We narrowed to 6,231 results for: nls

1. pAAV-S5E8-dTom-nlsdTom

   Plasmid

   #135643

   Purpose

   AAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E8 regulatory element

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Insert

   dTom-nlsdTom
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   Available Since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

   Plasmid

   #85821

   Purpose

   iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   Zf Ubi::cMyc oDam_f_GFPoNLS
   

   Use

   Tol2 based vector

   Tags

   cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…

   Expression

   Mutation

   L122A. Codon optimization compatible to work in M…

   Promoter

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. GST-FUS ΔNLS 1-513

   Plasmid

   #44984

   Depositor

   Robin Reed

   Article

   Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

   Insert

   FUS ΔNLS (1-513aa) (FUS Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains amino acids 1-513

   Promoter

   tac

   Available Since

   June 3, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pT2-7xTcf-NLS-YNL

   Plasmid

   #65711

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, YNL
   

   Use

   Luciferase; Tol2 system

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGGEV_2_+nls-eGFP-HA-_+1_BK+

   Plasmid

   #49312

   Purpose

   Golden Gate entry vector: Position 2, HA tagged nls-EGFP with start and stop codon

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Insert

   nls-EGFP-HA
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC

   Plasmid

   #67687

   Purpose

   UAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassette

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3796-38

   Insert

   dusp6 (dusp6 Zebrafish)
   

   Use

   Tags

   6xmyc-NLS-tdTomato-2A

   Expression

   Mutation

   S159A, S196A

   Promoter

   Available Since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. PHO4NLS-GFP-PRA (109)

   Plasmid

   #24044

   Depositor

   Michael Rout

   Insert

   PHO4
   

   Use

   Tags

   EGFP and PrA

   Expression

   Yeast

   Mutation

   Only bp 421-588 of PHO4 (NLS)

   Promoter

   Available Since

   May 3, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pPHR-EYFP-NLS-VP16

   Plasmid

   #103822

   Purpose

   Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-EYFP-VP16 (CRY2 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   VP16: G126T (silent, G42G)

   Promoter

   CMV

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. NAB2NLS-GFP-PRA (107)

   Plasmid

   #24043

   Depositor

   Michael Rout

   Insert

   NAB2
   

   Use

   Tags

   EGFP and PrA

   Expression

   Yeast

   Mutation

   Only bp 601-750 of NAB2 (NLS)

   Promoter

   Available Since

   May 3, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pEBB-GFP-hBEN-deltaNLS3

    Plasmid

    #22158

    Depositor

    Ananda Roy

    Article

    Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

    Insert

    hBEN (GTF2IRD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 883-889

    Promoter

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pME-DRE-nls (JDW 31)

    Plasmid

    #242561

    Purpose

    Gateway middle entry clone containing Dre recombinase

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    codon optimized DRE-nls
    

    Use

    Gateway subcloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSS22-IRES-NLS-eGFP

    Plasmid

    #241837

    Purpose

    Part I of the Donor construct for UBE3A reporter

    Depositor

    Albert Keung

    Article

    Kelkar et al Front Cell Dev Biol. 2025 Aug 29;13:1665

    Insert

    eGFP
    

    Use

    Donor plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-GRP78-NLS-Mut

    Plasmid

    #235206

    Purpose

    Expresses GRP78 with mutated NLS in mammalian cells

    Depositor

    Amy Shiu Lee

    Article

    Liu et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Mutate the NLS sequence

    Promoter

    Available Since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTrcHisA-NLS-BFP-mEosEM

    Plasmid

    #218934

    Purpose

    Express the nuclear import substrate NLS-BFP-mEosEM.

    Depositor

    Siegfried Musser

    Article

    Sau et al Nature. 2025 Mar 19. doi: 10.1038/s41586

    Insert

    BFP-mEosEM fusion protein
    

    Use

    Tags

    6xHis and SV40 nuclear localization sequence (NLS…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. UBQ10pro-nlsABACUS2-400n-NosT

    Plasmid

    #203728

    Purpose

    Binary vector for the transformation of the nlsABACUS2-400n biosensor for (+)-Abscisic acid into Arabidopsis

    Depositor

    Alexander Jones

    Article

    Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

    Insert

    nlsABACUS2-400n
    

    Use

    Synthetic Biology

    Tags

    Myc

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10pro 1500bp

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCI5-CasRx-NLS-HA (pKW919)

    Plasmid

    #229047

    Purpose

    Mammalian expression of CasRx-NLS-HA

    Depositor

    S Chul Kwon

    Article

    Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

    Insert

    CasRx (RfxCas13d)
    

    Use

    CRISPR

    Tags

    NLS and NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV_Zdk3-VPR_P2A_dCas9-AsLOVx3-NLSx3

    Plasmid

    #231568

    Purpose

    Expresses components of the LOOMINA optogenetic transcriptional control system.

    Depositor

    Dominik Niopek

    Article

    Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

    Insert

    All-in-one LOOMINA construct
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-APEX2-SOPP3-3xNLS

    Plasmid

    #229074

    Purpose

    expresses APEX2-SOPP3 in the mammalian nucleus

    Depositor

    Weijun Pan

    Article

    Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

    Insert

    Express APEX2-SOPP3 in the mammalian nucleus (APEX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. CPH3375_SF_Spt6p-tSH2-1223-1451_NLS

    Plasmid

    #227084

    Purpose

    Gal1p dependent budding yeast expression of tandem-affinity tagged spt6p tSH2 domain (1123-1451) localized to nucleus

    Depositor

    Chris Hill

    Insert

    spt6-tsh2 (SPT6 Budding Yeast)
    

    Use

    Tags

    STREPii-FLAG and SV40 NLS 1

    Expression

    Yeast

    Mutation

    Promoter

    Gal1-10

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-AGG-NLS-EGFP

    Plasmid

    #214602

    Purpose

    express EGFP ; in vitro transcribed modified RNA vector

    Depositor

    Yuxuan Guo

    Article

    Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

    Insert

    EGFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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