We narrowed to 6,508 results for: nls

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pMH-Flag-HA

Plasmid

#101766

Purpose

Gateway-compatible destination vector for mammalian expression of N-terminal HA & Flag-tag fusion protein

Depositor

Michael Huen

Article

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Type

Empty backbone

Tags

Flag-HA

Expression

Mammalian

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pSBbi-FoxO1_1R_13A_3D

Plasmid

#106279

Purpose

Fluorescent fusion protein for FoxO1

Depositor

Laura Heiser

Article

Gross et al Cell Syst. 2019 Dec 18;9(6):580-588.e4.

Inserts

Foxo1a (Foxo1 Mouse)
NLS-mCherry-NLS-P2A-Puromycin

Tags

Clover fluorescent protein and NLS

Expression

Mammalian

Mutation

Deleted amino acids 401-636, T24A, S209A, H212R, …

Promoter

EF1a/RPBSA

Available Since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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PB_SBE4:mScarlet-I-NLS

Plasmid

#154961

Purpose

encodes transcriptional reporter of SMAD2/3

Depositor

Ewa Bielczyk-Maczynska, Joshua Knowles

Article

Taylor et al Sci Rep. 2020 Aug 21;10(1):14084. doi: 1

Insert

mScarlet

Use

Piggybac

Tags

NLS-PEST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-1BPNLS-Cas9-1BPNLS-2AGFP

Plasmid

#87109

Purpose

BPNLS-Cas9-BPNLS-2AGFP expression plasmid

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

1BPNLS-Cas9-1BPNLS-2A-EGFP

Use

CRISPR

Tags

3xFLAG (on Cas9)

Expression

Mammalian

Promoter

CAG

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xFLAG-NLS-TPP1

Plasmid

#53585

Purpose

Mammalian expression of 3xFLAG tagged, nuclear localized TPP1, N-terminal.

Depositor

Steven Artandi

Article

Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

Insert

TPP1 (ACD Human)

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Silent mutation to eliminate PacI site

Promoter

CMV

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-dLbCpf1-BE-YE1

Plasmid

#154145

Purpose

Expresses dLbCpf1-BE-YE1(dLbCas12a-BE-YE1) in mammalian cells.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

Insert

dLbCpf1-BE-YE1 (dCas12a-BE-YE1)

Tags

SV40 NLS

Expression

Mammalian

Mutation

APOBEC1(W90Y+R126E), dLbCpf1(D832A+E925A+D1148A)

Promoter

CMV

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Prab-3::NLS::GFP

Plasmid

#68120

Purpose

Nuclear Localized GFP for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

GFP

Expression

Worm

Promoter

rab-3

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pREP3-TEV-NLS

Plasmid

#21146

Depositor

Stephen Kearsey

Article

Yang et al BMC Mol Biol. 2005 Jun 7;6:13. doi: 10.1

Insert

TEV protease (TEVgp1 tobacco etch virus)

Use

S. pombe

Tags

2xNLS, NLS, and myc

Expression

Yeast

Available Since

July 6, 2009

Availability

Academic Institutions and Nonprofits only

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ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-MCS:

Plasmid

#179585

Purpose

ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette (Red module-dsRed/Green module-NeonGreen)

Depositor

Ali Hemmati Brivanlou

Article

De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

Insert

NLS-NG-T2A-hSHH

Use

Piggybac

Tags

NLS-NG-T2A

Promoter

CAGp

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Akt-STOPS-NLS

Plasmid

#175007

Purpose

Nuclear-targeted Akt-STOPS.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

mCherry-Akt-STOPS-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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3XFLAG-VP64-SadCas9-NLS-VP64

Plasmid

#135338

Purpose

3xFLAG 2xVP64 SadCas9 for single guide cloning

Depositor

Ronald Cohn

Article

Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

Insert

SadCas9

Tags

3xFLAG-VP64-SV40 NLS and NLS-VP64

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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CMV Marina-T2A-nls-mCherry

Plasmid

#74216

Purpose

genetically-encoded fluorescent voltage indicator

Depositor

Vincent Pieribone

Article

Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

Insert

fluorescent protein voltage sensor

Tags

mCherry

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pKM065

Plasmid

#134175

Purpose

Retroviral plasmid expressing GFP-NLS-Plk1-PBD

Depositor

Iain Cheeseman

Article

McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

Insert

GFP-NLS-Plk1-PBD 326-603 (PLK1 Human)

Use

Retroviral

Tags

eGFP-TEV-S

Expression

Mammalian

Mutation

PBD 326-603

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pRD400

Plasmid

#163107

Purpose

Expression of eYFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

NLS_eYFP_H-NSdbd_NLS

Use

Eukaryotic expression

Expression

Mammalian

Promoter

CMV promoter; T7 promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Fuw-AcrIIA4-P2A-GFP

Plasmid

#108247

Purpose

Lentiviral vector to express AcrIIA4-P2A-GFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

AcrIIA4-NLS-HA (NEWENTRY Synthetic)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

Ubc

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCambia2300-Aar1-ccdB-NLS2xmCH

Plasmid

#254406

Purpose

Destination vector for bifluorescence complementation. The plasmid carries NLS-mCherry expressed from the Medicago BCP1promoter.

Depositor

Maria Harrison

Article

Ivanov et al New Phytol. 2026 Feb;249(4):1592-1604. d

Insert

NLS 2X mCherry expressed from the BCP1 promoter

Expression

Plant

Promoter

Mt BCP1

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pDonor-tBFP-NLS-Neo

Plasmid

#80766

Purpose

Customizable donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Type

Empty backbone

Use

CRISPR

Tags

TagBFP2-3×NLS

Expression

Mammalian

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-tagRFP-NLS-HA-Bxb1

Plasmid

#65625

Purpose

Vector for cotranslational expression of tagRFP-Bxb1

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Bxb1 integrase

Tags

HA, SV40 NLS, and tagRFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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ePPE-V223A

Plasmid

#205243

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

SV40_NLS-nCas9-NC-MLV-SV40_NLS

Use

CRISPR

Expression

Plant

Mutation

Detailed in manuscript

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDSP33

Plasmid

#199375

Purpose

Encodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expression

Depositor

Hang Lu

Insert

NLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS

Expression

Bacterial

Mutation

no mutations, except added subcellular localizati…

Available Since

April 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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