviral expression of Cre

Depositor

Kai Ge

Publication

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Cre

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

5'LTR

Available since

Jan. 25, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Snail_pGL2

Plasmid

#31694

Depositor

Paul Wade

Publication

Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Snail (SNAI1 Human)

Use

Luciferase

Expression

Mammalian

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pGWT35S-Citrine-NLS

Plasmid

#182583

Purpose

Transient expression of Citrine-NLS in plant cell (Nucleus)

Depositor

Yutaka Kodama

Publication

Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

Insert

NLS

Tags

Citrine

Expression

Plant

Promoter

CaMV 35S promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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P4-PE

Plasmid

#211375

Purpose

Packaging plasmid for v3b PE-eVLP production

Depositor

David Liu

Publication

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

P4-PE

Expression

Mammalian

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRT043-CLYBL-DDdcas9-VPH

Plasmid

#158091

Purpose

Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

Depositor

Martin Kampmann

Publication

Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CLYBL-CAG-DDdCas9VPH-T2A-GFP

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1-hLbCpf1(TYCV) (pY230)

Plasmid

#89355

Purpose

Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

Depositor

Feng Zhang

Publication

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

G532R/K595R

Promoter

CMV

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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